KEGG   ORTHOLOGY: K18896
Entry
K18896                      KO                                     

Name
gsmt
Definition
glycine/sarcosine N-methyltransferase [EC:2.1.1.156]
Pathway
ko00260  Glycine, serine and threonine metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00260 Glycine, serine and threonine metabolism
    K18896  gsmt; glycine/sarcosine N-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.156  glycine/sarcosine N-methyltransferase
     K18896  gsmt; glycine/sarcosine N-methyltransferase
Other DBs
RN: R10060
COG: COG0500
GO: 0017174 0052730
Genes
PFS: PFLU_2095
PTV: AA957_04385
PAZO: AYR47_17170
PFW: PF1751_v1c19160
PFF: PFLUOLIPICF727140
PFX: A7318_08950
TIG: THII_1900 THII_1901
TMB: Thimo_1798 Thimo_2027
MPUR: MARPU_03465
TEE: Tel_06780
TGR: Tgr7_1181
TKM: TK90_0179
TVR: TVD_00875
CSA: Csal_0742
SALN: SALB1_0939
BCON: NL30_36005
MXA: MXAN_7068
HOH: Hoch_6748
PLA: Plav_2856
RBS: RHODOSMS8_01653(bsmA)
PSF: PSE_p0300
GAK: X907_0699
AAY: WYH_00219
MAGQ: MGMAQ_3234
BMYC: DJ92_2202
BSE: Bsel_2039
BTS: Btus_2799
AMT: Amet_1179
AOE: Clos_0075
CTH: Cthe_1959
CCE: Ccel_1149
DRM: Dred_0699
CTHM: CFE_1976
FMA: FMG_0285
SHY: SHJG_1835
SALU: DC74_857
SALL: SAZ_04785
STRE: GZL_00112
SLX: SLAV_39415(bsmA)
SEN: SACE_5862(gsmT)
PAUT: Pdca_14800
SYW: SYNW1914(gsmt)
SYNR: KR49_11730
SYNW: SynWH8103_02187(gbmt1)
PMT: PMT_0552
ATM: ANT_00580
CTS: Ctha_2188
MRO: MROS_1219
MMH: Mmah_0524
MPD: MCP_2436
MEZ: Mtc_2203
RCI: RCIX2438
AG: AAF87202(gsmt) BAC56939(gsmt)
 » show all
Reference
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence

KEGG   ORTHOLOGY: K24071
Entry
K24071                      KO                                     

Name
gsmt-sdmt
Definition
glycine/sarcosine/dimethylglycine N-methyltransferase [EC:2.1.1.156 2.1.1.157]
Pathway
ko00260  Glycine, serine and threonine metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00260 Glycine, serine and threonine metabolism
    K24071  gsmt-sdmt; glycine/sarcosine/dimethylglycine N-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.156  glycine/sarcosine N-methyltransferase
     K24071  gsmt-sdmt; glycine/sarcosine/dimethylglycine N-methyltransferase
    2.1.1.157  sarcosine/dimethylglycine N-methyltransferase
     K24071  gsmt-sdmt; glycine/sarcosine/dimethylglycine N-methyltransferase
Other DBs
RN: R10060 R10061
COG: COG0500
GO: 0017174 0052730 0052729
Genes
SALH: HMF8227_02086(gsmT)
NIY: FQ775_18170
RBM: TEF_00780
LAP: ACP90_08120
LAGG: B0E33_20175
LABR: CHH27_01290
LABT: FIU93_13755
RID: RIdsm_00793
MALU: KU6B_49030
MMI: MMAR_0861
SMAL: SMALA_5460
SEN: SACE_3901
SNA: Snas_2966
 » show all
Reference
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence

KEGG   ENZYME: 2.1.1.156
Entry
EC 2.1.1.156                Enzyme                                 

Name
glycine/sarcosine N-methyltransferase;
ApGSMT;
glycine-sarcosine methyltransferase;
GSMT;
GMT;
glycine sarcosine N-methyltransferase;
S-adenosyl-L-methionine:sarcosine N-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:glycine(or sarcosine) N-methyltransferase [sarcosine(or N,N-dimethylglycine)-forming]
Reaction(IUBMB)
2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine (overall reaction) [RN:R10060];
(1a) S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine [RN:R00367];
(1b) S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine [RN:R07243]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
glycine [CPD:C00037];
sarcosine [CPD:C00213]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N,N-dimethylglycine [CPD:C01026];
sarcosine [CPD:C00213]
Comment
Cells of the oxygen-evolving halotolerant cyanobacterium Aphanocthece halophytica synthesize betaine from glycine by a three-step methylation process. This is the first enzyme and it leads to the formation of either sarcosine or N,N-dimethylglycine, which is further methylated to yield betaine (N,N,N-trimethylglycine) by the action of EC 2.1.1.157, sarcosine/dimethylglycine N-methyltransferase. Differs from EC 2.1.1.20, glycine N-methyltransferase, as it can further methylate the product of the first reaction. Acetate, dimethylglycine and S-adenosyl-L-homocysteine can inhibit the reaction [3].
History
EC 2.1.1.156 created 2005
Pathway
ec00260  Glycine, serine and threonine metabolism
ec01100  Metabolic pathways
Orthology
K18896  glycine/sarcosine N-methyltransferase
K24071  glycine/sarcosine/dimethylglycine N-methyltransferase
Genes
PFS: PFLU_2095
PTV: AA957_04385
PAZO: AYR47_17170
PFW: PF1751_v1c19160
PFF: PFLUOLIPICF727140
PFX: A7318_08950
SALH: HMF8227_02086(gsmT)
TIG: THII_1900 THII_1901
TMB: Thimo_1798 Thimo_2027
MPUR: MARPU_03465
TEE: Tel_06780
TGR: Tgr7_1181
TKM: TK90_0179
TVR: TVD_00875
CSA: Csal_0742
SALN: SALB1_0939
BCON: NL30_36005
MXA: MXAN_7068
HOH: Hoch_6748
PLA: Plav_2856
RBS: RHODOSMS8_01653(bsmA)
RBM: TEF_00780
PSF: PSE_p0300
MALU: KU6B_49030
GAK: X907_0699
AAY: WYH_00219
MAGQ: MGMAQ_3234
BMYC: DJ92_2202
BSE: Bsel_2039
BTS: Btus_2799
AMT: Amet_1179
AOE: Clos_0075
CTH: Cthe_1959
CCE: Ccel_1149
DRM: Dred_0699
CTHM: CFE_1976
FMA: FMG_0285
MMI: MMAR_0861
SHY: SHJG_1835
SALU: DC74_857
SALL: SAZ_04785
STRE: GZL_00112
SMAL: SMALA_5460
SLX: SLAV_39415(bsmA)
SEN: SACE_3901 SACE_5862(gsmT)
PAUT: Pdca_14800
SNA: Snas_2966
SYW: SYNW1914(gsmt)
SYNR: KR49_11730
SYNW: SynWH8103_02187(gbmt1)
PMT: PMT_0552
ATM: ANT_00580
CTS: Ctha_2188
MRO: MROS_1219
MMH: Mmah_0524
MPD: MCP_2436
MEZ: Mtc_2203
RCI: RCIX2438
 » show all
Reference
1  [PMID:10896953]
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T.
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence
Reference
2  [PMID:11319079]
  Authors
Nyyssola A, Reinikainen T, Leisola M.
  Title
Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.
  Journal
Appl Environ Microbiol 67:2044-50 (2001)
DOI:10.1128/AEM.67.5.2044-2050.2001
  Sequence
Reference
3  [PMID:12466265]
  Authors
Waditee R, Tanaka Y, Aoki K, Hibino T, Jikuya H, Takano J, Takabe T, Takabe T.
  Title
Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.
  Journal
J Biol Chem 278:4932-42 (2003)
DOI:10.1074/jbc.M210970200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.156
IUBMB Enzyme Nomenclature: 2.1.1.156
ExPASy - ENZYME nomenclature database: 2.1.1.156
BRENDA, the Enzyme Database: 2.1.1.156
CAS: 294210-82-5

KEGG   REACTION: R10060
Entry
R10060                      Reaction                               

Name
S-adenosyl-L-methionine:glycine N-methyltransferase [N,N-dimethylglycine-forming]
Definition
2 S-Adenosyl-L-methionine + Glycine <=> 2 S-Adenosyl-L-homocysteine + N,N-Dimethylglycine
Equation
2 C00019 + C00037 <=> 2 C00021 + C01026
Reaction class
RC00003  C00019_C00021
RC03038  C00037_C01026
Enzyme
Pathway
rn00260  Glycine, serine and threonine metabolism
rn01100  Metabolic pathways
Orthology
K18896  glycine/sarcosine N-methyltransferase [EC:2.1.1.156]
K24071  glycine/sarcosine/dimethylglycine N-methyltransferase [EC:2.1.1.156 2.1.1.157]
Other DBs
RHEA: 32466

DBGET integrated database retrieval system