KEGG   ORTHOLOGY: K18897
Entry
K18897                      KO                                     

Name
sdmt
Definition
sarcosine/dimethylglycine N-methyltransferase [EC:2.1.1.157]
Pathway
ko00260  Glycine, serine and threonine metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00260 Glycine, serine and threonine metabolism
    K18897  sdmt; sarcosine/dimethylglycine N-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.157  sarcosine/dimethylglycine N-methyltransferase
     K18897  sdmt; sarcosine/dimethylglycine N-methyltransferase
Other DBs
RN: R10061
GO: 0052730 0052729
Genes
LCD: clem_04510
TIG: THII_1899
TMB: Thimo_1799
MPUR: MARPU_03460
TEE: Tel_06785
HHA: Hhal_1677
HHK: HH1059_14690
HHC: M911_05555
EBS: ECTOBSL9_2475
TGR: Tgr7_1180
TKM: TK90_0180
TVR: TVD_00880
SVA: SVA_2877
SALN: SALB1_0940
GME: Gmet_2958
GEO: Geob_0241
HOH: Hoch_6747
PLA: Plav_2857
RBM: TEF_13940
RHC: RGUI_2989
MALU: KU6B_49020
GAK: X907_0698
AAY: WYH_00220
SHUM: STHU_55140
MAGQ: MGMAQ_3233
MSHG: MSG_01046
STRE: GZL_06301
SLD: T261_1518
SALJ: SMD11_6312
SFK: KY5_7985c
SRO: Sros_5201
PAUT: Pdca_14790
ACTI: UA75_05195
ACAD: UA74_05190
MIL: ML5_5694
PROC: Ptc2401_00945(rebM)
CTS: Ctha_2187
MMH: Mmah_0525
MPL: Mpal_2451
HTU: Htur_0668
AG: AAF87203(sdmt) BAC56940(sdmt)
 » show all
Reference
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence

KEGG   ORTHOLOGY: K24071
Entry
K24071                      KO                                     

Name
gsmt-sdmt
Definition
glycine/sarcosine/dimethylglycine N-methyltransferase [EC:2.1.1.156 2.1.1.157]
Pathway
ko00260  Glycine, serine and threonine metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00260 Glycine, serine and threonine metabolism
    K24071  gsmt-sdmt; glycine/sarcosine/dimethylglycine N-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.156  glycine/sarcosine N-methyltransferase
     K24071  gsmt-sdmt; glycine/sarcosine/dimethylglycine N-methyltransferase
    2.1.1.157  sarcosine/dimethylglycine N-methyltransferase
     K24071  gsmt-sdmt; glycine/sarcosine/dimethylglycine N-methyltransferase
Other DBs
RN: R10060 R10061
COG: COG0500
GO: 0017174 0052730 0052729
Genes
SALH: HMF8227_02086(gsmT)
NIY: FQ775_18170
RBM: TEF_00780
LAP: ACP90_08120
LAGG: B0E33_20175
LABR: CHH27_01290
LABT: FIU93_13755
RID: RIdsm_00793
MALU: KU6B_49030
MMI: MMAR_0861
SMAL: SMALA_5460
SEN: SACE_3901
SNA: Snas_2966
 » show all
Reference
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence

KEGG   ENZYME: 2.1.1.157
Entry
EC 2.1.1.157                Enzyme                                 

Name
sarcosine/dimethylglycine N-methyltransferase;
ApDMT;
sarcosine-dimethylglycine methyltransferase;
SDMT;
sarcosine dimethylglycine N-methyltransferase;
S-adenosyl-L-methionine:N,N-dimethylglycine N-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:sarcosine(or N,N-dimethylglycine) N-methyltransferase [N,N-dimethylglycine(or betaine)-forming]
Reaction(IUBMB)
2 S-adenosyl-L-methionine + sarcosine = 2 S-adenosyl-L-homocysteine + betaine (overall reaction) [RN:R10061];
(1a) S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine [RN:R07243];
(1b) S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine [RN:R07244]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
sarcosine [CPD:C00213];
N,N-dimethylglycine [CPD:C01026]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
betaine [CPD:C00719];
N,N-dimethylglycine [CPD:C01026]
Comment
Cells of the oxygen-evolving halotolerant cyanobacterium Aphanocthece halophytica synthesize betaine from glycine by a three-step methylation process. The first enzyme, EC 2.1.1.156, glycine/sarcosine N-methyltransferase, leads to the formation of either sarcosine or N,N-dimethylglycine, which is further methylated to yield betaine (N,N,N-trimethylglycine) by the action of this enzyme. Both of these enzymes can catalyse the formation of N,N-dimethylglycine from sarcosine [3]. The reactions are strongly inhibited by S-adenosyl-L-homocysteine.
History
EC 2.1.1.157 created 2005, modified 2010
Pathway
ec00260  Glycine, serine and threonine metabolism
ec01100  Metabolic pathways
Orthology
K18897  sarcosine/dimethylglycine N-methyltransferase
K24071  glycine/sarcosine/dimethylglycine N-methyltransferase
Genes
SALH: HMF8227_02086(gsmT)
LCD: clem_04510
TIG: THII_1899
TMB: Thimo_1799
MPUR: MARPU_03460
TEE: Tel_06785
HHA: Hhal_1677
HHK: HH1059_14690
HHC: M911_05555
EBS: ECTOBSL9_2475
TGR: Tgr7_1180
TKM: TK90_0180
TVR: TVD_00880
SVA: SVA_2877
SALN: SALB1_0940
GME: Gmet_2958
GEO: Geob_0241
HOH: Hoch_6747
PLA: Plav_2857
RHC: RGUI_2989
GAK: X907_0698
AAY: WYH_00220
SHUM: STHU_55140
MAGQ: MGMAQ_3233
MMI: MMAR_0861
MSHG: MSG_01046
STRE: GZL_06301
SLD: T261_1518
SMAL: SMALA_5460
SALJ: SMD11_6312
SFK: KY5_7985c
SRO: Sros_5201
SEN: SACE_3901
PAUT: Pdca_14790
ACTI: UA75_05195
ACAD: UA74_05190
MIL: ML5_5694
SNA: Snas_2966
PROC: Ptc2401_00945(rebM)
CTS: Ctha_2187
MMH: Mmah_0525
MPL: Mpal_2451
HTU: Htur_0668
 » show all
Reference
1  [PMID:10896953]
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T.
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence
Reference
2  [PMID:11319079]
  Authors
Nyyssola A, Reinikainen T, Leisola M.
  Title
Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.
  Journal
Appl Environ Microbiol 67:2044-50 (2001)
DOI:10.1128/AEM.67.5.2044-2050.2001
  Sequence
Reference
3  [PMID:12466265]
  Authors
Waditee R, Tanaka Y, Aoki K, Hibino T, Jikuya H, Takano J, Takabe T, Takabe T.
  Title
Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.
  Journal
J Biol Chem 278:4932-42 (2003)
DOI:10.1074/jbc.M210970200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.157
IUBMB Enzyme Nomenclature: 2.1.1.157
ExPASy - ENZYME nomenclature database: 2.1.1.157
BRENDA, the Enzyme Database: 2.1.1.157

KEGG   REACTION: R10061
Entry
R10061                      Reaction                               

Name
S-adenosyl-L-methionine:sarcosine N-methyltransferase [betaine-forming]
Definition
2 S-Adenosyl-L-methionine + Sarcosine <=> 2 S-Adenosyl-L-homocysteine + Betaine
Equation
2 C00019 + C00213 <=> 2 C00021 + C00719
Reaction class
RC00003  C00019_C00021
RC03040  C00213_C00719
Enzyme
Pathway
rn00260  Glycine, serine and threonine metabolism
rn01100  Metabolic pathways
Orthology
K18897  sarcosine/dimethylglycine N-methyltransferase [EC:2.1.1.157]
K24071  glycine/sarcosine/dimethylglycine N-methyltransferase [EC:2.1.1.156 2.1.1.157]
Other DBs
RHEA: 32470

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