KEGG   ORTHOLOGY: K19104Help
Entry
K19104                      KO                                     

Name
nat
Definition
isonocardicin synthase [EC:2.5.1.38]
Pathway
ko00261  Monobactam biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00736  Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00261 Monobactam biosynthesis
    K19104  nat; isonocardicin synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.38  isonocardicin synthase
     K19104  nat; isonocardicin synthase
BRITE hierarchy
Other DBs
RN: R03072 R10881
GO: 0050007
Genes
AMI: Amir_4587
TaxonomyKoalaUniProt
Reference
PMID:9804844
  Authors
Reeve AM, Breazeale SD, Townsend CA
  Title
Purification, characterization, and cloning of an S-adenosylmethionine-dependent 3-amino-3-carboxypropyltransferase in nocardicin biosynthesis.
  Journal
J Biol Chem 273:30695-703 (1998)
DOI:10.1074/jbc.273.46.30695
Reference
  Authors
Gunsior M, Breazeale SD, Lind AJ, Ravel J, Janc JW, Townsend CA
  Title
The biosynthetic gene cluster for a monocyclic beta-lactam antibiotic, nocardicin A.
  Journal
Chem Biol 11:927-38 (2004)
DOI:10.1016/j.chembiol.2004.04.012

KEGG   ENZYME: 2.5.1.38Help
Entry
EC 2.5.1.38                 Enzyme                                 

Name
isonocardicin synthase;
nocardicin aminocarboxypropyltransferase;
S-adenosyl-L-methionine:nocardicin-E 3-amino-3-carboxypropyltransferase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:nocardicin-G 3-amino-3-carboxypropyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + nocardicin G = S-methyl-5'-thioadenosine + isonocardicin C [RN:R10881]
Reaction(KEGG)
R10881;
(other) R03072
Show
Substrate
S-adenosyl-L-methionine [CPD:C00019];
nocardicin G [CPD:C17355]
Product
S-methyl-5'-thioadenosine [CPD:C00170];
isonocardicin C [CPD:C17352]
Comment
The enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the beta-lactam antibiotic nocardicin A. The enzyme can act on nocardicin E, F, and G, producing isonocardicin A, B, and C, respectively. However, the in vivo substrate is believed to be nocardicin G [3].
History
EC 2.5.1.38 created 1992, modified 2016
Pathway
ec00261  Monobactam biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K19104  isonocardicin synthase
Genes
AMI: Amir_4587
Taxonomy
Reference
1
  Authors
Wilson, B.A., Bantia, S., Salituro, G.M., Reeve, A.M. and Townsend, C.A.
  Title
Cell-free biosynthesis of nocardicin A from nocardicin E and S-adenosylmethionine.
  Journal
J Am Chem Soc 110:8238-8239 (1988)
Reference
2  [PMID:9804844]
  Authors
Reeve AM, Breazeale SD, Townsend CA.
  Title
Purification, characterization, and cloning of an S-adenosylmethionine-dependent  3-amino-3-carboxypropyltransferase in nocardicin biosynthesis.
  Journal
J Biol Chem 273:30695-703 (1998)
DOI:10.1074/jbc.273.46.30695
Reference
3  [PMID:15629944]
  Authors
Kelly WL, Townsend CA.
  Title
Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.
  Journal
J Bacteriol 187:739-46 (2005)
DOI:10.1128/JB.187.2.739-746.2005
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.38
IUBMB Enzyme Nomenclature: 2.5.1.38
ExPASy - ENZYME nomenclature database: 2.5.1.38
BRENDA, the Enzyme Database: 2.5.1.38
CAS: 118246-74-5

KEGG   REACTION: R03072Help
Entry
R03072                      Reaction                               

Name
S-Adenosyl-L-methionine:nocardicin-E 3-amino-3-carboxypropyltransferase
Definition
S-Adenosyl-L-methionine + Nocardicin E <=> 5'-Methylthioadenosine + Isonocardicin A
Equation
Reaction class
RC00021  C00019_C00170
RC01372  C00927_C01739
Enzyme
Pathway
rn00261  Monobactam biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00736  Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A
Orthology
K19104  isonocardicin synthase [EC:2.5.1.38]
Other DBs
RHEA: 19848

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