KEGG   ORTHOLOGY: K19105Help
Entry
K19105                      KO                                     

Name
nocJ
Definition
nocardicin-A epimerase [EC:5.1.1.14]
Pathway
ko00261  Monobactam biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00736  Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00261 Monobactam biosynthesis
    K19105  nocJ; nocardicin-A epimerase
Enzymes [BR:ko01000]
 5. Isomerases
  5.1  Racemases and epimerases
   5.1.1  Acting on amino acids and derivatives
    5.1.1.14  nocardicin A epimerase
     K19105  nocJ; nocardicin-A epimerase
BRITE hierarchy
Other DBs
RN: R03073 R10883
GO: 0050143
Genes
AMI: Amir_4594
TaxonomyKoalaUniProt
Reference
  Authors
Kelly WL, Townsend CA
  Title
Mutational analysis and characterization of nocardicin C-9' epimerase.
  Journal
J Biol Chem 279:38220-7 (2004)
DOI:10.1074/jbc.M405450200
Reference
  Authors
Gunsior M, Breazeale SD, Lind AJ, Ravel J, Janc JW, Townsend CA
  Title
The biosynthetic gene cluster for a monocyclic beta-lactam antibiotic, nocardicin A.
  Journal
Chem Biol 11:927-38 (2004)
DOI:10.1016/j.chembiol.2004.04.012

KEGG   ENZYME: 5.1.1.14Help
Entry
EC 5.1.1.14                 Enzyme                                 

Name
nocardicin A epimerase;
isonocardicin A epimerase;
nocJ (gene name)
Class
Isomerases;
Racemases and epimerases;
Acting on amino acids and derivatives
BRITE hierarchy
Sysname
nocardicin-C epimerase
Reaction(IUBMB)
(1) isonocardicin C = nocardicin C [RN:R10883];
(2) isonocardicin A = nocardicin A [RN:R03073]
Reaction(KEGG)
Substrate
isonocardicin C [CPD:C17352];
isonocardicin A [CPD:C00927]
Product
nocardicin C [CPD:C17351];
nocardicin A [CPD:C01941]
Comment
Requires pyridoxal 5'-phosphate. The enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the monolactam antibiotic nocardicin A. It catalyses the epimerization of the amino group at position 9' from (S)- configuration to (R)-. The enzyme can act on both isonocardicin A and isonocardicin C, but the in vivo substrate appears to be the latter [3].
History
EC 5.1.1.14 created 1992, modified 2016
Pathway
ec00261  Monobactam biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K19105  nocardicin-A epimerase
Genes
AMI: Amir_4594
Taxonomy
Reference
1
  Authors
Wilson, B.A., Bantia, S., Salituro, G.M., Reeve, A.M. and Townsend, C.A.
  Title
Cell-free biosynthesis of nocardicin A from nocardicin E and S-adenosylmethionine.
  Journal
J Am Chem Soc 110:8238-8239 (1988)
Reference
2  [PMID:15252031]
  Authors
Kelly WL, Townsend CA.
  Title
Mutational analysis and characterization of nocardicin C-9' epimerase.
  Journal
J Biol Chem 279:38220-7 (2004)
DOI:10.1074/jbc.M405450200
Reference
3  [PMID:15629944]
  Authors
Kelly WL, Townsend CA.
  Title
Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.
  Journal
J Bacteriol 187:739-46 (2005)
DOI:10.1128/JB.187.2.739-746.2005
Other DBs
ExplorEnz - The Enzyme Database: 5.1.1.14
IUBMB Enzyme Nomenclature: 5.1.1.14
ExPASy - ENZYME nomenclature database: 5.1.1.14
BRENDA, the Enzyme Database: 5.1.1.14
CAS: 118246-75-6

KEGG   REACTION: R03073Help
Entry
R03073                      Reaction                               

Name
nocardicin-A epimerase;
isonocardicin-A epimerase
Definition
Isonocardicin A <=> Nocardicin A
Equation
Reaction class
RC00302  C00927_C01941
Enzyme
Pathway
rn00261  Monobactam biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00736  Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A
Orthology
K19105  nocardicin-A epimerase [EC:5.1.1.14]
Other DBs
RHEA: 22795

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