KEGG   ORTHOLOGY: K19290
Entry
K19290                      KO                                     

Name
alg8
Definition
mannuronan synthase [EC:2.4.1.33]
Pathway
ko00051  Fructose and mannose metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00051 Fructose and mannose metabolism
    K19290  alg8; mannuronan synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01003 Glycosyltransferases
    K19290  alg8; mannuronan synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.33  mannuronan synthase
     K19290  alg8; mannuronan synthase
Glycosyltransferases [BR:ko01003]
 Polysaccharide
  Bacterial polysaccharide
   K19290  alg8; mannuronan synthase
Other DBs
RN: R08692
COG: COG1215
TC: 4.D.1.1.7 4.D.1.1.9
CAZy: GT2
Genes
PAE: PA3541(alg8)
PAEV: N297_3664
PAEI: N296_3664
PAU: PA14_18565(alg8)
PAP: PSPA7_1604
PAG: PLES_14921(alg8)
PAF: PAM18_1445(alg8)
PNC: NCGM2_4666(alg8)
PAEB: NCGM1900_2743(alg8)
PDK: PADK2_06660
PAEP: PA1S_07715
PAEM: U769_07190
PAEL: T223_07430
PAEG: AI22_26190
PAEC: M802_3661
PAEO: M801_3529
PMK: MDS_1081
PRE: PCA10_11870(alg8) PCA10_38220(alg8)
PPU: PP_1287(alg8)
PPF: Pput_4438
PPT: PPS_4404
PPI: YSA_03094
PPX: T1E_3167
PPUH: B479_22155
PPUT: L483_27240
PPUN: PP4_07990(alg8)
PPUD: DW66_4795
PMON: X969_21690
PMOT: X970_21325
PSB: Psyr_1062
PSYR: N018_20360
PVD: CFBP1590__4278(alg8)
PFL: PFL_1023
PPRC: PFLCHA0_c10430(alg2)
PPRO: PPC_1061
PFS: PFLU_0989
PFE: PSF113_4753(alg8)
PFB: VO64_4070
PMAN: OU5_2448
PFW: PF1751_v1c09470(alg8)
PEN: PSEEN4536(alg8)
PPUU: PputUW4_04472(alg8)
PKC: PKB_0967(alg8)
PSES: PSCI_0722
PSEM: TO66_05205
PSEC: CCOS191_0911(alg8a) CCOS191_2028(alg8b)
PSOS: POS17_1029
PANR: A7J50_0991
PSIL: PMA3_24590
AVN: Avin_10960(alg8)
AVL: AvCA_10960(alg8)
AVD: AvCA6_10960(alg8)
ACX: Achr_31190(alg8)
PSPO: PSPO_a3133
ABO: ABO_0385(alg8)
TAU: Tola_1408
ENM: EBS_1359
NIS: NIS_0190
RHL: LPU83_pLPU83d0170(alg8-1) LPU83_pLPU83d1237(alg8-2)
MMED: Mame_02305
DSH: Dshi_1898
MALG: MALG_02777
SECH: B18_00670
NSH: GXM_00033
AAE: aq_1684(alg)
 » show all
Reference
PMID:8294014
  Authors
Maharaj R, May TB, Wang SK, Chakrabarty AM
  Title
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
  Journal
Gene 136:267-9 (1993)
DOI:10.1016/0378-1119(93)90477-K
  Sequence
[pae:PA3541]
Reference
  Authors
Remminghorst U, Rehm BH
  Title
In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa.
  Journal
Appl Environ Microbiol 72:298-305 (2006)
DOI:10.1128/AEM.72.1.298-305.2006

KEGG   ORTHOLOGY: K19291
Entry
K19291                      KO                                     

Name
alg44
Definition
mannuronan synthase [EC:2.4.1.33]
Pathway
ko00051  Fructose and mannose metabolism
ko01100  Metabolic pathways
ko02025  Biofilm formation - Pseudomonas aeruginosa
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00051 Fructose and mannose metabolism
    K19291  alg44; mannuronan synthase
 09140 Cellular Processes
  09145 Cellular community - prokaryotes
   02025 Biofilm formation - Pseudomonas aeruginosa
    K19291  alg44; mannuronan synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.33  mannuronan synthase
     K19291  alg44; mannuronan synthase
Other DBs
RN: R08692
COG: COG5581
Genes
PAE: PA3542(alg44)
PAEV: N297_3665(alg44)
PAEI: N296_3665(alg44)
PAU: PA14_18550(alg44)
PAP: PSPA7_1603
PAG: PLES_14911(alg44)
PAF: PAM18_1444(alg44)
PNC: NCGM2_4667(alg44)
PAEB: NCGM1900_2742(alg44)
PDK: PADK2_06655
PAEP: PA1S_07710
PAEM: U769_07185
PAEL: T223_07425
PAEU: BN889_03912(alg44_1)
PAEG: AI22_26195
PAEC: M802_3662(alg44)
PAEO: M801_3530(alg44)
PMK: MDS_1080
PRE: PCA10_11860(alg44) PCA10_38210(alg44)
PPU: PP_1286(alg44)
PPF: Pput_4439
PPT: PPS_4405
PPI: YSA_03096
PPX: T1E_3166
PPUH: B479_22160
PPUT: L483_27245
PPUN: PP4_07980(alg44)
PPUD: DW66_4796
PMON: X969_21695
PMOT: X970_21330
PSB: Psyr_1061
PSYR: N018_20365
PVD: CFBP1590__4279(alg44)
PFL: PFL_1022(alg44)
PPRC: PFLCHA0_c10420(alg1)
PPRO: PPC_1060
PFS: PFLU_0988
PFE: PSF113_4754(alg44)
PFC: PflA506_0968(alg44)
PFB: VO64_4069
PMAN: OU5_2449
PFW: PF1751_v1c09460(alg44)
PEN: PSEEN3455 PSEEN4537(alg44)
PPUU: PputUW4_04473(alg44)
PKC: PKB_0966(alg44)
PSES: PSCI_0723
PSEM: TO66_05200
PSOS: POS17_1028
PANR: A7J50_0990
PSIL: PMA3_24595
AVN: Avin_10950(alg44)
AVL: AvCA_10950(alg44)
AVD: AvCA6_10950(alg44)
ACX: Achr_31200(alg44)
ABO: ABO_0386(alg44)
TAU: Tola_1409
NIS: NIS_0189
SECH: B18_00675
BBA: Bd2717
BBAT: Bdt_2636
BBAC: EP01_09740
 » show all
Reference
PMID:8294014
  Authors
Maharaj R, May TB, Wang SK, Chakrabarty AM
  Title
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
  Journal
Gene 136:267-9 (1993)
DOI:10.1016/0378-1119(93)90477-K
  Sequence
[pae:PA3542]
Reference
  Authors
Remminghorst U, Rehm BH
  Title
Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa.
  Journal
FEBS Lett 580:3883-8 (2006)
DOI:10.1016/j.febslet.2006.05.077

KEGG   ENZYME: 2.4.1.33
Entry
EC 2.4.1.33                 Enzyme                                 

Name
mannuronan synthase;
mannuronosyl transferase;
alginate synthase (incorrect);
alg8 (gene name);
alg44 (gene name);
GDP-D-mannuronate:alginate D-mannuronyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-alpha-D-mannuronate:mannuronan D-mannuronatetransferase
Reaction(IUBMB)
GDP-alpha-D-mannuronate + [(1->4)-beta-D-mannuronosyl]n = GDP + [(1->4)-beta-D-mannuronosyl]n+1 [RN:R08692]
Reaction(KEGG)
R08692;
(other) R03129
Substrate
GDP-alpha-D-mannuronate;
[(1->4)-beta-D-mannuronosyl]n
Product
GDP [CPD:C00035];
[(1->4)-beta-D-mannuronosyl]n+1
Comment
The enzyme catalyses the polymerization of beta-D-mannuronate residues into a mannuronan polymer, an intermediate in the biosynthesis of alginate. It is found in brown algae and in alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
History
EC 2.4.1.33 created 1972, modified 2015
Pathway
ec00051  Fructose and mannose metabolism
ec01100  Metabolic pathways
Orthology
K19290  mannuronan synthase
K19291  mannuronan synthase
Genes
PAE: PA3541(alg8) PA3542(alg44)
PAEV: N297_3664 N297_3665(alg44)
PAEI: N296_3664 N296_3665(alg44)
PAU: PA14_18550(alg44) PA14_18565(alg8)
PAP: PSPA7_1603 PSPA7_1604
PAG: PLES_14911(alg44) PLES_14921(alg8)
PAF: PAM18_1444(alg44) PAM18_1445(alg8)
PNC: NCGM2_4666(alg8) NCGM2_4667(alg44)
PAEB: NCGM1900_2742(alg44) NCGM1900_2743(alg8)
PDK: PADK2_06655 PADK2_06660
PAEU: BN889_03911 BN889_03912(alg44_1)
PAEC: M802_3661 M802_3662(alg44)
PAEO: M801_3529 M801_3530(alg44)
PRE: PCA10_11860(alg44) PCA10_11870(alg8) PCA10_38210(alg44) PCA10_38220(alg8)
PPU: PP_1286(alg44) PP_1287(alg8)
PPUN: PP4_07980(alg44) PP4_07990(alg8)
PVD: CFBP1590__4278(alg8) CFBP1590__4279(alg44)
PFL: PFL_1022(alg44) PFL_1023
PPRC: PFLCHA0_c10420(alg1) PFLCHA0_c10430(alg2)
PFE: PSF113_4753(alg8) PSF113_4754(alg44)
PFW: PF1751_v1c09460(alg44) PF1751_v1c09470(alg8)
PEN: PSEEN3455 PSEEN4536(alg8) PSEEN4537(alg44)
PPUU: PputUW4_04472(alg8) PputUW4_04473(alg44)
PKC: PKB_0966(alg44) PKB_0967(alg8)
AVN: Avin_10950(alg44) Avin_10960(alg8)
AVL: AvCA_10950(alg44) AvCA_10960(alg8)
AVD: AvCA6_10950(alg44) AvCA6_10960(alg8)
ACX: Achr_31190(alg8) Achr_31200(alg44)
PSPO: PSPO_a3133
ABO: ABO_0385(alg8) ABO_0386(alg44)
ENM: EBS_1359
MMED: Mame_02305
DSH: Dshi_1898
MALG: MALG_02777
BBA: Bd2717
BBAT: Bdt_2636
BBAC: EP01_09740
NSH: GXM_00033
AAE: aq_1684(alg)
 » show all
Reference
1  [PMID:5954796]
  Authors
Lin TY, Hassid WZ.
  Title
Pathway of algnic acid synthesis in the marine brown alga, Fucus gardneri Silva.
  Journal
J Biol Chem 241:5284-97 (1966)
Reference
2  [PMID:16391057]
  Authors
Remminghorst U, Rehm BH
  Title
In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa.
  Journal
Appl Environ Microbiol 72:298-305 (2006)
DOI:10.1128/AEM.72.1.298-305.2006
  Sequence
[pae:PA3541]
Reference
3  [PMID:18524915]
  Authors
Oglesby LL, Jain S, Ohman DE
  Title
Membrane topology and roles of Pseudomonas aeruginosa Alg8 and Alg44 in alginate  polymerization.
  Journal
Microbiology 154:1605-15 (2008)
DOI:10.1099/mic.0.2007/015305-0
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.33
IUBMB Enzyme Nomenclature: 2.4.1.33
ExPASy - ENZYME nomenclature database: 2.4.1.33
BRENDA, the Enzyme Database: 2.4.1.33
CAS: 37257-31-1

KEGG   REACTION: R08692
Entry
R08692                      Reaction                               

Name
GDP-D-mannuronate:Poly(beta-D-mannuronate) beta-D-mannuronyltransferase
Definition
GDP-D-mannuronate + (beta-D-Mannuronate)n <=> (beta-D-Mannuronate)n + GDP
Equation
Reaction class
RC00005  C00035_C00976
Enzyme
Pathway
rn00051  Fructose and mannose metabolism
rn01100  Metabolic pathways
Orthology
K19290  mannuronan synthase [EC:2.4.1.33]
K19291  mannuronan synthase [EC:2.4.1.33]
Other DBs
RHEA: 46879

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