KEGG   ORTHOLOGY: K19669
Entry
K19669                      KO                                     

Name
pphA
Definition
phosphonopyruvate hydrolase [EC:3.11.1.3]
Pathway
ko00440  Phosphonate and phosphinate metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00440 Phosphonate and phosphinate metabolism
    K19669  pphA; phosphonopyruvate hydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.11  Acting on carbon-phosphorus bonds
   3.11.1  Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
    3.11.1.3  phosphonopyruvate hydrolase
     K19669  pphA; phosphonopyruvate hydrolase
Other DBs
RN: R08200
GO: 0033978
Genes
VPD: VAPA_2c11770(palA)
AG: AAO24736(pphA)
Reference
  Authors
Kulakova AN, Wisdom GB, Kulakov LA, Quinn JP
  Title
The purification and characterization of phosphonopyruvate hydrolase, a novel carbon-phosphorus bond cleavage enzyme from Variovorax sp Pal2.
  Journal
J Biol Chem 278:23426-31 (2003)
DOI:10.1074/jbc.M301871200
  Sequence

KEGG   ENZYME: 3.11.1.3
Entry
EC 3.11.1.3                 Enzyme                                 

Name
phosphonopyruvate hydrolase;
PPH
Class
Hydrolases;
Acting on carbon-phosphorus bonds;
Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
Reaction(IUBMB)
3-phosphonopyruvate + H2O = pyruvate + phosphate [RN:R08200]
Reaction(KEGG)
R08200
Substrate
3-phosphonopyruvate [CPD:C02798];
H2O [CPD:C00001]
Product
pyruvate [CPD:C00022];
phosphate [CPD:C00009]
Comment
Highly specific for phosphonopyruvate as substrate [2]. The reaction is not inhibited by phosphate but is inhibited by the phosphonates phosphonoformic acid, hydroxymethylphosphonic acid and 3-phosphonopropanoic acid [2]. The enzyme is activated by the divalent cations Co2+, Mg2+ and Mn2+. This enzyme is a member of the phosphoenolpyruvate mutase/isocitrate lyase superfamily [3].
History
EC 3.11.1.3 created 2007
Pathway
ec00440  Phosphonate and phosphinate metabolism
ec01100  Metabolic pathways
Orthology
K19669  phosphonopyruvate hydrolase
Genes
VPD: VAPA_2c11770(palA)
Reference
1  [PMID:10648102]
  Authors
Ternan NG, Hamilton JT, Quinn JP.
  Title
Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6.
  Journal
Arch Microbiol 173:35-41 (2000)
DOI:10.1007/s002030050005
Reference
2  [PMID:12697754]
  Authors
Kulakova AN, Wisdom GB, Kulakov LA, Quinn JP.
  Title
The purification and characterization of phosphonopyruvate hydrolase, a novel carbon-phosphorus bond cleavage enzyme from Variovorax sp Pal2.
  Journal
J Biol Chem 278:23426-31 (2003)
DOI:10.1074/jbc.M301871200
  Sequence
Reference
3  [PMID:16981709]
  Authors
Chen CC, Han Y, Niu W, Kulakova AN, Howard A, Quinn JP, Dunaway-Mariano D, Herzberg O.
  Title
Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily.
  Journal
Biochemistry 45:11491-504 (2006)
DOI:10.1021/bi061208l
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.11.1.3
IUBMB Enzyme Nomenclature: 3.11.1.3
ExPASy - ENZYME nomenclature database: 3.11.1.3
BRENDA, the Enzyme Database: 3.11.1.3

KEGG   REACTION: R08200
Entry
R08200                      Reaction                               

Definition
3-Phosphonopyruvate + H2O <=> Pyruvate + Orthophosphate
Equation
Reaction class
RC02147  C00022_C02798
Enzyme
Pathway
rn00440  Phosphonate and phosphinate metabolism
rn01100  Metabolic pathways
Orthology
K19669  phosphonopyruvate hydrolase [EC:3.11.1.3]
Other DBs
RHEA: 16676

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