KEGG   ORTHOLOGY: K19742
Entry
K19742                      KO                                     

Name
lhpI
Definition
1-pyrroline-2-carboxylate reductase [NAD(P)H] [EC:1.5.1.49]
Pathway
ko00330  Arginine and proline metabolism
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00330 Arginine and proline metabolism
    K19742  lhpI; 1-pyrroline-2-carboxylate reductase [NAD(P)H]
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.49  1-pyrroline-2-carboxylate reductase [NAD(P)H]
     K19742  lhpI; 1-pyrroline-2-carboxylate reductase [NAD(P)H]
Other DBs
RN: R01246 R01249
COG: COG2423
Genes
VPA: VP1333
VPB: VPBB_1257
VPK: M636_15145
VPF: M634_08575
VPH: VPUCM_1890
VAG: N646_0368
VEX: VEA_003670
VDB: AL552_14870
VHR: AL538_01790
VOW: A9237_01110
SAZ: Sama_2610
SLO: Shew_2955
SSE: Ssed_3503
SPL: Spea_3170
SHL: Shal_3252
SWD: Swoo_3726
SWP: swp_3859
SVO: SVI_3429
SPSW: Sps_04403
CPS: CPS_1455
PTN: PTRA_b0312(ocd)
PSEO: OM33_02120
PSPO: PSPO_a3016(ocd)
PTU: PTUN_a0321(ocd)
PIN: Ping_2540
SAGA: M5M_03795
 » show all
Reference
  Authors
Watanabe S, Tanimoto Y, Yamauchi S, Tozawa Y, Sawayama S, Watanabe Y
  Title
Identification and characterization of trans-3-hydroxy-l-proline dehydratase and Delta(1)-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria.
  Journal
FEBS Open Bio 4:240-50 (2014)
DOI:10.1016/j.fob.2014.02.010
  Sequence
[cps:CPS_1455]

KEGG   ENZYME: 1.5.1.49
Entry
EC 1.5.1.49                 Enzyme                                 

Name
1-pyrroline-2-carboxylate reductase [NAD(P)H]
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
L-proline:NAD(P)+ 2-oxidoreductase
Reaction(IUBMB)
L-proline + NAD(P)+ = 1-pyrroline-2-carboxylate + NAD(P)H + H+ [RN:R01246 R01249]
Reaction(KEGG)
R01246 R01249
Substrate
L-proline [CPD:C00148];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
1-pyrroline-2-carboxylate [CPD:C03564];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme from the bacterium Colwellia psychrerythraea is involved in trans-3-hydroxy-L-proline metabolism. In contrast to EC 1.5.1.1, 1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H], which shows similar activity with 1-piperideine-2-carboxylate and 1-pyrroline-2-carboxylate, this enzyme is specific for the latter. While the enzyme is active with both NADH and NADPH, activity is higher with NADPH.
History
EC 1.5.1.49 created 2015
Pathway
ec00330  Arginine and proline metabolism
Orthology
K19742  1-pyrroline-2-carboxylate reductase [NAD(P)H]
Genes
VPA: VP1333
VPB: VPBB_1257
VPK: M636_15145
VPF: M634_08575
VPH: VPUCM_1890
VAG: N646_0368
VEX: VEA_003670
VDB: AL552_14870
VHR: AL538_01790
VOW: A9237_01110
SAZ: Sama_2610
SLO: Shew_2955
SSE: Ssed_3503
SPL: Spea_3170
SHL: Shal_3252
SWD: Swoo_3726
SWP: swp_3859
SVO: SVI_3429
SPSW: Sps_04403
CPS: CPS_1455
PTN: PTRA_b0312(ocd)
PSEO: OM33_02120
PSPO: PSPO_a3016(ocd)
PTU: PTUN_a0321(ocd)
PIN: Ping_2540
SAGA: M5M_03795
 » show all
Reference
1  [PMID:24649405]
  Authors
Watanabe S, Tanimoto Y, Yamauchi S, Tozawa Y, Sawayama S, Watanabe Y
  Title
Identification and characterization of trans-3-hydroxy-l-proline dehydratase and Delta(1)-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria.
  Journal
FEBS Open Bio 4:240-50 (2014)
DOI:10.1016/j.fob.2014.02.010
  Sequence
[cps:CPS_1455]
Other DBs
ExplorEnz - The Enzyme Database: 1.5.1.49
IUBMB Enzyme Nomenclature: 1.5.1.49
ExPASy - ENZYME nomenclature database: 1.5.1.49
BRENDA, the Enzyme Database: 1.5.1.49

KEGG   REACTION: R01246
Entry
R01246                      Reaction                               

Name
L-proline:NAD+ 2-oxidoreductase
Definition
L-Proline + NAD+ <=> 1-Pyrroline-2-carboxylate + NADH + H+
Equation
Comment
NADP+ (see R01249)
Reaction class
RC00001  C00003_C00004
RC00135  C00148_C03564
Enzyme
1.5.1.1         1.5.1.49
Pathway
rn00330  Arginine and proline metabolism
Orthology
K19742  1-pyrroline-2-carboxylate reductase [NAD(P)H] [EC:1.5.1.49]
K19743  1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H] [EC:1.5.1.1]
Other DBs
RHEA: 20324

KEGG   REACTION: R01249
Entry
R01249                      Reaction                               

Name
L-proline:NADP+ 2-oxidoreductase
Definition
L-Proline + NADP+ <=> 1-Pyrroline-2-carboxylate + NADPH + H+
Equation
Comment
NAD+ (see R01246)
Reaction class
RC00001  C00005_C00006
RC00135  C00148_C03564
Enzyme
1.5.1.1         1.5.1.21        1.5.1.49
Pathway
rn00330  Arginine and proline metabolism
Orthology
K19742  1-pyrroline-2-carboxylate reductase [NAD(P)H] [EC:1.5.1.49]
K19743  1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H] [EC:1.5.1.1]
Other DBs
RHEA: 20320

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