KEGG   ORTHOLOGY: K19823
Entry
K19823                      KO                                     

Name
NAO
Definition
nitroalkane oxidase [EC:1.7.3.1]
Pathway
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00910 Nitrogen metabolism
    K19823  NAO; nitroalkane oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.7  Acting on other nitrogenous compounds as donors
   1.7.3  With oxygen as acceptor
    1.7.3.1  nitroalkane oxidase
     K19823  NAO; nitroalkane oxidase
Other DBs
RN: R10388 R10389
GO: 0052664
Genes
PAN: PODANSg2158 PODANSg4289
TMN: UCRPA7_3412
FGR: FGSG_02379
FPU: FPSE_06845
FVR: FVEG_05969
FOX: FOXG_08703
NHE: NECHADRAFT_81017
CFJ: CFIO01_03827
ELA: UCREL1_3529
PFY: PFICI_01984
ANI: AN9162.2
PNO: SNOG_15929(SNOG_15928)
AVI: Avi_7637
BBT: BBta_3103
PSIM: KR76_25720
SEN: SACE_5017(acdA)
PSEH: XF36_21325
AG: AAL57485(NAO)
 » show all
Reference
  Authors
Daubner SC, Gadda G, Valley MP, Fitzpatrick PF
  Title
Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily.
  Journal
Proc Natl Acad Sci U S A 99:2702-7 (2002)
DOI:10.1073/pnas.052527799
  Sequence

KEGG   ENZYME: 1.7.3.1
Entry
EC 1.7.3.1                  Enzyme                                 

Name
nitroalkane oxidase;
nitroethane oxidase;
NAO;
nitroethane:oxygen oxidoreductase
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
Sysname
nitroalkane:oxygen oxidoreductase
Reaction(IUBMB)
a nitroalkane + H2O + O2 = an aldehyde or ketone + nitrite + H2O2 [RN:R10388 R10389]
Reaction(KEGG)
R10388 > R00799;
R10389
Substrate
nitroalkane [CPD:C06058];
H2O [CPD:C00001];
O2 [CPD:C00007]
Product
aldehyde [CPD:C00071];
ketone [CPD:C01450];
nitrite [CPD:C00088];
H2O2 [CPD:C00027]
Comment
Has an absolute requirement for FAD [4]. While nitroethane may be the physiological substrate [2], the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols [4]. Differs from EC 1.13.11.16, nitronate monooxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates [4].
History
EC 1.7.3.1 created 1961, modified 2006, modified 2009
Pathway
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
Orthology
K19823  nitroalkane oxidase
Genes
PAN: PODANSg2158 PODANSg4289
TMN: UCRPA7_3412
FGR: FGSG_02379
FPU: FPSE_06845
FVR: FVEG_05969
FOX: FOXG_08703
NHE: NECHADRAFT_81017
CFJ: CFIO01_03827
ELA: UCREL1_3529
PFY: PFICI_01984
ANI: AN9162.2
PNO: SNOG_15929(SNOG_15928)
AVI: Avi_7637
BBT: BBta_3103
PSIM: KR76_25720
SEN: SACE_5017(acdA)
PSEH: XF36_21325
 » show all
Reference
1  [PMID:14907722]
  Authors
LITTLE HN.
  Title
Oxidation of nitroethane by extracts from Neurospora.
  Journal
J Biol Chem 193:347-58 (1951)
Reference
2  [PMID:22538]
  Authors
Kido T, Hashizume K, Soda K.
  Title
Purification and properties of nitroalkane oxidase from Fusarium oxysporum.
  Journal
J Bacteriol 133:53-8 (1978)
DOI:10.1128/JB.133.1.53-58.1978
Reference
3  [PMID:11867731]
  Authors
Daubner SC, Gadda G, Valley MP, Fitzpatrick PF.
  Title
Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily.
  Journal
Proc Natl Acad Sci U S A 99:2702-7 (2002)
DOI:10.1073/pnas.052527799
  Sequence
Reference
4  [PMID:15581574]
  Authors
Fitzpatrick PF, Orville AM, Nagpal A, Valley MP.
  Title
Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase.
  Journal
Arch Biochem Biophys 433:157-65 (2005)
DOI:10.1016/j.abb.2004.08.021
Reference
5  [PMID:15713081]
  Authors
Valley MP, Tichy SE, Fitzpatrick PF.
  Title
Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase.
  Journal
J Am Chem Soc 127:2062-6 (2005)
DOI:10.1021/ja043542f
Other DBs
ExplorEnz - The Enzyme Database: 1.7.3.1
IUBMB Enzyme Nomenclature: 1.7.3.1
ExPASy - ENZYME nomenclature database: 1.7.3.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.7.3.1
BRENDA, the Enzyme Database: 1.7.3.1
CAS: 9029-36-1 65802-82-6

KEGG   REACTION: R10388
Entry
R10388                      Reaction                               

Name
nitroalkane:oxygen oxidoreductase
Definition
Nitroalkane + H2O + Oxygen <=> Aldehyde + Nitrite + Hydrogen peroxide
Equation
Comment
general reaction
Reaction class
RC02755  C00007_C00027
RC03154  C00088_C06058
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
Orthology
K19823  nitroalkane oxidase [EC:1.7.3.1]
Other DBs
RHEA: 20979

KEGG   REACTION: R10389
Entry
R10389                      Reaction                               

Name
nitroalkane:oxygen oxidoreductase
Definition
Nitroalkane + H2O + Oxygen <=> Ketone + Nitrite + Hydrogen peroxide
Equation
Comment
general reaction
Reaction class
RC02755  C00007_C00027
RC03154  C00088_C06058
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
Orthology
K19823  nitroalkane oxidase [EC:1.7.3.1]
Other DBs
RHEA: 26493

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