Entry |
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Name |
dichloroarcyriaflavin A synthase
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Class |
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
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Sysname |
dichlorochromopyrrolate,NADH:oxygen 2,5-oxidoreductase (dichloroarcyriaflavin A-forming)
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Reaction(IUBMB) |
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ = dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ [RN: R09567]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
The conversion of dichlorochromopyrrolate to dichloroarcyriaflavin A is a complex process that involves two enzyme components. RebP is an NAD-dependent cytochrome P-450 oxygenase that performs an aryl-aryl bond formation yielding the six-ring indolocarbazole scaffold [1]. Along with RebC, a flavin-dependent hydroxylase, it also catalyses the oxidative decarboxylation of both carboxyl groups. The presence of RebC ensures that the only product is the rebeccamycin aglycone dichloroarcyriaflavin A [2]. The enzymes are similar, but not identical, to StaP and StaC, which are involved in the synthesis of staurosporine [3].
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History |
EC 1.13.12.17 created 2010
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Pathway |
ec01130 | Biosynthesis of antibiotics |
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Orthology |
K19886 | flavin-dependent monooxygenase RebC |
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Reference |
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Authors |
Makino M, Sugimoto H, Shiro Y, Asamizu S, Onaka H, Nagano S |
Title |
Crystal structures and catalytic mechanism of cytochrome P450 StaP that produces the indolocarbazole skeleton. |
Journal |
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Reference |
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Authors |
Howard-Jones AR, Walsh CT |
Title |
Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid. |
Journal |
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Reference |
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Authors |
Sanchez C, Zhu L, Brana AF, Salas AP, Rohr J, Mendez C, Salas JA |
Title |
Combinatorial biosynthesis of antitumor indolocarbazole compounds. |
Journal |
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Other DBs |
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