Entry |
|
Name |
4'-demethylrebeccamycin synthase;
arcyriaflavin A N-glycosyltransferase;
RebG
|
Class |
Lyases;
Carbon-nitrogen lyases;
Amine-lyases
|
Sysname |
4'-demethylrebeccamycin D-glucose-lyase
|
Reaction(IUBMB) |
4'-O-demethylrebeccamycin + H2O = dichloro-arcyriaflavin A + beta-D-glucose [RN: R09568]
|
Reaction(KEGG) |
|
Substrate |
|
Product |
dichloroarcyriaflavin A [CPD: C19699];
beta-D-glucose [CPD: C00221]
|
Comment |
This enzyme catalyses a step in the biosynthesis of rebeccamycin, an indolocarbazole alkaloid produced by the bacterium Lechevalieria aerocolonigenes. The enzyme is a glycosylase, and acts in the reverse direction to that shown. It has a wide substrate range, and was shown to glycosylate several substrates, including the staurosporine aglycone, EJG-III-108A, J-104303, 6-N-methyl-arcyriaflavin C and indolo-[2,3-a]-carbazole [1,2].
|
History |
EC 4.3.3.5 created 2010
|
Pathway |
ec01110 | Biosynthesis of secondary metabolites |
|
Orthology |
K19888 | 4'-demethylrebeccamycin synthase |
|
Reference |
|
Authors |
Ohuchi T, Ikeda-Araki A, Watanabe-Sakamoto A, Kojiri K, Nagashima M, Okanishi M, Suda H |
Title |
Cloning and expression of a gene encoding N-glycosyltransferase (ngt) from Saccarothrix aerocolonigenes ATCC39243. |
Journal |
|
Reference |
|
Authors |
Zhang C, Albermann C, Fu X, Peters NR, Chisholm JD, Zhang G, Gilbert EJ, Wang PG, Van Vranken DL, Thorson JS |
Title |
RebG- and RebM-catalyzed indolocarbazole diversification. |
Journal |
|
Sequence |
|
Other DBs |
ExplorEnz - The Enzyme Database: | 4.3.3.5 |
ExPASy - ENZYME nomenclature database: | 4.3.3.5 |
BRENDA, the Enzyme Database: | 4.3.3.5 |
|