| Entry |
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| Name |
4'-demethylrebeccamycin synthase;
arcyriaflavin A N-glycosyltransferase;
RebG
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| Class |
Lyases;
Carbon-nitrogen lyases;
Amine-lyases
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| Sysname |
4'-demethylrebeccamycin D-glucose-lyase
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| Reaction(IUBMB) |
4'-O-demethylrebeccamycin + H2O = dichloro-arcyriaflavin A + beta-D-glucose [RN: R09568]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
dichloroarcyriaflavin A [CPD: C19699];
beta-D-glucose [CPD: C00221]
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| Comment |
This enzyme catalyses a step in the biosynthesis of rebeccamycin, an indolocarbazole alkaloid produced by the bacterium Lechevalieria aerocolonigenes. The enzyme is a glycosylase, and acts in the reverse direction to that shown. It has a wide substrate range, and was shown to glycosylate several substrates, including the staurosporine aglycone, EJG-III-108A, J-104303, 6-N-methyl-arcyriaflavin C and indolo-[2,3-a]-carbazole [1,2].
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| History |
EC 4.3.3.5 created 2010
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| Pathway |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K19888 | 4'-demethylrebeccamycin synthase |
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| Reference |
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| Authors |
Ohuchi T, Ikeda-Araki A, Watanabe-Sakamoto A, Kojiri K, Nagashima M, Okanishi M, Suda H |
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| Title |
Cloning and expression of a gene encoding N-glycosyltransferase (ngt) from Saccarothrix aerocolonigenes ATCC39243. |
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| Journal |
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| Reference |
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| Authors |
Zhang C, Albermann C, Fu X, Peters NR, Chisholm JD, Zhang G, Gilbert EJ, Wang PG, Van Vranken DL, Thorson JS |
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| Title |
RebG- and RebM-catalyzed indolocarbazole diversification. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 4.3.3.5 |
| ExPASy - ENZYME nomenclature database: | 4.3.3.5 |
| BRENDA, the Enzyme Database: | 4.3.3.5 |
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