KEGG   ORTHOLOGY: K20089Help
Entry
K20089                      KO                                     

Name
vioD
Definition
protodeoxyviolaceinate monooxygenase [EC:1.14.13.217]
Pathway
ko00404  Staurosporine biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
ko02024  Quorum sensing
Module
M00808  Violacein biosynthesis, tryptophan => violacein
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00404 Staurosporine biosynthesis
    K20089  vioD; protodeoxyviolaceinate monooxygenase
 09140 Cellular Processes
  09145 Cellular community - prokaryotes
   02024 Quorum sensing
    K20089  vioD; protodeoxyviolaceinate monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.217  protodeoxyviolaceinate monooxygenase
     K20089  vioD; protodeoxyviolaceinate monooxygenase
BRITE hierarchy
Other DBs
RN: R11134 R11158
COG: COG0654
Genes
CVI: CV_3271(vioD)
CVC: BKX93_07100
CHRI: DK842_01225
JAG: GJA_1083(vioD)
JAZ: YQ44_10555
JAL: BZG29_16960
MNR: ACZ75_19205
MASS: CR152_21620
RIV: Riv7116_3967
TaxonomyKoalaUniProt
Reference
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
  Sequence
[cvi:CV_3271]

KEGG   ENZYME: 1.14.13.217Help
Entry
EC 1.14.13.217              Enzyme                                 

Name
protodeoxyviolaceinate monooxygenase;
vioD (gene name);
protoviolaceinate synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
protodeoxyviolaceinate,NAD(P)H:O2 oxidoreductase
Reaction(IUBMB)
protodeoxyviolaceinate + NAD(P)H + O2 = protoviolaceinate + NAD(P)+ + H2O [RN:R11134 R11158]
Reaction(KEGG)
Substrate
protodeoxyviolaceinate [CPD:C21131];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007]
Product
protoviolaceinate [CPD:C21134];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The product, protoviolaceinate, can be acted upon by EC 1.14.13.224, violacein synthase, leading to violacein production. However, it is very labile, and in the presence of oxygen can undergo non-enzymic autooxidation to the shunt product proviolacein.
History
EC 1.14.13.217 created 2016, modified 2016
Pathway
ec00404  Staurosporine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K20089  protodeoxyviolaceinate monooxygenase
Genes
CVI: CV_3271(vioD)
CVC: BKX93_07100
CHRI: DK842_01225
JAG: GJA_1083(vioD)
JAZ: YQ44_10555
JAL: BZG29_16960
MNR: ACZ75_19205
MASS: CR152_21620
RIV: Riv7116_3967
Taxonomy
Reference
1  [PMID:17176066]
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
Reference
2  [PMID:17925955]
  Authors
Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T.
  Title
Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.
  Journal
Chem Commun (Camb) 4140-2 (2007)
DOI:10.1039/b705358d
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.217
IUBMB Enzyme Nomenclature: 1.14.13.217
ExPASy - ENZYME nomenclature database: 1.14.13.217
BRENDA, the Enzyme Database: 1.14.13.217

KEGG   REACTION: R11134Help
Entry
R11134                      Reaction                               

Name
protodeoxyviolaceinate,NADPH:O2 oxidoreductase
Definition
Protodeoxyviolaceinic acid + NADPH + Oxygen + H+ <=> Protoviolaceinic acid + NADP+ + H2O
Equation
Comment
NADH (see R11158)
Reaction class
RC00001  C00005_C00006
RC00490  C21131_C21134
Enzyme
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00808  Violacein biosynthesis, tryptophan => violacein
Orthology
K20089  protodeoxyviolaceinate monooxygenase [EC:1.14.13.217]
Other DBs
RHEA: 49127

KEGG   REACTION: R11158Help
Entry
R11158                      Reaction                               

Name
protodeoxyviolaceinate,NADH:O2 oxidoreductase
Definition
Protodeoxyviolaceinic acid + NADH + H+ + Oxygen <=> Protoviolaceinic acid + NAD+ + H2O
Equation
Comment
NADPH (see R11134)
Reaction class
RC00001  C00003_C00004
RC00490  C21131_C21134
Enzyme
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Orthology
K20089  protodeoxyviolaceinate monooxygenase [EC:1.14.13.217]
Other DBs
RHEA: 49375

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