KEGG   ORTHOLOGY: K20090Help
Entry
K20090                      KO                                     

Name
vioC
Definition
violacein synthase [EC:1.14.13.224]
Pathway
ko00404  Staurosporine biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
ko02024  Quorum sensing
Module
M00808  Violacein biosynthesis, tryptophan => violacein
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00404 Staurosporine biosynthesis
    K20090  vioC; violacein synthase
 09140 Cellular Processes
  09145 Cellular community - prokaryotes
   02024 Quorum sensing
    K20090  vioC; violacein synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.224  violacein synthase
     K20090  vioC; violacein synthase
BRITE hierarchy
Other DBs
RN: R11132 R11135 R11373 R11374
COG: COG0654
Genes
CVI: CV_3272(vioC)
CVC: BKX93_07105
CHRI: DK842_01230
JAG: GJA_1082(vioC)
JAZ: YQ44_10560
JAL: BZG29_16955
MNR: ACZ75_19210
MASS: CR152_21625
MSD: MYSTI_01836
RIV: Riv7116_3968
TaxonomyKoalaUniProt
Reference
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
  Sequence
[cvi:CV_3272]

KEGG   ENZYME: 1.14.13.224Help
Entry
EC 1.14.13.224              Enzyme                                 

Name
violacein synthase;
proviolaceinate monooxygenase;
vioC (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
protoviolaceinate,NAD(P)H:O2 oxidoreductase
Reaction(IUBMB)
(1) protoviolaceinate + NAD(P)H + O2 = violaceinate + NAD(P)+ + H2O [RN:R11135 R11373];
(2) protodeoxyviolaceinate + NAD(P)H + O2 = deoxyviolaceinate + NAD(P)+ + H2O [RN:R11132 R11374]
Reaction(KEGG)
Substrate
protoviolaceinate [CPD:C21134];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007];
protodeoxyviolaceinate [CPD:C21131]
Product
violaceinate [CPD:C21135];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
deoxyviolaceinate [CPD:C21132]
Comment
The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The products, violaceinate and deoxyviolaceinate, undergo non-enzymic autooxidation into violacein and deoxyviolacein, respectively.
History
EC 1.14.13.224 created 2016
Pathway
ec00404  Staurosporine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K20090  violacein synthase
Genes
CVI: CV_3272(vioC)
CVC: BKX93_07105
CHRI: DK842_01230
JAG: GJA_1082(vioC)
JAZ: YQ44_10560
JAL: BZG29_16955
MNR: ACZ75_19210
MASS: CR152_21625
MSD: MYSTI_01836
RIV: Riv7116_3968
Taxonomy
Reference
1  [PMID:17176066]
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
Reference
2  [PMID:17925955]
  Authors
Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T.
  Title
Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.
  Journal
Chem Commun (Camb) 4140-2 (2007)
DOI:10.1039/b705358d
  Sequence
[cvi:CV_3272]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.224
IUBMB Enzyme Nomenclature: 1.14.13.224
ExPASy - ENZYME nomenclature database: 1.14.13.224
BRENDA, the Enzyme Database: 1.14.13.224

KEGG   REACTION: R11135Help
Entry
R11135                      Reaction                               

Name
protoviolaceinate,NADPH:O2 oxidoreductase
Definition
Protoviolaceinic acid + Oxygen + NADPH + H+ <=> Violaceinic acid + NADP+ + H2O
Equation
Reaction class
RC03365  C21134_C21135
Enzyme
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00808  Violacein biosynthesis, tryptophan => violacein
Orthology
K20090  violacein synthase [EC:1.14.13.224]
Other DBs
RHEA: 49123

KEGG   REACTION: R11373Help
Entry
R11373                      Reaction                               

Name
protoviolaceinate,NADH:O2 oxidoreductase
Definition
Protoviolaceinic acid + Oxygen + NADH + H+ <=> Violaceinic acid + NAD+ + H2O
Equation
Reaction class
RC03365  C21134_C21135
Enzyme
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Orthology
K20090  violacein synthase [EC:1.14.13.224]
Other DBs
RHEA: 49655

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