KEGG   ORTHOLOGY: K20158
Entry
K20158                      KO                                     

Name
pao
Definition
pseudooxynicotine oxidase [EC:1.4.3.24]
Pathway
ko00760  Nicotinate and nicotinamide metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Module
M00811  Nicotine degradation, pyrrolidine pathway, nicotine => succinate semialdehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00760 Nicotinate and nicotinamide metabolism
    K20158  pao; pseudooxynicotine oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.3  With oxygen as acceptor
    1.4.3.24  pseudooxynicotine oxidase
     K20158  pao; pseudooxynicotine oxidase
Other DBs
RN: R10103
COG: COG1231
Genes
PPT: PPS_4080
PMON: X969_19990
PMOT: X970_19625
AG: AFD54463(pao)
Reference
  Authors
Qiu J, Ma Y, Wen Y, Chen L, Wu L, Liu W
  Title
Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine.
  Journal
Appl Environ Microbiol 78:2154-60 (2012)
DOI:10.1128/AEM.07025-11
  Sequence

KEGG   ENZYME: 1.4.3.24
Entry
EC 1.4.3.24                 Enzyme                                 

Name
pseudooxynicotine oxidase
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With oxygen as acceptor
Sysname
4-(methylamino)-1-(pyridin-3-yl)butan-1-one:oxygen oxidoreductase (methylamine releasing)
Reaction(IUBMB)
4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2 = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2 [RN:R10103]
Reaction(KEGG)
R10103
Substrate
4-(methylamino)-1-(pyridin-3-yl)butan-1-one [CPD:C20361];
H2O [CPD:C00001];
O2 [CPD:C00007]
Product
4-oxo-4-(pyridin-3-yl)butanal [CPD:C19567];
methylamine [CPD:C00218];
H2O2 [CPD:C00027]
Comment
Contains one non-covalently bound FAD molecule per dimer. This enzyme, characterized from the soil bacterium Pseudomonas sp. HZN6, is involved the nicotine degradation.
History
EC 1.4.3.24 created 2012
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K20158  pseudooxynicotine oxidase
Genes
PPT: PPS_4080
PMON: X969_19990
PMOT: X970_19625
Reference
1  [PMID:22267672]
  Authors
Qiu J, Ma Y, Wen Y, Chen L, Wu L, Liu W
  Title
Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine.
  Journal
Appl Environ Microbiol 78:2154-60 (2012)
DOI:10.1128/AEM.07025-11
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.4.3.24
IUBMB Enzyme Nomenclature: 1.4.3.24
ExPASy - ENZYME nomenclature database: 1.4.3.24
BRENDA, the Enzyme Database: 1.4.3.24

KEGG   REACTION: R10103
Entry
R10103                      Reaction                               

Name
4-(methylamino)-1-(pyridin-3-yl)butan-1-one:oxygen oxidoreductase (methylamine releasing)
Definition
Pseudooxynicotine + H2O + Oxygen <=> 4-Oxo-1-(3-pyridyl)-1-butanone + Methylamine + Hydrogen peroxide
Equation
Reaction class
RC00225  C19567_C20361
RC00807  C00218_C20361
RC02755  C00007_C00027
Enzyme
Pathway
rn00760  Nicotinate and nicotinamide metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00811  Nicotine degradation, pyrrolidine pathway, nicotine => succinate semialdehyde
Orthology
K20158  pseudooxynicotine oxidase [EC:1.4.3.24]
Other DBs
RHEA: 33906

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