KEGG   ORTHOLOGY: K20262Help
Entry
K20262                      KO                                     

Name
phzG
Definition
dihydrophenazinedicarboxylate synthase [EC:1.10.3.16]
Pathway
ko00405  Phenazine biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
ko02024  Quorum sensing
Module
M00835  Pyocyanine biosynthesis, chorismate => pyocyanine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00405 Phenazine biosynthesis
    K20262  phzG; dihydrophenazinedicarboxylate synthase
 09140 Cellular Processes
  09145 Cellular community - prokaryotes
   02024 Quorum sensing
    K20262  phzG; dihydrophenazinedicarboxylate synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.10  Acting on diphenols and related substances as donors
   1.10.3  With oxygen as acceptor
    1.10.3.16  dihydrophenazinedicarboxylate synthase
     K20262  phzG; dihydrophenazinedicarboxylate synthase
BRITE hierarchy
Other DBs
RN: R11489 R11490 R11491 R11492
COG: COG0259
Genes
SFW: WN53_03370
ECA: ECA2698(ehpE)
PATR: EV46_13180
PATO: GZ59_18620(ehpE)
LAB: LA76x_3331(phzG)
LAQ: GLA29479_2364(phzG)
PAE: PA1905(phzG2) PA4216(phzG1)
PAEV: N297_1965(pdxH) N297_4348(pdxH)
PAEI: N296_1965(pdxH) N296_4348(pdxH)
PAU: PA14_09410(phzG1) PA14_39880(phzG2)
PAG: PLES_07111(phzG1) PLES_34191(phzG2)
PAF: PAM18_0721(phzG1) PAM18_3137(phzG2)
PNC: NCGM2_2822(phzG1) NCGM2_5461(phzG1)
PAEB: NCGM1900_0743(phzG1) NCGM1900_4584(phzG1)
PAEU: BN889_04687(phzG2_1) BN889_06679(phzG2_2)
PAEC: M802_1963(pdxH) M802_4346(pdxH)
PAEO: M801_1964(pdxH) M801_4214(pdxH)
PFB: VO64_0340
BGU: KS03_4989(phzG)
SALS: SLNWT_0234
STRD: NI25_24415
SALC: C2138_10050(pdxH)
SALD: FVA74_10700(pdxH)
BRI: FDF13_09775(pdxH)
NDA: Ndas_5033
APRE: CNX65_16905(pdxH)
ACTA: C1701_23220(pdxH)
ALO: CRK58743
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
  Title
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
  Journal
J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001
  Sequence
[pae:PA4216]
Reference
  Authors
Xu N, Ahuja EG, Janning P, Mavrodi DV, Thomashow LS, Blankenfeldt W
  Title
Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis.
  Journal
Acta Crystallogr D Biol Crystallogr 69:1403-13 (2013)
DOI:10.1107/S0907444913008354

KEGG   ENZYME: 1.10.3.16Help
Entry
EC 1.10.3.16                Enzyme                                 

Name
dihydrophenazinedicarboxylate synthase;
phzG (gene name)
Class
Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
1,4,5a,6,9,10a-hexahydrophenazine-1,6-dicarboxylate:oxygen oxidoreductase
Reaction(IUBMB)
(1) (1R,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylate + O2 = (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate + H2O2 [RN:R11489];
(2) (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate + O2 = (5aS)-5,5a-dihydrophenazine-1,6-dicarboxylate + H2O2 [RN:R11490];
(3) (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylate + O2 = (10aS)-10,10a-dihydrophenazine-1-carboxylate + H2O2 [RN:R11491];
(4) (1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate + O2 = (10aS)-5,10-dihydrophenazine-1-carboxylate + H2O2
Reaction(KEGG)
Substrate
(1R,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylate [CPD:C21408];
O2 [CPD:C00007];
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate [CPD:C21409];
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylate [CPD:C21411];
(1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate [CPD:C21413]
Product
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate [CPD:C21409];
H2O2 [CPD:C00027];
(5aS)-5,5a-dihydrophenazine-1,6-dicarboxylate [CPD:C21410];
(10aS)-10,10a-dihydrophenazine-1-carboxylate [CPD:C21412];
(10aS)-5,10-dihydrophenazine-1-carboxylate
Comment
Requires FMN. The enzyme, isolated from the bacteria Pseudomonas fluorescens 2-79 and Burkholderia lata 383, is involved in biosynthesis of the reduced forms of phenazine, phenazine-1-carboxylate, and phenazine-1,6-dicarboxylate, where it catalyses multiple reactions.
History
EC 1.10.3.16 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K20262  dihydrophenazinedicarboxylate synthase
Genes
SFW: WN53_03370
ECA: ECA2698(ehpE)
PATR: EV46_13180
PATO: GZ59_18620(ehpE)
LAB: LA76x_3331(phzG)
LAQ: GLA29479_2364(phzG)
PAE: PA1905(phzG2) PA4216(phzG1)
PAEV: N297_1965(pdxH) N297_4348(pdxH)
PAEI: N296_1965(pdxH) N296_4348(pdxH)
PAU: PA14_09410(phzG1) PA14_39880(phzG2)
PAG: PLES_07111(phzG1) PLES_34191(phzG2)
PAF: PAM18_0721(phzG1) PAM18_3137(phzG2)
PNC: NCGM2_2822(phzG1) NCGM2_5461(phzG1)
PAEB: NCGM1900_0743(phzG1) NCGM1900_4584(phzG1)
PAEU: BN889_04687(phzG2_1) BN889_06679(phzG2_2)
PAEC: M802_1963(pdxH) M802_4346(pdxH)
PAEO: M801_1964(pdxH) M801_4214(pdxH)
PFB: VO64_0340
BGU: KS03_4989(phzG)
SALS: SLNWT_0234
STRD: NI25_24415
SALC: C2138_10050(pdxH)
SALD: FVA74_10700(pdxH)
BRI: FDF13_09775(pdxH)
NDA: Ndas_5033
APRE: CNX65_16905(pdxH)
ACTA: C1701_23220(pdxH)
ALO: CRK58743
 » show all
Taxonomy
Reference
1  [PMID:23897464]
  Authors
Xu N, Ahuja EG, Janning P, Mavrodi DV, Thomashow LS, Blankenfeldt W
  Title
Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis.
  Journal
Acta Crystallogr D Biol Crystallogr 69:1403-13 (2013)
DOI:10.1107/S0907444913008354
Other DBs
ExplorEnz - The Enzyme Database: 1.10.3.16
IUBMB Enzyme Nomenclature: 1.10.3.16
ExPASy - ENZYME nomenclature database: 1.10.3.16
BRENDA, the Enzyme Database: 1.10.3.16

KEGG   REACTION: R11492Help
Entry
R11492                      Reaction                               

Name
(1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate:oxygen oxidoreductase
Definition
(1R)-1,4,5,10-Tetrahydrophenazine-1-carboxylate + Oxygen <=> 5,10-Dihydrophenazine-1-carboxylate + Hydrogen peroxide
Equation
Reaction class
RC03450  C20981_C21413
Enzyme
Pathway
rn00405  Phenazine biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K20262  dihydrophenazinedicarboxylate synthase [EC:1.10.3.16]
Other DBs
RHEA: 49963

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