KEGG   ORTHOLOGY: K20436Help
Entry
K20436                      KO                                     

Name
valL, salC
Definition
validamine 7-phosphate valienyltransferase [EC:2.5.1.135]
Pathway
ko00525  Acarbose and validamycin biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00815  Validamycin A biosynthesis, sedoheptulopyranose-7P => validamycin A
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00525 Acarbose and validamycin biosynthesis
    K20436  valL, salC; validamine 7-phosphate valienyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.135  validamine 7-phosphate valienyltransferase
     K20436  valL, salC; validamine 7-phosphate valienyltransferase
BRITE hierarchy
Other DBs
RN: R11239 R11250 R11497
COG: COG0380
Genes
SUR: STAUR_3137
SHY: SHJG_0274
SHO: SHJGH_0108
SALS: SLNWT_0581
AMI: Amir_1997
TaxonomyKoalaUniProt
Reference
  Authors
Zheng L, Zhou X, Zhang H, Ji X, Li L, Huang L, Bai L, Zhang H
  Title
Structural and functional analysis of validoxylamine A 7'-phosphate synthase ValL involved in validamycin A biosynthesis.
  Journal
PLoS One 7:e32033 (2012)
DOI:10.1371/journal.pone.0032033
Reference
  Authors
Bai L, Li L, Xu H, Minagawa K, Yu Y, Zhang Y, Zhou X, Floss HG, Mahmud T, Deng Z
  Title
Functional analysis of the validamycin biosynthetic gene cluster and engineered production of validoxylamine A.
  Journal
Chem Biol 13:387-97 (2006)
DOI:10.1016/j.chembiol.2006.02.002
  Sequence

KEGG   ENZYME: 2.5.1.135Help
Entry
EC 2.5.1.135                Enzyme                                 

Name
validamine 7-phosphate valienyltransferase;
vldE (gene name);
valL (gene name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
GDP-valienol:validamine 7-phosphate valienyltransferase
Reaction(IUBMB)
GDP-valienol + validamine 7-phosphate = validoxylamine A 7'-phosphate + GDP [RN:R11497]
Reaction(KEGG)
R11497 > R11239;
(other) R11250
Show
Substrate
GDP-valienol [CPD:C21368];
validamine 7-phosphate [CPD:C21216]
Product
validoxylamine A 7'-phosphate [CPD:C21219];
GDP [CPD:C00035]
Comment
The enzyme, characterized from several Streptomyces strains, is involved in the biosynthesis of the antifungal agent validamycin A.
History
EC 2.5.1.135 created 2016
Pathway
ec00525  Acarbose and validamycin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K20436  validamine 7-phosphate valienyltransferase
Genes
SUR: STAUR_3137
SHY: SHJG_0274
SHO: SHJGH_0108
SALS: SLNWT_0581
AMI: Amir_1997
Taxonomy
Reference
1  [PMID:21766819]
  Authors
Asamizu S, Yang J, Almabruk KH, Mahmud T.
  Title
Pseudoglycosyltransferase catalyzes nonglycosidic C-N coupling in validamycin a biosynthesis.
  Journal
J Am Chem Soc 133:12124-35 (2011)
DOI:10.1021/ja203574u
Reference
2  [PMID:22384130]
  Authors
Zheng L, Zhou X, Zhang H, Ji X, Li L, Huang L, Bai L, Zhang H.
  Title
Structural and functional analysis of validoxylamine A 7'-phosphate synthase ValL involved in validamycin A biosynthesis.
  Journal
PLoS One 7:e32033 (2012)
DOI:10.1371/journal.pone.0032033
  Sequence
[shy:SHJG_0274]
Reference
3  [PMID:23028689]
  Authors
Cavalier MC, Yim YS, Asamizu S, Neau D, Almabruk KH, Mahmud T, Lee YH
  Title
Mechanistic insights into validoxylamine A 7'-phosphate synthesis by VldE using the structure of the entire product complex.
  Journal
PLoS One 7:e44934 (2012)
DOI:10.1371/journal.pone.0044934
  Sequence
[shy:SHJG_0274]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.135
IUBMB Enzyme Nomenclature: 2.5.1.135
ExPASy - ENZYME nomenclature database: 2.5.1.135
BRENDA, the Enzyme Database: 2.5.1.135

KEGG   REACTION: R11250Help
Entry
R11250                      Reaction                               

Definition
NDP-1-epi-valienol + 2-Amino-1,5-anhydro-2-deoxyglucitol <=> Salbostatin 6'-phosphate + NDP
Equation
Reaction class
RC03401  C21210_C21211
Enzyme
Pathway
rn00525  Acarbose and validamycin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K20436  validamine 7-phosphate valienyltransferase [EC:2.5.1.135]

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