KEGG   ORTHOLOGY: K20452Help
Entry
K20452                      KO                                     

Name
dmdA
Definition
dimethylmaleate hydratase large subunit [EC:4.2.1.85]
Pathway
ko00760  Nicotinate and nicotinamide metabolism
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00760 Nicotinate and nicotinamide metabolism
    K20452  dmdA; dimethylmaleate hydratase large subunit
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.85  dimethylmaleate hydratase
     K20452  dmdA; dimethylmaleate hydratase large subunit
BRITE hierarchy
Other DBs
RN: R03069
COG: COG0065
GO: 0047868
Genes
ELM: ELI_2737
AG: ABC88408(dmdA)
TaxonomyKoalaUniProt
Reference
  Authors
Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ.
  Title
Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri.
  Journal
Proc Natl Acad Sci U S A 103:12341-6 (2006)
DOI:10.1073/pnas.0601635103
  Sequence

KEGG   ORTHOLOGY: K20453Help
Entry
K20453                      KO                                     

Name
dmdB
Definition
dimethylmaleate hydratase small subunit [EC:4.2.1.85]
Pathway
ko00760  Nicotinate and nicotinamide metabolism
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00760 Nicotinate and nicotinamide metabolism
    K20453  dmdB; dimethylmaleate hydratase small subunit
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.85  dimethylmaleate hydratase
     K20453  dmdB; dimethylmaleate hydratase small subunit
BRITE hierarchy
Other DBs
RN: R03069
COG: COG0066
GO: 0047868
Genes
ELM: ELI_2736
AG: ABC88409(dmdB)
TaxonomyKoalaUniProt
Reference
  Authors
Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ.
  Title
Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri.
  Journal
Proc Natl Acad Sci U S A 103:12341-6 (2006)
DOI:10.1073/pnas.0601635103
  Sequence

KEGG   ENZYME: 4.2.1.85Help
Entry
EC 4.2.1.85                 Enzyme                                 

Name
dimethylmaleate hydratase;
(2R,3S)-2,3-dimethylmalate hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(2R,3S)-2,3-dimethylmalate hydro-lyase (dimethylmaleate-forming)
Reaction(IUBMB)
(2R,3S)-2,3-dimethylmalate = dimethylmaleate + H2O [RN:R03069]
Reaction(KEGG)
Substrate
(2R,3S)-2,3-dimethylmalate [CPD:C03652]
Product
dimethylmaleate;
H2O [CPD:C00001]
Comment
Requires Fe2+. Inhibited by oxygen.
History
EC 4.2.1.85 created 1989
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K20452  dimethylmaleate hydratase large subunit
K20453  dimethylmaleate hydratase small subunit
Genes
ELM: ELI_2736 ELI_2737
Taxonomy
Reference
1  [PMID:6489933]
  Authors
Kollmann-Koch A, Eggerer H.
  Title
Nicotinic acid metabolism. Dimethylmaleate hydratase.
  Journal
Hoppe Seylers Z Physiol Chem 365:847-57 (1984)
DOI:10.1515/bchm2.1984.365.2.847
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.85
IUBMB Enzyme Nomenclature: 4.2.1.85
ExPASy - ENZYME nomenclature database: 4.2.1.85
BRENDA, the Enzyme Database: 4.2.1.85
CAS: 93229-56-2

KEGG   REACTION: R03069Help
Entry
R03069                      Reaction                               

Name
(2R,3S)-2,3-dimethylmalate hydro-lyase (dimethylmaleate-forming)
Definition
2,3-Dimethylmaleate + H2O <=> (2R,3S)-2,3-Dimethylmalate
Equation
Reaction class
RC00843  C00922_C03652
Enzyme
Pathway
rn00760  Nicotinate and nicotinamide metabolism
rn01120  Microbial metabolism in diverse environments
Orthology
K20452  dimethylmaleate hydratase large subunit [EC:4.2.1.85]
K20453  dimethylmaleate hydratase small subunit [EC:4.2.1.85]
Other DBs
RHEA: 20256

DBGET integrated database retrieval system