KEGG   ORTHOLOGY: K20502
Entry
K20502            Tight     KO                                     

Name
CBDAS
Definition
cannabidiolic acid synthase [EC:1.21.3.8]
Pathway
ko00999  Biosynthesis of various secondary metabolites - part 1
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00894  Cannabidiol biosynthesis, malonyl-CoA => cannabidiol/dronabinol
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various secondary metabolites - part 1
    K20502  CBDAS; cannabidiolic acid synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.21  Catalysing the reaction X-H + Y-H = X-Y
   1.21.3  With oxygen as acceptor
    1.21.3.8  cannabidiolic acid synthase
     K20502  CBDAS; cannabidiolic acid synthase
Other DBs
RN: R10160
Genes
AG: BAF65033(CBDAS)
Reference
  Authors
Taura F, Sirikantaramas S, Shoyama Y, Yoshikai K, Shoyama Y, Morimoto S
  Title
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.
  Journal
FEBS Lett 581:2929-34 (2007)
DOI:10.1016/j.febslet.2007.05.043
  Sequence

KEGG   ENZYME: 1.21.3.8
Entry
EC 1.21.3.8                 Enzyme                                 

Name
cannabidiolic acid synthase;
CBDA synthase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor
Sysname
cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Reaction(IUBMB)
cannabigerolate + O2 = cannabidiolate + H2O2 [RN:R10160]
Reaction(KEGG)
R10160
Substrate
cannabigerolate [CPD:C20406];
O2 [CPD:C00007]
Product
cannabidiolate [CPD:C10784];
H2O2 [CPD:C00027]
Comment
Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.
History
EC 1.21.3.8 created 2012
Pathway
ec00999  Biosynthesis of various secondary metabolites - part 1
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20502  cannabidiolic acid synthase
Reference
1  [PMID:8663284]
  Authors
Taura F, Morimoto S, Shoyama Y
  Title
Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid.
  Journal
J Biol Chem 271:17411-6 (1996)
DOI:10.1074/jbc.271.29.17411
  Sequence
Reference
2  [PMID:17544411]
  Authors
Taura F, Sirikantaramas S, Shoyama Y, Yoshikai K, Shoyama Y, Morimoto S
  Title
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.
  Journal
FEBS Lett 581:2929-34 (2007)
DOI:10.1016/j.febslet.2007.05.043
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.21.3.8
IUBMB Enzyme Nomenclature: 1.21.3.8
ExPASy - ENZYME nomenclature database: 1.21.3.8
BRENDA, the Enzyme Database: 1.21.3.8

KEGG   REACTION: R10160
Entry
R10160                      Reaction                               

Name
cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Definition
Cannabigerolate + Oxygen <=> Cannabidiolic acid + Hydrogen peroxide
Equation
Reaction class
RC02755  C00007_C00027
RC03067  C10784_C20406
Enzyme
Pathway
rn00999  Biosynthesis of various secondary metabolites - part 1
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00894  Cannabidiol biosynthesis, malonyl-CoA => cannabidiol/dronabinol
Orthology
K20502  cannabidiolic acid synthase [EC:1.21.3.8]
Other DBs
RHEA: 34414

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