KEGG   ORTHOLOGY: K20573Help
Entry
K20573                      KO                                     

Name
kanE, kanM2
Definition
2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase [EC:2.4.1.301]
Pathway
ko00524  Neomycin, kanamycin and gentamicin biosynthesis
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00524 Neomycin, kanamycin and gentamicin biosynthesis
    K20573  kanE, kanM2; 2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.301  2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase
     K20573  kanE, kanM2; 2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase
BRITE hierarchy
Other DBs
RN: R10424 R10425 R10426 R10427 R11271 R11272
COG: COG0438
Genes
AG: CAE46941(kanE)
TaxonomyKoalaUniProt
Reference
  Authors
Kharel MK, Subba B, Basnet DB, Woo JS, Lee HC, Liou K, Sohng JK
  Title
A gene cluster for biosynthesis of kanamycin from Streptomyces kanamyceticus: comparison with gentamicin biosynthetic gene cluster.
  Journal
Arch Biochem Biophys 429:204-14 (2004)
DOI:10.1016/j.abb.2004.06.009
  Sequence
Reference
  Authors
Kudo F, Sucipto H, Eguchi T
  Title
Enzymatic activity of a glycosyltransferase KanM2 encoded in the kanamycin biosynthetic gene cluster.
  Journal
J Antibiot (Tokyo) 62:707-10 (2009)
DOI:10.1038/ja.2009.107
Reference
  Authors
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ
  Title
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
  Journal
Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671

KEGG   ENZYME: 2.4.1.301Help
Entry
EC 2.4.1.301                Enzyme                                 

Name
2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase;
kanE (gene name);
kanM2 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-kanosamine:2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase
Reaction(IUBMB)
(1) UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine = UDP + kanamycin A [RN:R10424];
(2) UDP-alpha-D-kanosamine + neamine = UDP + kanamycin B [RN:R10425];
(3) UDP-alpha-D-kanosamine + paromamine = UDP + kanamycin C [RN:R10426];
(4) UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine = UDP + kanamycin X [RN:R10427]
Reaction(KEGG)
Substrate
UDP-alpha-D-kanosamine [CPD:C12211];
2'-deamino-2'-hydroxyneamine [CPD:C20350];
neamine [CPD:C01441];
paromamine [CPD:C01743];
2'-deamino-2'-hydroxyparomamine [CPD:C20353]
Product
UDP [CPD:C00015];
kanamycin A [CPD:C01822];
kanamycin B [CPD:C00825];
kanamycin C [CPD:C01823];
kanamycin X [CPD:C20614]
Comment
Involved in the biosynthetic pathway of kanamycins. The enzyme characterized from the bacterium Streptomyces kanamyceticus can also accept UDP-alpha-D-glucose with lower efficiency [2].
History
EC 2.4.1.301 created 2013
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K20573  2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase
Reference
1  [PMID:19911031]
  Authors
Kudo F, Sucipto H, Eguchi T
  Title
Enzymatic activity of a glycosyltransferase KanM2 encoded in the kanamycin biosynthetic gene cluster.
  Journal
J Antibiot (Tokyo) 62:707-10 (2009)
DOI:10.1038/ja.2009.107
Reference
2  [PMID:21983602]
  Authors
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ
  Title
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
  Journal
Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.301
IUBMB Enzyme Nomenclature: 2.4.1.301
ExPASy - ENZYME nomenclature database: 2.4.1.301
BRENDA, the Enzyme Database: 2.4.1.301

KEGG   REACTION: R11272Help
Entry
R11272                      Reaction                               

Name
UDP-alpha-D-glucose:neamine alpha-D-glucosyltransferase
Definition
UDP-glucose + Neamine <=> UDP + 3''-Deamino-3''-hydroxykanamycin B
Equation
Reaction class
RC00005  C00015_C00029
RC00049  C01441_C21254
Enzyme
Pathway
rn00524  Neomycin, kanamycin and gentamicin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K20573  2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase [EC:2.4.1.301]

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