KEGG   ORTHOLOGY: K20940Help
Entry
K20940                      KO                                     

Name
phzS
Definition
5-methylphenazine-1-carboxylate 1-monooxygenase [EC:1.14.13.218]
Pathway
ko00405  Phenazine biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00835  Pyocyanine biosynthesis, chorismate => pyocyanine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00405 Phenazine biosynthesis
    K20940  phzS; 5-methylphenazine-1-carboxylate 1-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.218  5-methylphenazine-1-carboxylate 1-monooxygenase
     K20940  phzS; 5-methylphenazine-1-carboxylate 1-monooxygenase
BRITE hierarchy
Other DBs
RN: R11346 R11578
COG: COG0654
Genes
ROR: RORB6_06595
RPLN: B1209_13685
RAO: DSD31_13210
EBF: D782_1281
PAO: Pat9b_1855
LAB: LA76x_3342(phzS)
LAQ: GLA29479_2375(phzS)
PAE: PA4217(phzS)
PAEV: N297_4349
PAEI: N296_4349
PAU: PA14_09400(phzS)
PAG: PLES_07101(phzS)
PAF: PAM18_0720(phzS)
PNC: NCGM2_5462(phzS)
PAEB: NCGM1900_0742(phzS)
PAEP: PA1S_03735
PAEM: U769_03765
PAEL: T223_03665
PAEU: BN889_04688(mhbM)
PAEG: AI22_00175
PAEC: M802_4347
PAEO: M801_4215
GAI: IMCC3135_15925(xlnD)
RPI: Rpic_0514
REH: H16_A0578(h16_A0578)
CGD: CR3_2101(ubiH)
BMA: BMAA0391
BMAL: DM55_3356
BMAE: DM78_4291
BMAQ: DM76_4054
BMAI: DM57_4923
BMAF: DM51_4113
BMAZ: BM44_3316
BMAB: BM45_4154
BPS: BPSS2111
BPSE: BDL_5573
BPSM: BBQ_4004(phzS)
BPSU: BBN_5593(phzS)
BPSD: BBX_4740(phzS)
BPK: BBK_4877(phzS)
BPSH: DR55_5621
BPSA: BBU_3886(phzS)
BPSO: X996_5401
BUT: X994_4948
BCEN: DM39_5111
PPUL: RO07_14100
PSPU: NA29_23010
PAPI: SG18_13925
BPT: Bpet0515
PUT: PT7_0833
POL: Bpro_2199
AAV: Aave_0794
AAA: Acav_0721
ACRA: BSY15_1202
DAC: Daci_4386
RTA: Rta_23930
LIM: L103DPR2_01284(xlnD_2)
HYB: Q5W_04140
MES: Meso_2927
OAN: Oant_3996
OAH: DR92_4259
BJA: bll2502
BRA: BRADO0592
BBT: BBta_2684
RPA: RPA3588
RPB: RPB_1945
RPT: Rpal_4108
VGO: GJW-30_1_00854(xlnD_1)
MET: M446_3637
MNO: Mnod_1206
MAQU: Maq22A_c15030(ubiH)
RBM: TEF_16205
SIL: SPO0105
PGD: Gal_00046
LAQU: R2C4_19230
SPSE: SULPSESMR1_00290(hpxO)
RID: RIdsm_02011(xlnD)
NAR: Saro_2238
SWI: Swit_3061
SPHD: HY78_10270
ACR: Acry_0321
TMO: TMO_3126(mhbM)
BKW: BkAM31D_08555(xlnD_1)
MHAS: MHAS_01663(nicC)
MAB: MAB_2237c
MABB: MASS_2167
MCHE: BB28_11255
MSAL: DSM43276_01950(xlnD_2)
SBH: SBI_01059
SVE: SVEN_5103
STRD: NI25_24410
SMAL: SMALA_0139
SFK: KY5_2781c
LMOI: VV02_01275
IDO: I598_2461(hpxO_3)
CFL: Cfla_0298
NDA: Ndas_5023
SRO: Sros_6172
PDX: Psed_3194
PAUT: Pdca_23520
CAI: Caci_4355
SLI: Slin_0336
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
  Title
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
  Journal
J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001
  Sequence
[pae:PA4217]

KEGG   ENZYME: 1.14.13.218Help
Entry
EC 1.14.13.218              Enzyme                                 

Name
5-methylphenazine-1-carboxylate 1-monooxygenase;
phzS (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
5-methylphenazine-1-carboxylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
Reaction(IUBMB)
5-methylphenazine-1-carboxylate + NADH + O2 = pyocyanin + NAD+ + CO2 + H2O [RN:R11346]
Reaction(KEGG)
R11346;
(other) R11578
Show
Substrate
5-methylphenazine-1-carboxylate [CPD:C21295];
NADH [CPD:C00004];
O2 [CPD:C00007]
Product
pyocyanin [CPD:C01748];
NAD+ [CPD:C00003];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. It can also act on phenazine-1-carboxylate, converting it into phenazin-1-ol.
History
EC 1.14.13.218 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K20940  5-methylphenazine-1-carboxylate 1-monooxygenase
Genes
ROR: RORB6_06595
RPLN: B1209_13685
RAO: DSD31_13210
EBF: D782_1281
PAO: Pat9b_1855
LAB: LA76x_3342(phzS)
LAQ: GLA29479_2375(phzS)
PAE: PA4217(phzS)
PAEV: N297_4349
PAEI: N296_4349
PAU: PA14_09400(phzS)
PAG: PLES_07101(phzS)
PAF: PAM18_0720(phzS)
PNC: NCGM2_5462(phzS)
PAEB: NCGM1900_0742(phzS)
PAEP: PA1S_03735
PAEM: U769_03765
PAEL: T223_03665
PAEU: BN889_04688(mhbM)
PAEG: AI22_00175
PAEC: M802_4347
PAEO: M801_4215
GAI: IMCC3135_15925(xlnD)
RPI: Rpic_0514
REH: H16_A0578(h16_A0578)
CGD: CR3_2101(ubiH)
BMA: BMAA0391
BMAL: DM55_3356
BMAE: DM78_4291
BMAQ: DM76_4054
BMAI: DM57_4923
BMAF: DM51_4113
BMAZ: BM44_3316
BMAB: BM45_4154
BPS: BPSS2111
BPSE: BDL_5573
BPSM: BBQ_4004(phzS)
BPSU: BBN_5593(phzS)
BPSD: BBX_4740(phzS)
BPK: BBK_4877(phzS)
BPSH: DR55_5621
BPSA: BBU_3886(phzS)
BPSO: X996_5401
BUT: X994_4948
BCEN: DM39_5111
PPUL: RO07_14100
PSPU: NA29_23010
PAPI: SG18_13925
BPT: Bpet0515
PUT: PT7_0833
POL: Bpro_2199
AAV: Aave_0794
AAA: Acav_0721
ACRA: BSY15_1202
DAC: Daci_4386
RTA: Rta_23930
LIM: L103DPR2_01284(xlnD_2)
HYB: Q5W_04140
MES: Meso_2927
OAN: Oant_3996
OAH: DR92_4259
BJA: bll2502
BRA: BRADO0592
BBT: BBta_2684
RPA: RPA3588
RPB: RPB_1945
RPT: Rpal_4108
VGO: GJW-30_1_00854(xlnD_1)
MET: M446_3637
MNO: Mnod_1206
MAQU: Maq22A_c15030(ubiH)
RBM: TEF_16205
SIL: SPO0105
PGD: Gal_00046
LAQU: R2C4_19230
SPSE: SULPSESMR1_00290(hpxO)
RID: RIdsm_02011(xlnD)
NAR: Saro_2238
SWI: Swit_3061
SPHD: HY78_10270
ACR: Acry_0321
TMO: TMO_3126(mhbM)
BKW: BkAM31D_08555(xlnD_1)
MHAS: MHAS_01663(nicC)
MAB: MAB_2237c
MABB: MASS_2167
MCHE: BB28_11255
MSAL: DSM43276_01950(xlnD_2)
SBH: SBI_01059
SVE: SVEN_5103
STRD: NI25_24410
SMAL: SMALA_0139
SFK: KY5_2781c
LMOI: VV02_01275
IDO: I598_2461(hpxO_3)
CFL: Cfla_0298
NDA: Ndas_5023
SRO: Sros_6172
PDX: Psed_3194
PAUT: Pdca_23520
CAI: Caci_4355
SLI: Slin_0336
 » show all
Taxonomy
Reference
1  [PMID:11591691]
  Authors
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
  Title
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
  Journal
J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001
  Sequence
[pae:PA4217]
Reference
2  [PMID:17253782]
  Authors
Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE
  Title
Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.
  Journal
Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403
Reference
3  [PMID:18416536]
  Authors
Greenhagen BT, Shi K, Robinson H, Gamage S, Bera AK, Ladner JE, Parsons JF
  Title
Crystal structure of the pyocyanin biosynthetic protein PhzS.
  Journal
Biochemistry 47:5281-9 (2008)
DOI:10.1021/bi702480t
  Sequence
[pae:PA4217]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.218
IUBMB Enzyme Nomenclature: 1.14.13.218
ExPASy - ENZYME nomenclature database: 1.14.13.218
BRENDA, the Enzyme Database: 1.14.13.218

KEGG   REACTION: R11346Help
Entry
R11346                      Reaction                               

Name
5-methylphenazine-1-carboxylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
Definition
5-Methylphenazine-1-carboxylate + NADH + Oxygen <=> Pyocyanine + NAD+ + CO2 + H2O
Equation
Reaction class
RC00389  C01748_C21295
Enzyme
Pathway
rn00405  Phenazine biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00835  Pyocyanine biosynthesis, chorismate => pyocyanine
Orthology
K20940  5-methylphenazine-1-carboxylate 1-monooxygenase [EC:1.14.13.218]
Other DBs
RHEA: 48979

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