KEGG   ORTHOLOGY: K20985
Entry
K20985            Tight     KO                                     

Name
LOVG
Definition
dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase [EC:3.1.2.31]
Pathway
ko00999  Biosynthesis of various secondary metabolites - part 1
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00893  Lovastatin biosynthesis, malonyl-CoA => lovastatin acid
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various secondary metabolites - part 1
    K20985  LOVG; dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.2  Thioester hydrolases
    3.1.2.31  dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
     K20985  LOVG; dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
Other DBs
RN: R11187
Genes
AG: EAU29411(LOVG)
Reference
  Authors
Xu W, Chooi YH, Choi JW, Li S, Vederas JC, Da Silva NA, Tang Y
  Title
LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis.
  Journal
Angew Chem Int Ed Engl 52:6472-5 (2013)
DOI:10.1002/anie.201302406
  Sequence

KEGG   ENZYME: 3.1.2.31
Entry
EC 3.1.2.31                 Enzyme                                 

Name
dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase;
LovG
Class
Hydrolases;
Acting on ester bonds;
Thioester hydrolases
Sysname
dihydromonacolin L-[lovastatin nonaketide synthase] hydrolase
Reaction(IUBMB)
dihydromonacolin L-[lovastatin nonaketide synthase] + H2O = holo-[lovastatin nonaketide synthase] + dihydromonacolin L acid
Reaction(KEGG)
(other) R11187
Substrate
dihydromonacolin L-[lovastatin nonaketide synthase];
H2O [CPD:C00001]
Product
holo-[lovastatin nonaketide synthase];
dihydromonacolin L acid [CPD:C20851]
Comment
Dihydromonacolin L acid is synthesized while bound to an acyl-carrier protein domain of the lovastatin nonaketide synthase (EC 2.3.1.161). Since that enzyme lacks a thioesterase domain, release of the dihydromonacolin L acid moiety from the polyketide synthase requires this dedicated enzyme.
History
EC 3.1.2.31 created 2015
Pathway
ec00999  Biosynthesis of various secondary metabolites - part 1
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20985  dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
Reference
1  [PMID:23653178]
  Authors
Xu W, Chooi YH, Choi JW, Li S, Vederas JC, Da Silva NA, Tang Y
  Title
LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis.
  Journal
Angew Chem Int Ed Engl 52:6472-5 (2013)
DOI:10.1002/anie.201302406
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.1.2.31
IUBMB Enzyme Nomenclature: 3.1.2.31
ExPASy - ENZYME nomenclature database: 3.1.2.31
BRENDA, the Enzyme Database: 3.1.2.31

KEGG   REACTION: R11187
Entry
R11187                      Reaction                               

Name
dihydromonacolin L-[acyl-carrier protein] hydrolase
Definition
Dihydromonacolin L-[acyl-carrier protein] + H2O <=> Acyl-carrier protein + Dihydromonacolin L acid
Equation
Comment
Acy-carrier protein is acp domain of LOVB [KO:K20981].
Reaction class
RC00014  C20851_C21108
RC00039  C00229_C21108
Enzyme
Pathway
rn00999  Biosynthesis of various secondary metabolites - part 1
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00893  Lovastatin biosynthesis, malonyl-CoA => lovastatin acid
Orthology
K20985  dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase [EC:3.1.2.31]
Other DBs
RHEA: 11595

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