KEGG   ORTHOLOGY: K21103Help
Entry
K21103                      KO                                     

Name
phzM
Definition
phenazine-1-carboxylate N-methyltransferase [EC:2.1.1.327]
Pathway
ko00405  Phenazine biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00835  Pyocyanine biosynthesis, chorismate => pyocyanine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00405 Phenazine biosynthesis
    K21103  phzM; phenazine-1-carboxylate N-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.327  phenazine-1-carboxylate N-methyltransferase
     K21103  phzM; phenazine-1-carboxylate N-methyltransferase
BRITE hierarchy
Other DBs
RN: R11533
COG: COG2519
GO: 0102168
Genes
PAE: PA4209(phzM)
PAEV: N297_4341
PAEI: N296_4341
PAU: PA14_09490(phzM)
PAP: PSPA7_0889
PAG: PLES_07181(phzM)
PAF: PAM18_0728(phzM)
PNC: NCGM2_5454(phzM)
PAEB: NCGM1900_0750(phzM)
PDK: PADK2_03625
PAEP: PA1S_03775
PAEM: U769_03805
PAEL: T223_03710
PAEU: BN889_04683(phzM)
PAEG: AI22_00135
PAEC: M802_4339
PAEO: M801_4207
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
  Title
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
  Journal
J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001
  Sequence
[pae:PA4209]
Reference
  Authors
Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE
  Title
Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.
  Journal
Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403

KEGG   ENZYME: 2.1.1.327Help
Entry
EC 2.1.1.327                Enzyme                                 

Name
phenazine-1-carboxylate N-methyltransferase;
phzM (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:phenazine-1-carboxylate 5-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + phenazine-1-carboxylate = S-adenosyl-L-homocysteine + 5-methylphenazine-1-carboxylate [RN:R11533]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
phenazine-1-carboxylate [CPD:C21442]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
5-methylphenazine-1-carboxylate [CPD:C21295]
Comment
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. The enzyme is active in vitro only in the presence of EC 1.14.13.218, 5-methylphenazine-1-carboxylate 1-monooxygenase.
History
EC 2.1.1.327 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K21103  phenazine-1-carboxylate N-methyltransferase
Genes
PAE: PA4209(phzM)
PAEV: N297_4341
PAEI: N296_4341
PAU: PA14_09490(phzM)
PAP: PSPA7_0889
PAG: PLES_07181(phzM)
PAF: PAM18_0728(phzM)
PNC: NCGM2_5454(phzM)
PAEB: NCGM1900_0750(phzM)
PDK: PADK2_03625
PAEP: PA1S_03775
PAEM: U769_03805
PAEL: T223_03710
PAEU: BN889_04683(phzM)
PAEG: AI22_00135
PAEC: M802_4339
PAEO: M801_4207
 » show all
Taxonomy
Reference
1  [PMID:17253782]
  Authors
Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE
  Title
Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.
  Journal
Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403
  Sequence
[pae:PA4209]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.327
IUBMB Enzyme Nomenclature: 2.1.1.327
ExPASy - ENZYME nomenclature database: 2.1.1.327
BRENDA, the Enzyme Database: 2.1.1.327

KEGG   REACTION: R11533Help
Entry
R11533                      Reaction                               

Name
S-adenosyl-L-methionine:phenazine-1-carboxylate 5-methyltransferase
Definition
S-Adenosyl-L-methionine + Phenazine-1-carboxylate + H+ <=> S-Adenosyl-L-homocysteine + 5-Methylphenazine-1-carboxylate
Equation
Reaction class
RC03466  C21295_C21442
Enzyme
Pathway
rn00405  Phenazine biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00835  Pyocyanine biosynthesis, chorismate => pyocyanine
Orthology
K21103  phenazine-1-carboxylate N-methyltransferase [EC:2.1.1.327]
Other DBs
RHEA: 49115

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