KEGG   ORTHOLOGY: K21203
Entry
K21203            Tight     KO                                     

Name
OMT3
Definition
(-)-pluviatolide 4-O-methyltransferase [EC:2.1.1.323]
Pathway
ko00998  Biosynthesis of various secondary metabolites - part 2
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various secondary metabolites - part 2
    K21203  OMT3; (-)-pluviatolide 4-O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.323  (-)-pluviatolide 4-O-methyltransferase
     K21203  OMT3; (-)-pluviatolide 4-O-methyltransferase
Other DBs
RN: R11230
Genes
AG: ALG05119(OMT3)
Reference
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence

KEGG   ENZYME: 2.1.1.323
Entry
EC 2.1.1.323                Enzyme                                 

Name
(-)-pluviatolide 4-O-methyltransferase;
OMT3 (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:(-)-pluviatolide 4-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + (-)-pluviatolide = S-adenosyl-L-homocysteine + (-)-bursehernin [RN:R11230]
Reaction(KEGG)
R11230
Substrate
S-adenosyl-L-methionine [CPD:C00019];
(-)-pluviatolide [CPD:C21191]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
(-)-bursehernin [CPD:C21183]
Comment
The enzyme, characterized from the plant Sinopodophyllum hexandrum, is involved in the biosynthetic pathway of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 2.1.1.323 created 2016
Pathway
ec00998  Biosynthesis of various secondary metabolites - part 2
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21203  (-)-pluviatolide 4-O-methyltransferase
Reference
1  [PMID:26359402]
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.323
IUBMB Enzyme Nomenclature: 2.1.1.323
ExPASy - ENZYME nomenclature database: 2.1.1.323
BRENDA, the Enzyme Database: 2.1.1.323

KEGG   REACTION: R11230
Entry
R11230                      Reaction                               

Name
S-adenosyl-L-methionine:(-)-pluviatolide 4-O-methyltransferase
Definition
S-Adenosyl-L-methionine + Pluviatolide <=> S-Adenosyl-L-homocysteine + Bursehernin
Equation
Reaction class
RC00392  C21183_C21191
Enzyme
Pathway
rn00998  Biosynthesis of various secondary metabolites - part 2
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Orthology
K21203  (-)-pluviatolide 4-O-methyltransferase [EC:2.1.1.323]
Other DBs
RHEA: 49039

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