KEGG   ORTHOLOGY: K21205
Entry
K21205            Tight     KO                                     

Name
CYP71CU1
Definition
bursehernin 5'-monooxygenase [EC:1.14.14.131]
Pathway
ko00998  Biosynthesis of various secondary metabolites - part 2
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various secondary metabolites - part 2
    K21205  CYP71CU1; bursehernin 5'-monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K21205  CYP71CU1; bursehernin 5'-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.131  bursehernin 5'-monooxygenase
     K21205  CYP71CU1; bursehernin 5'-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP71 family
   K21205  CYP71CU1; bursehernin 5'-monooxygenase
Other DBs
RN: R11211
Genes
AG: ALG05134(CYP71CU1)
Reference
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence

KEGG   ENZYME: 1.14.14.131
Entry
EC 1.14.14.131              Enzyme                                 

Name
bursehernin 5'-monooxygenase;
CYP71CU1 (gene name);
bursehernin 5'-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(-)-bursehernin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Reaction(IUBMB)
(-)-bursehernin + [reduced NADPH---hemoprotein reductase] + O2 = (-)-5'-demethylyatein + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11211]
Reaction(KEGG)
R11211
Substrate
(-)-bursehernin [CPD:C21183];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-5'-demethylyatein [CPD:C21184];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Sinopodophyllum hexandrum. The enzyme is involved in the biosynthetic pathway of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 1.14.14.131 created 2016 as EC 1.14.13.213, transferred 2018 to EC 1.14.14.131
Pathway
ec00998  Biosynthesis of various secondary metabolites - part 2
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21205  bursehernin 5'-monooxygenase
Reference
1  [PMID:26359402]
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.131
IUBMB Enzyme Nomenclature: 1.14.14.131
ExPASy - ENZYME nomenclature database: 1.14.14.131
BRENDA, the Enzyme Database: 1.14.14.131

KEGG   REACTION: R11211
Entry
R11211                      Reaction                               

Name
(-)-bursehernin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Definition
Bursehernin + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> (-)-5'-Demethylyatein + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Reaction class
RC00046  C21183_C21184
Enzyme
Pathway
rn00998  Biosynthesis of various secondary metabolites - part 2
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Orthology
K21205  bursehernin 5'-monooxygenase [EC:1.14.14.131]
Other DBs
RHEA: 49031

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