KEGG   ORTHOLOGY: K21207
Entry
K21207            Tight     KO                                     

Name
CYP719A23_24
Definition
(-)-pluviatolide synthase [EC:1.14.19.72]
Pathway
ko00998  Biosynthesis of various secondary metabolites - part 2
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various secondary metabolites - part 2
    K21207  CYP719A23_24; (-)-pluviatolide synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K21207  CYP719A23_24; (-)-pluviatolide synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.72  (-)-pluviatolide synthase
     K21207  CYP719A23_24; (-)-pluviatolide synthase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP719 family
   K21207  CYP719A23_24; (-)-pluviatolide synthase
Other DBs
RN: R11222
Genes
AG: AGC29953(CYP719A23) AGC29954(CYP719A24)
Reference
  Authors
Marques JV, Kim KW, Lee C, Costa MA, May GD, Crow JA, Davin LB, Lewis NG
  Title
Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis.
  Journal
J Biol Chem 288:466-79 (2013)
DOI:10.1074/jbc.M112.400689
  Sequence

KEGG   ENZYME: 1.14.19.72
Entry
EC 1.14.19.72               Enzyme                                 

Name
(-)-pluviatolide synthase;
CYP719A23 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
(-)-matairesinol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Reaction(IUBMB)
(-)-matairesinol + [reduced NADPH---hemoprotein reductase] + O2 = (-)-pluviatolide + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11222]
Reaction(KEGG)
R11222
Substrate
(-)-matairesinol [CPD:C10682];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-pluviatolide [CPD:C21191];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plants Sinopodophyllum hexandrum and Podophyllum peltatum catalyses the formation of a methylenedioxy-bridge. It is involved in the biosynthesis of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 1.14.19.72 created 2016 as EC 1.14.21.11, transferred 2018 to EC 1.14.19.72
Pathway
ec00998  Biosynthesis of various secondary metabolites - part 2
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21207  (-)-pluviatolide synthase
Reference
1  [PMID:23161544]
  Authors
Marques JV, Kim KW, Lee C, Costa MA, May GD, Crow JA, Davin LB, Lewis NG
  Title
Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis.
  Journal
J Biol Chem 288:466-79 (2013)
DOI:10.1074/jbc.M112.400689
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.72
IUBMB Enzyme Nomenclature: 1.14.19.72
ExPASy - ENZYME nomenclature database: 1.14.19.72
BRENDA, the Enzyme Database: 1.14.19.72

KEGG   REACTION: R11222
Entry
R11222                      Reaction                               

Name
(-)-matairesinol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Definition
Matairesinol + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Pluviatolide + [Oxidized NADPH---hemoprotein reductase] + 2 H2O
Equation
Reaction class
RC00925  C10682_C21191
Enzyme
Pathway
rn00998  Biosynthesis of various secondary metabolites - part 2
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Orthology
K21207  (-)-pluviatolide synthase [EC:1.14.19.72]
Other DBs
RHEA: 49087

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