KEGG   ORTHOLOGY: K21301Help
Entry
K21301                      KO                                     

Name
oxyR, ctcR
Definition
5a,11a-dehydrotetracycline reductase [EC:1.3.98.4]
Pathway
ko00253  Tetracycline biosynthesis
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00780  Tetracycline/oxytetracycline biosynthesis, pretetramide => tetracycline/oxytetracycline
M00823  Chlortetracycline biosynthesis, pretetramide => chlortetracycline
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K21301  oxyR, ctcR; 5a,11a-dehydrotetracycline reductase
   00253 Tetracycline biosynthesis
    K21301  oxyR, ctcR; 5a,11a-dehydrotetracycline reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.98  With other, known, physiological acceptors
    1.3.98.4  5a,11a-dehydrotetracycline reductase
     K21301  oxyR, ctcR; 5a,11a-dehydrotetracycline reductase
BRITE hierarchy
Other DBs
RN: R05459 R09192
Genes
KAU: B6264_18540
AG: AAZ78341(oxyR) AEI98656(ctcM)
TaxonomyKoalaUniProt
Reference
  Authors
Zhang W, Ames BD, Tsai SC, Tang Y
  Title
Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase.
  Journal
Appl Environ Microbiol 72:2573-80 (2006)
DOI:10.1128/AEM.72.4.2573-2580.2006
  Sequence
Reference
  Authors
Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y
  Title
Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.
  Journal
J Am Chem Soc 135:7138-41 (2013)
DOI:10.1021/ja403516u

KEGG   ENZYME: 1.3.98.4Help
Entry
EC 1.3.98.4                 Enzyme                                 

Name
5a,11a-dehydrotetracycline reductase;
oxyR (gene name);
12-dehydrotetracycline dehydrogenase;
dehydrooxytetracycline dehydrogenase;
12-dehydrotetracycline reductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With other, known, physiological acceptors
BRITE hierarchy
Sysname
tetracycline:coenzyme F420 dehydrogenase
Reaction(IUBMB)
tetracycline + oxidized coenzyme F420 = 5a,11a-dehydrotetracycline + reduced coenzyme F420 [RN:R05459]
Reaction(KEGG)
R05459;
(other) R09192
Show
Substrate
tetracycline [CPD:C06570];
oxidized coenzyme F420 [CPD:C00876]
Product
5a,11a-dehydrotetracycline [CPD:C03206];
reduced coenzyme F420 [CPD:C01080]
Comment
The enzyme, characterized from the bacteria Streptomyces aureofaciens and Streptomyces rimosus, catalyses the last step in the biosynthesis of the tetracycline antibiotics tetracycline and oxytetracycline.
History
EC 1.3.98.4 created 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K21301  5a,11a-dehydrotetracycline reductase
Genes
KAU: B6264_18540
Taxonomy
Reference
1
  Authors
McCormick, J.R.D., Hirsch, U., Sjolander, N.O. and Doerschuk, A.P.
  Title
Cosynthesis of tetracyclines by pairs of Streptomyces aureofaciens mutants.
  Journal
J Am Chem Soc 82:5006-5007 (1960)
Reference
2
  Authors
Miller, P.A., Sjolander, N.O., Nalesnyk, S., Arnold, N., Johnson, S., Doerschuk, A.P. and McCormick, J.R.D.
  Title
Cosynthetic factor I, a factor involved in hydrogen-transfer in Streptomyces aureofaciens.
  Journal
J Am Chem Soc 82:5002-5003 (1960)
Reference
3
  Authors
McCormick, J.R.D. and Morton, G.O.
  Title
Identity of cosynthetic factor I of Streptomyces aureofaciens and fragment FO from coenzyme F420 of Methanobacterium species.
  Journal
J Am Chem Soc 104:4014-4015 (1982)
Reference
4  [PMID:23621493]
  Authors
Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y
  Title
Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.
  Journal
J Am Chem Soc 135:7138-41 (2013)
DOI:10.1021/ja403516u
Other DBs
ExplorEnz - The Enzyme Database: 1.3.98.4
IUBMB Enzyme Nomenclature: 1.3.98.4
ExPASy - ENZYME nomenclature database: 1.3.98.4
BRENDA, the Enzyme Database: 1.3.98.4

KEGG   REACTION: R09192Help
Entry
R09192                      Reaction                               

Name
oxytetracycline:coenzyme-F420 oxidoreductase
Definition
Oxytetracycline + Coenzyme F420 <=> 5a,11a-Dehydrooxytetracycline + Reduced coenzyme F420
Equation
Reaction class
RC02461  C06624_C18296
Enzyme
Pathway
rn00253  Tetracycline biosynthesis
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00780  Tetracycline/oxytetracycline biosynthesis, pretetramide => tetracycline/oxytetracycline
Orthology
K21301  5a,11a-dehydrotetracycline reductase [EC:1.3.98.4]
Other DBs
RHEA: 50395

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