KEGG   ORTHOLOGY: K21782
Entry
K21782                      KO                                     

Name
pigA, redW
Definition
L-prolyl-PCP dehydrogenase [EC:1.3.8.14]
Pathway
ko00333  Prodigiosin biosynthesis
ko01100  Metabolic pathways
Module
M00837  Prodigiosin biosynthesis, L-proline => prodigiosin
M00838  Undecylprodigiosin biosynthesis, L-proline => undecylprodigiosin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00333 Prodigiosin biosynthesis
    K21782  pigA, redW; L-prolyl-PCP dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.8  With a flavin as acceptor
    1.3.8.14  L-prolyl-[peptidyl-carrier protein] dehydrogenase
     K21782  pigA, redW; L-prolyl-PCP dehydrogenase
Other DBs
RN: R06747
COG: COG1960
Genes
SMW: SMWW4_v1c11040(pigA)
SRR: SerAS9_1753
SRS: SerAS12_1753
SRA: SerAS13_1754
SERF: L085_23030
SERA: Ser39006_020695
SERQ: CWC46_20700
SERM: CLM71_22610
VGA: BSQ33_17325
PRR: AT705_06580
HCH: HCH_06021
SCO: SCO5879(redW)
STRD: NI25_09835
AG: CAH55629(pigA)
 » show all
Reference
  Authors
Williamson NR, Simonsen HT, Ahmed RA, Goldet G, Slater H, Woodley L, Leeper FJ, Salmond GP
  Title
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces.
  Journal
Mol Microbiol 56:971-89 (2005)
DOI:10.1111/j.1365-2958.2005.04602.x
  Sequence
Reference
  Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
  Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
  Journal
Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0
  Sequence

KEGG   ENZYME: 1.3.8.14
Entry
EC 1.3.8.14                 Enzyme                                 

Name
L-prolyl-[peptidyl-carrier protein] dehydrogenase;
pigA (gene name);
bmp3 (gene name);
pltE (gene name);
redW (gene name);
(L-prolyl)-[peptidyl-carrier protein]:electron-transfer flavoprotein oxidoreductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With a flavin as acceptor
Sysname
L-prolyl-[peptidyl-carrier protein]:electron-transfer flavoprotein oxidoreductase
Reaction(IUBMB)
L-prolyl-[peptidyl-carrier protein] + 2 electron-transfer flavoprotein = 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] + 2 reduced electron-transfer flavoprotein [RN:R06747]
Reaction(KEGG)
R06747
Substrate
L-prolyl-[peptidyl-carrier protein] [CPD:C12470];
electron-transfer flavoprotein [CPD:C04253]
Product
1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] [CPD:C12483];
reduced electron-transfer flavoprotein [CPD:C04570]
Comment
Contains FAD. The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It is believed to catalyse the formation of a Delta2-pyrrolin-2-carbonyl-[peptidyl-carrier protein] intermediate, followed by a two-electron oxidation to 1H-pyrrol-2-carbonyl-[peptidyl-carrier protein].
History
EC 1.3.8.14 created 2017
Pathway
ec00333  Prodigiosin biosynthesis
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12721  L-prolyl-[peptidyl-carrier protein] dehydrogenase
K21782  L-prolyl-PCP dehydrogenase
Genes
SMW: SMWW4_v1c11040(pigA)
SRR: SerAS9_1753
SRS: SerAS12_1753
SRA: SerAS13_1754
SERF: L085_23030
SERA: Ser39006_020695
SERQ: CWC46_20700
SERM: CLM71_22610
VGA: BSQ33_17325
PRR: AT705_06580
HCH: HCH_06021
SCO: SCO5879(redW)
STRD: NI25_09835
 » show all
Reference
1  [PMID:11880032]
  Authors
Thomas MG, Burkart MD, Walsh CT.
  Title
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.
  Journal
Chem Biol 9:171-84 (2002)
DOI:10.1016/S1074-5521(02)00100-X
  Sequence
[pfl:PFL_2791] [sco:SCO5879]
Reference
2  [PMID:15528645]
  Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
  Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
  Journal
Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.8.14
IUBMB Enzyme Nomenclature: 1.3.8.14
ExPASy - ENZYME nomenclature database: 1.3.8.14
BRENDA, the Enzyme Database: 1.3.8.14

KEGG   REACTION: R06747
Entry
R06747                      Reaction                               

Name
L-prolyl-[peptidyl-carrier protein]:electron-transfer flavoprotein oxidoreductase
Definition
L-Prolyl-[pcp] + 2 Electron-transferring flavoprotein <=> Pyrrole-2-carbonyl-[pcp] + 2 Reduced electron-transferring flavoprotein
Equation
C12470 + 2 C04253 <=> C12483 + 2 C04570
Reaction class
RC03357  C12470_C12483
Enzyme
Pathway
rn00333  Prodigiosin biosynthesis
rn00401  Novobiocin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00837  Prodigiosin biosynthesis, L-proline => prodigiosin
M00838  Undecylprodigiosin biosynthesis, L-proline => undecylprodigiosin
Orthology
K12721  L-prolyl-[peptidyl-carrier protein] dehydrogenase [EC:1.3.8.14]
K21782  L-prolyl-PCP dehydrogenase [EC:1.3.8.14]
Other DBs
RHEA: 55155

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