KEGG   ORTHOLOGY: K21819
Entry
K21819            Tight     KO                                     
Symbol
EASG
Name
agroclavine dehydrogenase [EC:1.5.1.46]
Pathway
map00901  Indole alkaloid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R10229  agroclavine:NADP+ oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00901 Indole alkaloid biosynthesis
    K21819  EASG; agroclavine dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.46  agroclavine dehydrogenase
     K21819  EASG; agroclavine dehydrogenase
Genes
AG: AAW57089(easG)
Reference
PMID:21494745
  Authors
Matuschek M, Wallwey C, Xie X, Li SM
  Title
New insights into ergot alkaloid biosynthesis in Claviceps purpurea: an agroclavine synthase EasG catalyses, via a non-enzymatic adduct with reduced glutathione, the conversion of chanoclavine-I aldehyde to agroclavine.
  Journal
Org Biomol Chem 9:4328-35 (2011)
DOI:10.1039/c0ob01215g
  Sequence
[ag:AAW57089]

  All links  
No link information was found.
KEGG   ENZYME: 1.5.1.46
Entry
EC 1.5.1.46                 Enzyme                                 
Name
agroclavine dehydrogenase;
easG (gene name)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
agroclavine:NADP+ oxidoreductase
Reaction(IUBMB)
agroclavine + NADP+ = 6,8-dimethyl-6,7,8,9-tetradehydroergoline + NADPH + H+ [RN:R10229]
Reaction(KEGG)
R10229
Substrate
agroclavine [CPD:C09023];
NADP+ [CPD:C00006]
Product
6,8-dimethyl-6,7,8,9-tetradehydroergoline [CPD:C20457];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme participates in the biosynthesis of ergotamine, an ergot alkaloid produced by some fungi of the Clavicipitaceae family. The reaction is catalysed in the opposite direction to that shown. The substrate for the enzyme is an iminium intermediate that is formed spontaneously from chanoclavine-I aldehyde in the presence of glutathione.
History
EC 1.5.1.46 created 2013
Pathway
ec00901  Indole alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K21819  agroclavine dehydrogenase
Reference
1  [PMID:21494745]
  Authors
Matuschek M, Wallwey C, Xie X, Li SM
  Title
New insights into ergot alkaloid biosynthesis in Claviceps purpurea: an agroclavine synthase EasG catalyses, via a non-enzymatic adduct with reduced glutathione, the conversion of chanoclavine-I aldehyde to agroclavine.
  Journal
Org Biomol Chem 9:4328-35 (2011)
DOI:10.1039/c0ob01215g
  Sequence
[ag:AAW57089]
Other DBs
ExplorEnz - The Enzyme Database: 1.5.1.46
IUBMB Enzyme Nomenclature: 1.5.1.46
ExPASy - ENZYME nomenclature database: 1.5.1.46
BRENDA, the Enzyme Database: 1.5.1.46

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Protein sequence (8)   
   UniProt (4)   
   SWISS-PROT (4)   
Protein domain (4)   
   InterPro (4)   
All databases (12)   

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KEGG   REACTION: R10229
Entry
R10229                      Reaction                               
Name
agroclavine:NADP+ oxidoreductase
Definition
Agroclavine + NADP+ <=> 6,8-Dimethyl-6,7,8,9-tetradehydroergoline + NADPH + H+
Equation
C09023 + C00006 <=> C20457 + C00005 + C00080
Reaction class
RC00001  C00005_C00006
RC03091  C09023_C20457
Enzyme
1.5.1.46
Pathway
rn00901  Indole alkaloid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.46
     R10229  Agroclavine + NADP+ <=> 6,8-Dimethyl-6,7,8,9-tetradehydroergoline + NADPH + H+
Orthology
K21819  agroclavine dehydrogenase [EC:1.5.1.46]
Other DBs
RHEA: 34062

  All links  
No link information was found.
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