KEGG   ORTHOLOGY: K22113
Entry
K22113                      KO                                     

Name
ddaG
Definition
fumarate---(S)-2,3-diaminopropanoate ligase [EC:6.3.2.46]
Pathway
ko00997  Biosynthesis of various secondary metabolites - part 3
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00904  Dapdiamides biosynthesis, L-2,3-diaminopropanoate => dapdiamide A/B/C
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various secondary metabolites - part 3
    K22113  ddaG; fumarate---(S)-2,3-diaminopropanoate ligase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.46  fumarate---(S)-2,3-diaminopropanoate ligase
     K22113  ddaG; fumarate---(S)-2,3-diaminopropanoate ligase
Other DBs
RN: R10940
Genes
SPE: Spro_0345
SPLY: Q5A_016900
PVA: Pvag_pPag20164
PAGC: BEE12_22715
AG: ADN39487(ddaG)
Reference
  Authors
Hollenhorst MA, Clardy J, Walsh CT
  Title
The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics.
  Journal
Biochemistry 48:10467-72 (2009)
DOI:10.1021/bi9013165
  Sequence

KEGG   ENZYME: 6.3.2.46
Entry
EC 6.3.2.46                 Enzyme                                 

Name
fumarate---(S)-2,3-diaminopropanoate ligase;
DdaG;
fumarate:(S)-2,3-diaminopropanoate ligase (AMP-forming)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
fumarate:L-2,3-diaminopropanoate ligase (AMP-forming)
Reaction(IUBMB)
ATP + fumarate + L-2,3-diaminopropanoate = AMP + diphosphate + N3-fumaroyl-L-2,3-diaminopropanoate [RN:R10940]
Reaction(KEGG)
R10940
Substrate
ATP [CPD:C00002];
fumarate [CPD:C00122];
L-2,3-diaminopropanoate [CPD:C03401]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
N3-fumaroyl-L-2,3-diaminopropanoate [CPD:C20961]
Comment
The enzyme, characterized from the bacterium Enterobacter agglomerans, is involved in biosynthesis of dapdiamide tripeptide antibiotics, a family of fumaramoyl- and epoxysuccinamoyl-peptides named for the presence of an L-2,3-diaminopropanoate (DAP) moiety and two amide linkages in their scaffold.
History
EC 6.3.2.46 created 2015
Pathway
ec00997  Biosynthesis of various secondary metabolites - part 3
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22113  fumarate---(S)-2,3-diaminopropanoate ligase
Genes
SPE: Spro_0345
SPLY: Q5A_016900
PVA: Pvag_pPag20164
PAGC: BEE12_22715
Reference
1  [PMID:19807062]
  Authors
Hollenhorst MA, Clardy J, Walsh CT
  Title
The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics.
  Journal
Biochemistry 48:10467-72 (2009)
DOI:10.1021/bi9013165
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.46
IUBMB Enzyme Nomenclature: 6.3.2.46
ExPASy - ENZYME nomenclature database: 6.3.2.46
BRENDA, the Enzyme Database: 6.3.2.46

KEGG   REACTION: R10940
Entry
R10940                      Reaction                               

Name
fumarate:L-2,3-diaminopropanoate ligase (AMP-forming)
Definition
ATP + Fumarate + L-2,3-Diaminopropanoate <=> AMP + Diphosphate + N3-Fumaroyl-L-2,3-diaminopropanoate
Equation
Reaction class
RC00096  C03401_C20961
RC00957  C00122_C20961
Enzyme
Pathway
rn00997  Biosynthesis of various secondary metabolites - part 3
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00904  Dapdiamides biosynthesis, L-2,3-diaminopropanoate => dapdiamide A/B/C
Orthology
K22113  fumarate---(S)-2,3-diaminopropanoate ligase [EC:6.3.2.46]
Other DBs
RHEA: 44343

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