KEGG   ORTHOLOGY: K22270Help
Entry
K22270                      KO                                     

Name
nagX
Definition
3-hydroxybenzoate 6-monooxygenase [EC:1.14.13.24]
Pathway
ko00362  Benzoate degradation
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K22270  nagX; 3-hydroxybenzoate 6-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.24  3-hydroxybenzoate 6-monooxygenase
     K22270  nagX; 3-hydroxybenzoate 6-monooxygenase
BRITE hierarchy
Other DBs
RN: R02589
COG: COG0654
GO: 0018669
Genes
ECE: Z3390
ECS: ECs3027
ECF: ECH74115_3267
ETW: ECSP_3013
ELX: CDCO157_2790
ECOO: ECRM13514_5372
ECOH: ECRM13516_3724
EOK: G2583_2678
ELR: ECO55CA74_13240
EAL: EAKF1_ch3857
STY: STY2405
STT: t0680
STM: STM2175
SENI: CY43_11700
SPT: SPA0676
SEK: SSPA0635
SEI: SPC_1527
SEC: SCH_2192(nhg)
SHB: SU5_02767
SENS: Q786_10795
SED: SeD_A2522
SEG: SG2211
SEL: SPUL_0714
SET: SEN2168
SENA: AU38_10960
SENO: AU37_10970
SENV: AU39_10970
SENQ: AU40_12270
SENL: IY59_11265
SEEP: I137_03245
SENE: IA1_10880
SBG: SBG_1987
SBZ: A464_2299
KOX: KOX_25675
KOE: A225_4035
EAE: EAE_23875
EAR: CCG29101
CKO: CKO_00654
CRO: ROD_22631
CAMA: F384_11420
RTG: NCTC13098_02137(mhbM)
EBF: D782_1486
SMAR: SM39_2826(mhbM)
SMAC: SMDB11_2639(mhbM)
XBV: XBW1_2121(mhbM)
PEN: PSEEN2596(mhbM)
PSEM: TO66_18335
OCE: GU3_03005
PSE: NH8B_2047
REH: H16_B0876(h16_B0876)
CNC: CNE_2c08340(mhbM) CNE_BB2p00890(xlnD)
BCN: Bcen_4218
BCJ: BCAM1274(mhbM)
BCEN: DM39_3727(mhbM)
BCEO: I35_5123(mhbM)
BMU: Bmul_4439
BMJ: BMULJ_04070(nahG)
BMK: DM80_3604(mhbM)
BMUL: NP80_4175(mhbM)
BCT: GEM_4469
BCED: DM42_3818(mhbM)
BCON: NL30_06230
BTEI: WS51_04055
BPSL: WS57_07660
BMEC: WJ16_24850
BGO: BM43_4139(nagX)
PPNO: DA70_13155
PPNM: LV28_17545
PPUL: RO07_11650
PSPU: NA29_07575
PAPI: SG18_11980
BAV: BAV1787
BHZ: ACR54_02471(mhbM)
AXY: AXYL_05897(mhbM)
AXX: ERS451415_00540(mhbM)
POL: Bpro_2128
PNA: Pnap_3144
DAC: Daci_3703
RTA: Rta_17950
LIH: L63ED372_01176(nagX)
HYR: BSY239_3884(nagX)
LCH: Lcho_3669
EBA: ebA1378(s5h)
AZO: azo2422(nahG)
AOA: dqs_2566
TCL: Tchl_1075
MES: Meso_0052
OCH: CES85_5237(xlnD)
RBM: TEF_07880
SIL: SPO3692
JAN: Jann_2908
PGD: Gal_04153
CID: P73_1457
BACO: OXB_0838
BEO: BEH_11950
BKW: BkAM31D_03935(mhbM) BkAM31D_23545(xlnD_2)
SIV: SSIL_3714
SAY: TPY_1281
MAB: MAB_0288
MABB: MASS_0290
MCHE: BB28_01425
MSAL: DSM43276_00244(xlnD_1)
CGL: NCgl2923(Cgl3026)
CGB: cg3354
CGU: WA5_2923
CGT: cgR_2913
CGM: cgp_3354
CGJ: AR0_14655
CEF: CE2863
ROP: ROP_15470
GRU: GCWB2_11220(mhbM)
SALB: XNR_0599
SGU: SGLAU_17245(xlnD1) SGLAU_17380(xlnD2)
STRM: M444_18400
SAMB: SAM23877_1103(mhbM)
SRW: TUE45_05046(xlnD)
STRD: NI25_24455
SALJ: SMD11_3540
KSK: KSE_58270
ART: Arth_2055
AAU: AAur_0334
ACH: Achl_0762
CFI: Celf_3007
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Park M, Jeon Y, Jang HH, Ro HS, Park W, Madsen EL, Jeon CO
  Title
Molecular and biochemical characterization of 3-hydroxybenzoate 6-hydroxylase from Polaromonas naphthalenivorans CJ2.
  Journal
Appl Environ Microbiol 73:5146-52 (2007)
DOI:10.1128/AEM.00782-07
  Sequence
[pna:Pnap_3144]
Reference
  Authors
Yang YF, Zhang JJ, Wang SH, Zhou NY
  Title
Purification and characterization of the ncgl2923 -encoded 3-hydroxybenzoate 6-hydroxylase from Corynebacterium glutamicum.
  Journal
J Basic Microbiol 50:599-604 (2010)
DOI:10.1002/jobm.201000053
  Sequence
[cgl:NCgl2923]

KEGG   ENZYME: 1.14.13.24Help
Entry
EC 1.14.13.24               Enzyme                                 

Name
3-hydroxybenzoate 6-monooxygenase;
3-hydroxybenzoate 6-hydroxylase;
m-hydroxybenzoate 6-hydroxylase;
3-hydroxybenzoic acid-6-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R02589]
Reaction(KEGG)
Substrate
3-hydroxybenzoate [CPD:C00587];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions; NADPH can act instead of NADH, but more slowly.
History
EC 1.14.13.24 created 1984
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K22270  3-hydroxybenzoate 6-monooxygenase
Genes
ECE: Z3390
ECS: ECs3027
ECF: ECH74115_3267
ETW: ECSP_3013
ELX: CDCO157_2790
ECOO: ECRM13514_5372
ECOH: ECRM13516_3724
EOK: G2583_2678
ELR: ECO55CA74_13240
EAL: EAKF1_ch3857
STY: STY2405
STT: t0680
STM: STM2175
SENI: CY43_11700
SPT: SPA0676
SEK: SSPA0635
SEI: SPC_1527
SEC: SCH_2192(nhg)
SHB: SU5_02767
SENS: Q786_10795
SED: SeD_A2522
SEG: SG2211
SEL: SPUL_0714
SET: SEN2168
SENA: AU38_10960
SENO: AU37_10970
SENV: AU39_10970
SENQ: AU40_12270
SENL: IY59_11265
SEEP: I137_03245
SENE: IA1_10880
SBG: SBG_1987
SBZ: A464_2299
KOX: KOX_25675
KOE: A225_4035
EAE: EAE_23875
EAR: CCG29101
CKO: CKO_00654
CRO: ROD_22631
CAMA: F384_11420
RTG: NCTC13098_02137(mhbM)
EBF: D782_1486
SMAR: SM39_2826(mhbM)
SMAC: SMDB11_2639(mhbM)
XBV: XBW1_2121(mhbM)
PEN: PSEEN2596(mhbM)
PSEM: TO66_18335
OCE: GU3_03005
PSE: NH8B_2047
REH: H16_B0876(h16_B0876)
CNC: CNE_2c08340(mhbM) CNE_BB2p00890(xlnD)
BCN: Bcen_4218
BCJ: BCAM1274(mhbM)
BCEN: DM39_3727(mhbM)
BCEO: I35_5123(mhbM)
BMU: Bmul_4439
BMJ: BMULJ_04070(nahG)
BMK: DM80_3604(mhbM)
BMUL: NP80_4175(mhbM)
BCT: GEM_4469
BCED: DM42_3818(mhbM)
BCON: NL30_06230
BTEI: WS51_04055
BPSL: WS57_07660
BMEC: WJ16_24850
BGO: BM43_4139(nagX)
PPNO: DA70_13155
PPNM: LV28_17545
PPUL: RO07_11650
PSPU: NA29_07575
PAPI: SG18_11980
BAV: BAV1787
BHZ: ACR54_02471(mhbM)
AXY: AXYL_05897(mhbM)
AXX: ERS451415_00540(mhbM)
POL: Bpro_2128
PNA: Pnap_3144
DAC: Daci_3703
RTA: Rta_17950
LIH: L63ED372_01176(nagX)
HYR: BSY239_3884(nagX)
LCH: Lcho_3669
EBA: ebA1378(s5h)
AZO: azo2422(nahG)
AOA: dqs_2566
TCL: Tchl_1075
MES: Meso_0052
OCH: CES85_5237(xlnD)
RBM: TEF_07880
SIL: SPO3692
JAN: Jann_2908
PGD: Gal_04153
CID: P73_1457
BACO: OXB_0838
BEO: BEH_11950
BKW: BkAM31D_03935(mhbM) BkAM31D_23545(xlnD_2)
SIV: SSIL_3714
SAY: TPY_1281
MAB: MAB_0288
MABB: MASS_0290
MCHE: BB28_01425
MSAL: DSM43276_00244(xlnD_1)
CGL: NCgl2923(Cgl3026)
CGB: cg3354
CGU: WA5_2923
CGT: cgR_2913
CGM: cgp_3354
CGJ: AR0_14655
CEF: CE2863
ROP: ROP_15470
GRU: GCWB2_11220(mhbM)
SALB: XNR_0599
SGU: SGLAU_17245(xlnD1) SGLAU_17380(xlnD2)
STRM: M444_18400
SAMB: SAM23877_1103(mhbM)
SRW: TUE45_05046(xlnD)
STRD: NI25_24455
SALJ: SMD11_3540
KSK: KSE_58270
ART: Arth_2055
AAU: AAur_0334
ACH: Achl_0762
CFI: Celf_3007
 » show all
Taxonomy
Reference
1  [PMID:4357436]
  Authors
Groseclose EE, Ribbons DW, Hughes H.
  Title
3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa.
  Journal
Biochem Biophys Res Commun 55:897-903 (1973)
DOI:10.1016/0006-291X(73)91228-X
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.24
IUBMB Enzyme Nomenclature: 1.14.13.24
ExPASy - ENZYME nomenclature database: 1.14.13.24
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.24
BRENDA, the Enzyme Database: 1.14.13.24
CAS: 51570-26-4

KEGG   REACTION: R02589Help
Entry
R02589                      Reaction                               

Name
3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating)
Definition
3-Hydroxybenzoate + Oxygen + NADH + H+ <=> 2,5-Dihydroxybenzoate + NAD+ + H2O
Equation
Reaction class
RC00001  C00003_C00004
RC00046  C00587_C00628
Enzyme
Pathway
rn00362  Benzoate degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K22270  3-hydroxybenzoate 6-monooxygenase [EC:1.14.13.24]
Other DBs
RHEA: 22695

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