KEGG   ORTHOLOGY: K22436
Entry
K22436            Tight     KO                                     

Name
PSIM
Definition
psilocybin synthase [EC:2.1.1.345]
Pathway
ko00901  Indole alkaloid biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00901 Indole alkaloid biosynthesis
    K22436  PSIM; psilocybin synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.345  psilocybin synthase
     K22436  PSIM; psilocybin synthase
Other DBs
RN: R11934 R11935 R11936
COG: COG3129
Genes
AG: ASU62238(psiM)
Reference
  Authors
Fricke J, Blei F, Hoffmeister D
  Title
Enzymatic Synthesis of Psilocybin.
  Journal
Angew Chem Int Ed Engl 56:12352-12355 (2017)
DOI:10.1002/anie.201705489
  Sequence

KEGG   ENZYME: 2.1.1.345
Entry
EC 2.1.1.345                Enzyme                                 

Name
psilocybin synthase;
PsiM
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:4-hydroxytryptamine-4-phosphate N,N-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate = 2 S-adenosyl-L-homocysteine + psilocybin (overall reaction) [RN:R11934];
(1a) S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate = S-adenosyl-L-homocysteine + 4-hydroxy-N-methyltryptamine 4-phosphate [RN:R11935];
(1b) S-adenosyl-L-methionine + 4-hydroxy-N-methyltryptamine 4-phosphate = S-adenosyl-L-homocysteine + psilocybin [RN:R11936]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
4-hydroxytryptamine 4-phosphate [CPD:C21778];
4-hydroxy-N-methyltryptamine 4-phosphate [CPD:C21779]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
psilocybin [CPD:C07576];
4-hydroxy-N-methyltryptamine 4-phosphate [CPD:C21779]
Comment
Isolated from the fungus Psilocybe cubensis. The product, psilocybin, is a psychoactive compound.
History
EC 2.1.1.345 created 2017
Pathway
ec00901  Indole alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22436  psilocybin synthase
Reference
1  [PMID:28763571]
  Authors
Fricke J, Blei F, Hoffmeister D
  Title
Enzymatic Synthesis of Psilocybin.
  Journal
Angew Chem Int Ed Engl 56:12352-12355 (2017)
DOI:10.1002/anie.201705489
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.345
IUBMB Enzyme Nomenclature: 2.1.1.345
ExPASy - ENZYME nomenclature database: 2.1.1.345
BRENDA, the Enzyme Database: 2.1.1.345

KEGG   REACTION: R11935
Entry
R11935                      Reaction                               

Name
S-adenosyl-L-methionine:norbaeocystin N-methyltransferase
Definition
S-Adenosyl-L-methionine + Norbaeocystin <=> S-Adenosyl-L-homocysteine + Baeocystin
Equation
Reaction class
RC00060  C21778_C21779
Enzyme
Pathway
rn00901  Indole alkaloid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K22436  psilocybin synthase [EC:2.1.1.345]
Other DBs
RHEA: 55575

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