KEGG   ORTHOLOGY: K23082
Entry
K23082                      KO                                     

Name
kdoH1
Definition
3-deoxy-D-manno-octulosonic acid-hydrolase [EC:3.2.1.144]
Pathway
ko00540  Lipopolysaccharide biosynthesis
ko01100  Metabolic pathways
Module
M00867  KDO2-lipid A modification pathway
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09107 Glycan biosynthesis and metabolism
   00540 Lipopolysaccharide biosynthesis
    K23082  kdoH1; 3-deoxy-D-manno-octulosonic acid-hydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.2  Glycosylases
   3.2.1  Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
    3.2.1.144  3-deoxyoctulosonase
     K23082  kdoH1; 3-deoxy-D-manno-octulosonic acid-hydrolase
Other DBs
RN: R12219
GO: 0050534
CAZy: GT33
Genes
LPN: lpg2938
LPO: LPO_3263
LPU: LPE509_00072
LPM: LP6_2973
LPE: lp12_2927
UPI: EJG51_009680
HPY: HP0579
HEO: C694_02985
HPJ: jhp_0526
HPA: HPAG1_0558
HPG: HPG27_538
HPL: HPB8_775
HPZ: HPKB_0765
HPD: KHP_0739
HEY: MWE_0938
HPYR: K747_06965
HPYI: K750_05175
HPYU: K751_03515
HPYM: K749_01605
HEB: U063_0884
HEZ: U064_0888
HAC: Hac_1433
HMS: HMU03740
HCE: HCW_06240
HCM: HCD_01360
 » show all
Reference
  Authors
Stead CM, Zhao J, Raetz CR, Trent MS
  Title
Removal of the outer Kdo from Helicobacter pylori lipopolysaccharide and its impact on the bacterial surface.
  Journal
Mol Microbiol 78:837-52 (2010)
DOI:10.1111/j.1365-2958.2010.07304.x
  Sequence
[hpy:HP0579]

KEGG   ORTHOLOGY: K23083
Entry
K23083                      KO                                     

Name
kdoH2
Definition
3-deoxy-D-manno-octulosonic acid-hydrolase
Pathway
ko00540  Lipopolysaccharide biosynthesis
ko01100  Metabolic pathways
Module
M00867  KDO2-lipid A modification pathway
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09107 Glycan biosynthesis and metabolism
   00540 Lipopolysaccharide biosynthesis
    K23083  kdoH2; 3-deoxy-D-manno-octulosonic acid-hydrolase
Other DBs
RN: R12219
COG: COG4692
Genes
LPN: lpg2939
LPO: LPO_3264
LPU: LPE509_00071
LPM: LP6_2974
LPE: lp12_2928
MMOB: F6R98_01395
FTQ: RO31_0447
FTF: FTF0399c
FTW: FTW_1675
FTR: NE061598_02225
FTT: FTV_0370
FTG: FTU_0454
FTL: FTL_0465
FTH: FTH_0462
FTA: FTA_0492
FTS: F92_02525
FTI: FTS_0466
FTC: DA46_230
FTV: CH67_766
FTZ: CH68_500
FTM: FTM_0554
FTN: FTN_0495
FTX: AW25_1534
FTD: AS84_189
FTY: CH70_1568
FRC: KX01_827
ABO: ABO_1915
ADI: B5T_01121
APAC: S7S_13465
AXE: P40_05315
PNU: Pnuc_1246
PNE: Pnec_1110
PNA: Pnap_3075
SLT: Slit_0318
GCA: Galf_0202
DAR: Daro_0557
AOA: dqs_0821
BPRC: D521_0592
HPY: HP0580
HPJ: jhp_0527
HPG: HPG27_539
HPL: HPB8_776
HPZ: HPKB_0764
HPD: KHP_0738
HEY: MWE_0937
HPYR: K747_06970
HPYI: K750_05180
HPYU: K751_03520
HPYM: K749_01610
HEB: U063_0885
HEZ: U064_0889
HHE: HH_1672
HAC: Hac_1432
HMS: HMU03750
HCE: HCW_06235
HCM: HCD_01355
HCP: HCN_0866
CAVI: CAV_1281
HDN: Hden_0853
 » show all
Reference
  Authors
Stead CM, Zhao J, Raetz CR, Trent MS
  Title
Removal of the outer Kdo from Helicobacter pylori lipopolysaccharide and its impact on the bacterial surface.
  Journal
Mol Microbiol 78:837-52 (2010)
DOI:10.1111/j.1365-2958.2010.07304.x
  Sequence
[hpy:HP0580]

KEGG   ENZYME: 3.2.1.144
Entry
EC 3.2.1.144                Enzyme                                 

Name
3-deoxyoctulosonase;
alpha-Kdo-ase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
3-deoxyoctulosonyl-lipopolysaccharide hydrolase
Reaction(IUBMB)
3-deoxyoctulosonyl-lipopolysaccharide + H2O = 3-deoxyoctulosonic acid + lipopolysaccharide [RN:R06054]
Reaction(KEGG)
R06054;
(other) R12219
Substrate
3-deoxyoctulosonyl-lipopolysaccharide [CPD:C06744];
H2O [CPD:C00001]
Product
3-deoxyoctulosonic acid [CPD:C01187];
lipopolysaccharide [CPD:C00338]
Comment
Releases Kdo (alpha- and beta-linked 3-deoxy-D-manno-octulosonic acid) from different lipopolysaccharides, including Re-LPS from Escherichia coli and Salmonella, Rd-LPS from S. minnesota, and de-O-acyl-re-LPS. 4-Methylumbelliferyl-alpha-Kdo (alpha-Kdo-OMec) is also a substrate.
History
EC 3.2.1.144 created 2000
Pathway
ec00540  Lipopolysaccharide biosynthesis
ec01100  Metabolic pathways
Orthology
K23082  3-deoxy-D-manno-octulosonic acid-hydrolase
Genes
LPN: lpg2938
LPO: LPO_3263
LPU: LPE509_00072
LPM: LP6_2973
LPE: lp12_2927
UPI: EJG51_009680
HPY: HP0579
HEO: C694_02985
HPJ: jhp_0526
HPA: HPAG1_0558
HPG: HPG27_538
HPL: HPB8_775
HPZ: HPKB_0765
HPD: KHP_0739
HEY: MWE_0938
HPYR: K747_06965
HPYI: K750_05175
HPYU: K751_03515
HPYM: K749_01605
HEB: U063_0884
HEZ: U064_0888
HAC: Hac_1433
HMS: HMU03740
HCE: HCW_06240
HCM: HCD_01360
 » show all
Reference
1  [PMID:9334217]
  Authors
Li YT, Wang LX, Pavlova NV, Li SC, Lee YC.
  Title
alpha-KDOase activity in oyster and synthesis of alpha- and beta-4-methylumbelliferyl ketosides of 3-deoxy-D-manno-octulosonic acid.
  Journal
J Biol Chem 272:26419-24 (1997)
DOI:10.1074/jbc.272.42.26419
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.144
IUBMB Enzyme Nomenclature: 3.2.1.144
ExPASy - ENZYME nomenclature database: 3.2.1.144
BRENDA, the Enzyme Database: 3.2.1.144
CAS: 199128-67-1

KEGG   REACTION: R12219
Entry
R12219                      Reaction                               

Name
KDO2-lipid-A-1-(2-aminoethyl phosphate) hydrolase
Definition
1-PEtN-KDO2-lipid A + H2O <=> 1-PEtN-KDO-lipid A + 3-Deoxy-D-manno-octulosonate
Equation
Enzyme
Pathway
rn00540  Lipopolysaccharide biosynthesis
rn01100  Metabolic pathways
Module
M00867  KDO2-lipid A modification pathway
Orthology
K23082  3-deoxy-D-manno-octulosonic acid-hydrolase [EC:3.2.1.144]
K23083  3-deoxy-D-manno-octulosonic acid-hydrolase

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