KEGG   ORTHOLOGY: K23121
Entry
K23121                      KO                                     

Name
pflD
Definition
trans-4-hydroxy-L-proline dehydratase [EC:4.2.1.172]
Pathway
ko00330  Arginine and proline metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00330 Arginine and proline metabolism
    K23121  pflD; trans-4-hydroxy-L-proline dehydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.172  trans-4-hydroxy-L-proline dehydratase
     K23121  pflD; trans-4-hydroxy-L-proline dehydratase
Other DBs
RN: R11819
COG: COG1882
Genes
BNG: EH206_07645
GSU: GSU2101
GSK: KN400_2127
GBM: Gbem_2499
GEB: GM18_2359
DDE: Dde_3055
DDN: DND132_1582
DBA: Dbac_2366
DPS: DP0616(pflD)
DEO: CAY53_00670
DAT: HRM2_04250(pflD1) HRM2_49330(pflD2)
DTO: TOL2_C32550(pflD)
RVA: Rvan_3601
CBO: CBO3183
CBA: CLB_3219
CBH: CLC_3093
CBY: CLM_3595
CBL: CLK_2581
CBB: CLD_1348
CBI: CLJ_B3455
CBF: CLI_3322
CBM: CBF_3314
CKL: CKL_0323
CKR: CKR_0275
CPAS: Clopa_4023
CSQ: CSCA_0866
CLD: CLSPO_c32860(pflD2)
CACE: CACET_c25680(pflD2)
CTYK: CTK_C05150
AMT: Amet_0584
HHW: NCTC503_02347(csdB_2)
BPRO: PMF13cell1_04098(cutC_3)
CSCI: HDCHBGLK_00253(cutC_1)
CSO: CLS_03180
CDF: CD630_32820(pflD)
CDC: CD196_3097(pflD)
CDL: CDR20291_3143(pflD)
PDF: CD630DERM_32820(pflD)
PHX: KGNDJEFE_00506(cutC_2)
DRM: Dred_2965
PTH: PTH_2436(PflD)
SGY: Sgly_2533
AWO: Awo_c14930(pflB2)
OVA: OBV_40980
CTHM: CFE_0574
BPRM: CL3_28280
PUF: UFO1_0399
PFT: JBW_04226
MANA: MAMMFC1_02761(bssA_3)
STED: SPTER_40410(cutC_6)
EUC: EC1_17640
AMUC: Pan181_08370(bssA)
PBU: L21SP3_00214(csdB)
PBP: STSP1_00751(csdB)
ALUS: STSP2_03489(bssA)
TAI: Taci_1437
TLI: Tlie_1574
BVU: BVU_1421
PSAC: PSM36_1174
PDI: BDI_0786
DORI: FH5T_08275
BLQ: L21SP5_00958(bssA)
MBAS: ALGA_0749
TLE: Tlet_1141
METC: MTCT_0292
TKO: TK0289
TSI: TSIB_0631
TLT: OCC_00382
ABI: Aboo_0169
KCR: Kcr_0517
LOKI: Lokiarch_12750(bssA_1)
 » show all
Reference
  Authors
Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP
  Title
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.
  Journal
Science 355:eaai8386 (2017)
DOI:10.1126/science.aai8386
  Sequence

KEGG   ENZYME: 4.2.1.172
Entry
EC 4.2.1.172                Enzyme                                 

Name
trans-4-hydroxy-L-proline dehydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
trans-4-hydroxy-L-proline hydro-lyase
Reaction(IUBMB)
trans-4-hydroxy-L-proline = (S)-1-pyrroline-5-carboxylate + H2O [RN:R11819]
Reaction(KEGG)
R11819
Substrate
trans-4-hydroxy-L-proline [CPD:C01157]
Product
(S)-1-pyrroline-5-carboxylate [CPD:C03912];
H2O [CPD:C00001]
Comment
The enzyme has been characterized from the bacterium Peptoclostridium difficile. The active form contains a glycyl radical that is generated by a dedicated activating enzyme via chemistry involving S-adenosyl-L-methionine (SAM) and a [4Fe-4S] cluster.
History
EC 4.2.1.172 created 2017
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
Orthology
K23121  trans-4-hydroxy-L-proline dehydratase
Genes
BNG: EH206_07645
GSU: GSU2101
GSK: KN400_2127
GBM: Gbem_2499
GEB: GM18_2359
DDE: Dde_3055
DDN: DND132_1582
DBA: Dbac_2366
DPS: DP0616(pflD)
DEO: CAY53_00670
DAT: HRM2_04250(pflD1) HRM2_49330(pflD2)
DTO: TOL2_C32550(pflD)
RVA: Rvan_3601
CBO: CBO3183
CBA: CLB_3219
CBH: CLC_3093
CBY: CLM_3595
CBL: CLK_2581
CBB: CLD_1348
CBI: CLJ_B3455
CBF: CLI_3322
CBM: CBF_3314
CKL: CKL_0323
CKR: CKR_0275
CPAS: Clopa_4023
CSQ: CSCA_0866
CLD: CLSPO_c32860(pflD2)
CACE: CACET_c25680(pflD2)
CTYK: CTK_C05150
AMT: Amet_0584
HHW: NCTC503_02347(csdB_2)
BPRO: PMF13cell1_04098(cutC_3)
CSCI: HDCHBGLK_00253(cutC_1)
CSO: CLS_03180
CDF: CD630_32820(pflD)
CDC: CD196_3097(pflD)
CDL: CDR20291_3143(pflD)
PDF: CD630DERM_32820(pflD)
PHX: KGNDJEFE_00506(cutC_2)
DRM: Dred_2965
PTH: PTH_2436(PflD)
SGY: Sgly_2533
AWO: Awo_c14930(pflB2)
OVA: OBV_40980
CTHM: CFE_0574
BPRM: CL3_28280
PUF: UFO1_0399
PFT: JBW_04226
MANA: MAMMFC1_02761(bssA_3)
STED: SPTER_40410(cutC_6)
EUC: EC1_17640
AMUC: Pan181_08370(bssA)
PBU: L21SP3_00214(csdB)
PBP: STSP1_00751(csdB)
ALUS: STSP2_03489(bssA)
TAI: Taci_1437
TLI: Tlie_1574
BVU: BVU_1421
PSAC: PSM36_1174
PDI: BDI_0786
DORI: FH5T_08275
BLQ: L21SP5_00958(bssA)
MBAS: ALGA_0749
TLE: Tlet_1141
METC: MTCT_0292
TKO: TK0289
TSI: TSIB_0631
TLT: OCC_00382
ABI: Aboo_0169
KCR: Kcr_0517
LOKI: Lokiarch_12750(bssA_1)
 » show all
Reference
1  [PMID:28183913]
  Authors
Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP
  Title
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.
  Journal
Science 355:eaai8386 (2017)
DOI:10.1126/science.aai8386
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.172
IUBMB Enzyme Nomenclature: 4.2.1.172
ExPASy - ENZYME nomenclature database: 4.2.1.172
BRENDA, the Enzyme Database: 4.2.1.172

KEGG   REACTION: R11819
Entry
R11819                      Reaction                               

Name
trans-4-hydroxy-L-proline hydro-lyase
Definition
Hydroxyproline <=> (S)-1-Pyrroline-5-carboxylate + H2O
Equation
Reaction class
RC03501  C01157_C03912
Enzyme
Pathway
rn00330  Arginine and proline metabolism
rn01100  Metabolic pathways
Orthology
K23121  trans-4-hydroxy-L-proline dehydratase [EC:4.2.1.172]
Other DBs
RHEA: 54647

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