KEGG   ORTHOLOGY: K23374
Entry
K23374                      KO                                     

Name
sbnF
Definition
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase [EC:6.3.2.55]
Pathway
ko00997  Biosynthesis of various secondary metabolites - part 3
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00875  Staphyloferrin B biosynthesis, L-serine => staphyloferrin B
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various secondary metabolites - part 3
    K23374  sbnF; 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.55  2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase
     K23374  sbnF; 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase
Other DBs
RN: R12311
COG: COG4264
Genes
SGL: SGP1_0041
DDD: Dda3937_02851(acsC)
DZE: Dd1591_2674
DDC: Dd586_2581
DZC: W909_07420
DSO: A4U42_16815
CED: LH89_04360
DFN: CVE23_08420
DDQ: DDI_1277
DAQ: DAQ1742_01514(acsC)
XDO: XDD1_1966
VTA: A3470
PSB: Psyr_2587
PSYR: N018_14375
PSEM: TO66_16020
PSIL: PMA3_09135
PALI: A3K91_1226
ABY: ABAYE2005
ABN: AB57_1872
ABB: ABBFA_01825(iucC_2)
ABX: ABK1_2133
ABH: M3Q_2027
ABAD: ABD1_16350
ABAZ: P795_9035
ABAU: IX87_19070
ABAA: IX88_10155
ACI: ACIAD2121
HEL: HELO_3334
RSO: RSp0422
RSN: RSPO_m01406(rhbC)
RSE: F504_3902(sbnF)
RSY: RSUY_34900(iucC_2)
CNC: CNE_2c14880(sbnA2)
RME: Rmet_1112
NET: Neut_1745
BID: Bind_2101
SAU: SA0117(sbnF)
SAV: SAV0121
SAW: SAHV_0120
SAM: MW0094(sbnF)
SAS: SAS0095
SAR: SAR0124
SAC: SACOL0105
SAE: NWMN_0065(sbnF)
SAD: SAAV_0089
SUU: M013TW_0111(sbnF)
SUE: SAOV_0068
SUC: ECTR2_77
SUZ: MS7_0113
SUW: SATW20_01330(sbnF)
SUG: SAPIG0133
SUF: SARLGA251_00960(sbnF)
SAUA: SAAG_00605
SAUS: SA40_0088(sbnF)
SAUU: SA957_0103(sbnF)
SAUG: SA268_0100(sbnF)
SAUZ: SAZ172_0132(sbnF)
SAUF: X998_0100
SAB: SAB0060
SUY: SA2981_0122(sbnF)
SAUB: C248_0109
SAUM: BN843_1230
SAUC: CA347_132
SAUR: SABB_03469
SAUI: AZ30_00630
SAUD: CH52_05115
SAMS: NI36_00525
SDT: SPSE_2119
SHU: SHYC_00380(sbnF)
SSCH: LH95_00380
SSCZ: RN70_00600
SAGQ: EP23_09745(sbnF)
SFF: FOB90_07410(sbnF)
SDP: NCTC12225_02336(iucC_3)
PPOL: X809_04490
 » show all
Reference
  Authors
Dale SE, Doherty-Kirby A, Lajoie G, Heinrichs DE
  Title
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
  Journal
Infect Immun 72:29-37 (2004)
DOI:10.1128/IAI.72.1.29-37.2004
  Sequence
[sam:MW0094]
Reference
  Authors
Cheung J, Beasley FC, Liu S, Lajoie GA, Heinrichs DE
  Title
Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus.
  Journal
Mol Microbiol 74:594-608 (2009)
DOI:10.1111/j.1365-2958.2009.06880.x

KEGG   ENZYME: 6.3.2.55
Entry
EC 6.3.2.55                 Enzyme                                 

Name
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase;
sbnF (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate:L-2,3-diaminopropanoate ligase {2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate-forming}
Reaction(IUBMB)
ATP + 2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate + L-2,3-diaminopropanoate = AMP + diphosphate + 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate [RN:R12311]
Reaction(KEGG)
R12311
Substrate
ATP [CPD:C00002];
2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate [CPD:C22073];
L-2,3-diaminopropanoate [CPD:C03401]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate [CPD:C22074]
Comment
Requires Mg2+. The enzyme, characterized from the bacterium Staphylococcus aureus, is involved in the biosynthesis of the siderophore staphyloferrin B. It belongs to a class of siderophore synthases known as type C nonribosomal peptide synthase-independent synthases (NIS). Type C NIS enzymes recognize esterified or amidated derivatives of carboxylic acids. The enzyme likely forms a 2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate adenylate intermediate prior to ligation.
History
EC 6.3.2.55 created 2019
Pathway
ec00997  Biosynthesis of various secondary metabolites - part 3
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23374  2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase
Genes
SGL: SGP1_0041
DDD: Dda3937_02851(acsC)
DZE: Dd1591_2674
DDC: Dd586_2581
DZC: W909_07420
DSO: A4U42_16815
CED: LH89_04360
DFN: CVE23_08420
DDQ: DDI_1277
DAQ: DAQ1742_01514(acsC)
XDO: XDD1_1966
VTA: A3470
PSB: Psyr_2587
PSYR: N018_14375
PSEM: TO66_16020
PSIL: PMA3_09135
PALI: A3K91_1226
ABY: ABAYE2005
ABN: AB57_1872
ABB: ABBFA_01825(iucC_2)
ABX: ABK1_2133
ABH: M3Q_2027
ABAD: ABD1_16350
ABAZ: P795_9035
ABAU: IX87_19070
ABAA: IX88_10155
ACI: ACIAD2121
HEL: HELO_3334
RSO: RSp0422
RSN: RSPO_m01406(rhbC)
RSE: F504_3902(sbnF)
RSY: RSUY_34900(iucC_2)
CNC: CNE_2c14880(sbnA2)
RME: Rmet_1112
NET: Neut_1745
BID: Bind_2101
SAU: SA0117(sbnF)
SAV: SAV0121
SAW: SAHV_0120
SAM: MW0094(sbnF)
SAS: SAS0095
SAR: SAR0124
SAC: SACOL0105
SAE: NWMN_0065(sbnF)
SAD: SAAV_0089
SUU: M013TW_0111(sbnF)
SUE: SAOV_0068
SUC: ECTR2_77
SUZ: MS7_0113
SUW: SATW20_01330(sbnF)
SUG: SAPIG0133
SUF: SARLGA251_00960(sbnF)
SAUA: SAAG_00605
SAUS: SA40_0088(sbnF)
SAUU: SA957_0103(sbnF)
SAUG: SA268_0100(sbnF)
SAUZ: SAZ172_0132(sbnF)
SAUF: X998_0100
SAB: SAB0060
SUY: SA2981_0122(sbnF)
SAUB: C248_0109
SAUM: BN843_1230
SAUC: CA347_132
SAUR: SABB_03469
SAUI: AZ30_00630
SAUD: CH52_05115
SAMS: NI36_00525
SDT: SPSE_2119
SHU: SHYC_00380(sbnF)
SSCH: LH95_00380
SSCZ: RN70_00600
SAGQ: EP23_09745(sbnF)
SFF: FOB90_07410(sbnF)
SDP: NCTC12225_02336(iucC_3)
PPOL: X809_04490
 » show all
Reference
1  [PMID:19775248]
  Authors
Cheung J, Beasley FC, Liu S, Lajoie GA, Heinrichs DE
  Title
Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus.
  Journal
Mol Microbiol 74:594-608 (2009)
DOI:10.1111/j.1365-2958.2009.06880.x
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.55
IUBMB Enzyme Nomenclature: 6.3.2.55
ExPASy - ENZYME nomenclature database: 6.3.2.55
BRENDA, the Enzyme Database: 6.3.2.55

KEGG   REACTION: R12311
Entry
R12311                      Reaction                               

Name
2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate:L-2,3-diaminopropanoate ligase {2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate-forming}
Definition
ATP + 2-[(2-Aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate + L-2,3-Diaminopropanoate <=> AMP + Diphosphate + 2-[(L-Alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate
Equation
Enzyme
Pathway
rn00997  Biosynthesis of various secondary metabolites - part 3
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00875  Staphyloferrin B biosynthesis, L-serine => staphyloferrin B
Orthology
K23374  2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase [EC:6.3.2.55]

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