KEGG   ORTHOLOGY: K23999
Entry
K23999                      KO                                     

Name
fom1
Definition
phosphoenolpyruvate phosphomutase / 2-hydroxyethylphosphonate cytidylyltransferase [EC:5.4.2.9 2.7.7.104]
Pathway
ko00440  Phosphonate and phosphinate metabolism
ko00998  Biosynthesis of various secondary metabolites - part 2
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00903  Fosfomycin biosynthesis, phosphoenolpyruvate => fosfomycin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00440 Phosphonate and phosphinate metabolism
    K23999  fom1; phosphoenolpyruvate phosphomutase / 2-hydroxyethylphosphonate cytidylyltransferase
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various secondary metabolites - part 2
    K23999  fom1; phosphoenolpyruvate phosphomutase / 2-hydroxyethylphosphonate cytidylyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.7  Transferring phosphorus-containing groups
   2.7.7  Nucleotidyltransferases
    2.7.7.104  2-hydroxyethylphosphonate cytidylyltransferase
     K23999  fom1; phosphoenolpyruvate phosphomutase / 2-hydroxyethylphosphonate cytidylyltransferase
 5. Isomerases
  5.4  Intramolecular transferases
   5.4.2  Phosphotransferases (phosphomutases)
    5.4.2.9  phosphoenolpyruvate mutase
     K23999  fom1; phosphoenolpyruvate phosphomutase / 2-hydroxyethylphosphonate cytidylyltransferase
Other DBs
RN: R00661 R12498
COG: COG2513 COG0615
GO: 0050188
Genes
OSG: BST96_06905
PHN: PAEH1_00300
MEU: ACJ67_08960
AZO: azo2698(pepM)
AOA: dqs_2833
HCP: HCN_1062
HCB: HCBAA847_0912
CHYO: CHH_1593
CHW: A2J15_004815(aepX)
ANT: Arnit_2943
DAL: Dalk_1323
ECOG: FIV45_08000(aepX)
CTC: CTC_01698
CBE: Cbei_4338
CBZ: Cbs_4338
CBEI: LF65_04862
CSR: Cspa_c46190(ppm)
CSB: CLSA_c08730(ppm)
CDY: F3K33_22185(aepX)
RIX: RO1_19910
SNA: Snas_5665
OLS: Olsu_0594
TDE: TDE1413
TPHG: FUT81_06855(aepX)
FSC: FSU_1439
BTH: BT_1720
BTHO: Btheta7330_01295(pphA)
BFS: BF9343_1814(aepX)
DRC: G0Q07_19260(aepX)
PSYT: DSAG12_01622(ribL_1)
AG: BAA32495(fom1)
 » show all
Reference
  Authors
Cho SH, Kim SY, Tomita T, Shiraishi T, Park JS, Sato S, Kudo F, Eguchi T, Funa N, Nishiyama M, Kuzuyama T
  Title
Fosfomycin Biosynthesis via Transient Cytidylylation of 2-Hydroxyethylphosphonate by the Bifunctional Fom1 Enzyme.
  Journal
ACS Chem Biol 12:2209-2215 (2017)
DOI:10.1021/acschembio.7b00419
  Sequence

KEGG   ENZYME: 2.7.7.104
Entry
EC 2.7.7.104                Enzyme                                 

Name
2-hydroxyethylphosphonate cytidylyltransferase;
Fom1
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
Sysname
CTP:2-hydroxyethylphosphonate cytidylyltransferase
Reaction(IUBMB)
2-hydroxyethylphosphonate + CTP = cytidine 5'-{[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate} + diphosphate
Substrate
2-hydroxyethylphosphonate [CPD:C06451];
CTP [CPD:C00063]
Product
cytidine 5'-{[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate} [CPD:C22216];
diphosphate [CPD:C00013]
Comment
The enzyme, isolated from the bacterium Streptomyces wedmorensis, is involved in fosfomycin biosynthesis. The enzyme also is active as EC 5.4.2.9 phosphoenolpyruvate mutase.
History
EC 2.7.7.104 created 2020
Pathway
ec00998  Biosynthesis of various secondary metabolites - part 2
ec01100  Metabolic pathways
Orthology
K23999  phosphoenolpyruvate phosphomutase / 2-hydroxyethylphosphonate cytidylyltransferase
Genes
OSG: BST96_06905
PHN: PAEH1_00300
MEU: ACJ67_08960
AZO: azo2698(pepM)
AOA: dqs_2833
HCP: HCN_1062
HCB: HCBAA847_0912
CHYO: CHH_1593
CHW: A2J15_004815(aepX)
ANT: Arnit_2943
DAL: Dalk_1323
ECOG: FIV45_08000(aepX)
CTC: CTC_01698
CBE: Cbei_4338
CBZ: Cbs_4338
CBEI: LF65_04862
CSR: Cspa_c46190(ppm)
CSB: CLSA_c08730(ppm)
CDY: F3K33_22185(aepX)
RIX: RO1_19910
SNA: Snas_5665
OLS: Olsu_0594
TDE: TDE1413
TPHG: FUT81_06855(aepX)
FSC: FSU_1439
BTH: BT_1720
BTHO: Btheta7330_01295(pphA)
BFS: BF9343_1814(aepX)
DRC: G0Q07_19260(aepX)
PSYT: DSAG12_01622(ribL_1)
 » show all
Reference
1  [PMID:28727444]
  Authors
Cho SH, Kim SY, Tomita T, Shiraishi T, Park JS, Sato S, Kudo F, Eguchi T, Funa N, Nishiyama M, Kuzuyama T
  Title
Fosfomycin Biosynthesis via Transient Cytidylylation of 2-Hydroxyethylphosphonate by the Bifunctional Fom1 Enzyme.
  Journal
ACS Chem Biol 12:2209-2215 (2017)
DOI:10.1021/acschembio.7b00419
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.104
IUBMB Enzyme Nomenclature: 2.7.7.104
ExPASy - ENZYME nomenclature database: 2.7.7.104
BRENDA, the Enzyme Database: 2.7.7.104

KEGG   REACTION: R12498
Entry
R12498                      Reaction                               

Definition
2-Hydroxyethylphosphonate + CTP <=> Cytidine 5'-{[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate} + Diphosphate
Equation
Comment
cytidylyltransferase
Reaction class
RC00002  C06451_C22216
Pathway
rn00998  Biosynthesis of various secondary metabolites - part 2
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00903  Fosfomycin biosynthesis, phosphoenolpyruvate => fosfomycin
Orthology
K23999  phosphoenolpyruvate phosphomutase / 2-hydroxyethylphosphonate cytidylyltransferase [EC:5.4.2.9 2.7.7.104]

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