KEGG   REACTION: R00918
Entry
R00918                      Reaction                               

Name
propanoyl-CoA:(2S)-methylmalonyl-CoA malonyltransferase (cyclizing);
propanoyl-CoA:(2S)-methylmalonyl-CoA 2-C-acyltransferase (decarboxylating, oxoacyl-reducing and cyclizing)
Definition
Propanoyl-CoA + 6 (S)-Methylmalonyl-CoA + 6 NADPH + 6 H+ <=> 6-Deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
Equation
C00100 + 6 C00683 + 6 C00005 + 6 C00080 <=> C03240 + 7 C00010 + 6 C00011 + C00001 + 6 C00006
Comment
EryA MegA
multi-step reaction
Reaction class
RC00001  C00005_C00006
RC00004  C00010_C00100  C00010_C00683
RC02825  C00100_C03240
RC02826  C00683_C03240
RC02828  C00683_C03240
RC02829  C00683_C03240
RC02830  C00683_C03240
RC02831  C00683_C03240
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Orthology
K10817  6-deoxyerythronolide-B synthase EryAI [EC:2.3.1.94]
K24563  6-deoxyerythronolide B synthase EryAII [EC:2.3.1.94]
K24564  6-deoxyerythronolide B synthase EryAIII [EC:2.3.1.94]
Reference
1  [PMID:11752428]
  Authors
Tsai SC, Miercke LJ, Krucinski J, Gokhale R, Chen JC, Foster PG, Cane DE, Khosla C, Stroud RM
  Title
Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel.
  Journal
Proc Natl Acad Sci U S A 98:14808-13 (2001)
DOI:10.1073/pnas.011399198
Other DBs
RHEA: 23071

KEGG   RCLASS: RC02825
Entry
RC02825                     RClass                                 

Definition
C1y-C5a:C1y+*-*+S2a:C1b+O7x-C1b+O5a
O7x-O5a:C7x-*:C1y-C5a
Reactant pair
Related class
Reaction
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
Orthology
K10817  6-deoxyerythronolide-B synthase EryAI [EC:2.3.1.94]
K24563  6-deoxyerythronolide B synthase EryAII [EC:2.3.1.94]
K24564  6-deoxyerythronolide B synthase EryAIII [EC:2.3.1.94]

KEGG   RCLASS: RC02826
Entry
RC02826                     RClass                                 

Definition
C1c-C1y:C6a+*-*+C1y:C1a+C5a-C1a+C1y
C5a-C1y:S2a+*-*+C1y:C1c+O5a-C1y+O1a
Reactant pair
Related class
Reaction
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K10817  6-deoxyerythronolide-B synthase EryAI [EC:2.3.1.94]
K16007  8,8a-deoxyoleandolide synthase 1
K24563  6-deoxyerythronolide B synthase EryAII [EC:2.3.1.94]
K24564  6-deoxyerythronolide B synthase EryAIII [EC:2.3.1.94]
K24565  8,8a-deoxyoleandolide synthase 2
K24566  8,8a-deoxyoleandolide synthase 3

KEGG   RCLASS: RC02828
Entry
RC02828                     RClass                                 

Definition
C1c-C1y:C6a+*-*+C1y:C1a+C5a-C1a+C5x
C5a-C5x:S2a+*-*+C1y:C1c+O5a-C1y+O5x
Reactant pair
Related class
Reaction
Enzyme
2.3.1.94        2.3.1.240
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K10817  6-deoxyerythronolide-B synthase EryAI [EC:2.3.1.94]
K16000  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16001  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16002  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16003  narbonolide synthase [EC:2.3.1.240]
K16007  8,8a-deoxyoleandolide synthase 1
K24563  6-deoxyerythronolide B synthase EryAII [EC:2.3.1.94]
K24564  6-deoxyerythronolide B synthase EryAIII [EC:2.3.1.94]
K24565  8,8a-deoxyoleandolide synthase 2
K24566  8,8a-deoxyoleandolide synthase 3

KEGG   RCLASS: RC02829
Entry
RC02829                     RClass                                 

Definition
C1c-C1y:C6a+*-*+C5x:C1a+C5a-C1a+C1x
C5a-C1x:O5a+S2a+*-*+*+C1y:C1c-C1y
Reactant pair
Related class
Reaction
Enzyme
2.3.1.94        2.3.1.239       2.3.1.240
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K10817  6-deoxyerythronolide-B synthase EryAI [EC:2.3.1.94]
K15988  tylactone synthase 1
K16000  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16001  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16002  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16003  narbonolide synthase [EC:2.3.1.240]
K16007  8,8a-deoxyoleandolide synthase 1
K24555  tylactone synthase 2
K24556  tylactone synthase 3
K24557  tylactone synthase 4
K24558  tylactone synthase 5
K24563  6-deoxyerythronolide B synthase EryAII [EC:2.3.1.94]
K24564  6-deoxyerythronolide B synthase EryAIII [EC:2.3.1.94]
K24565  8,8a-deoxyoleandolide synthase 2
K24566  8,8a-deoxyoleandolide synthase 3

KEGG   RCLASS: RC02830
Entry
RC02830                     RClass                                 

Definition
C1c-C1y:C6a+*-*+C1x:C1a+C5a-C1a+C1y
C5a-C1y:S2a+*-*+C1y:C1c+O5a-C1y+O1a
Reactant pair
Related class
Reaction
Enzyme
2.3.1.94        2.3.1.239       2.3.1.240
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K10817  6-deoxyerythronolide-B synthase EryAI [EC:2.3.1.94]
K16000  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16001  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16002  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16003  narbonolide synthase [EC:2.3.1.240]
K16007  8,8a-deoxyoleandolide synthase 1
K24563  6-deoxyerythronolide B synthase EryAII [EC:2.3.1.94]
K24564  6-deoxyerythronolide B synthase EryAIII [EC:2.3.1.94]
K24565  8,8a-deoxyoleandolide synthase 2
K24566  8,8a-deoxyoleandolide synthase 3

KEGG   RCLASS: RC02831
Entry
RC02831                     RClass                                 

Definition
C1c-C1y:C6a+*-*+C1y:C1a+C5a-C1a+C7x
C5a-C7x:S2a+*-*+O7x:C1c+O5a-C1y+O6a
Reactant pair
Related class
Reaction
Enzyme
2.3.1.94        2.3.1.239
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K10817  6-deoxyerythronolide-B synthase EryAI [EC:2.3.1.94]
K16000  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16001  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16002  narbonolide/10-deoxymethynolide synthase [EC:2.3.1.240 2.3.1.239]
K16007  8,8a-deoxyoleandolide synthase 1
K24563  6-deoxyerythronolide B synthase EryAII [EC:2.3.1.94]
K24564  6-deoxyerythronolide B synthase EryAIII [EC:2.3.1.94]
K24565  8,8a-deoxyoleandolide synthase 2
K24566  8,8a-deoxyoleandolide synthase 3

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