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Entry
bsu03030                    Pathway                                

Name
DNA replication - Bacillus subtilis subsp. subtilis 168
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
BRITE hierarchy
Pathway map
bsu03030  DNA replication
bsu03030

Ortholog table
Module
bsu_M00260  DNA polymerase III complex, bacteria [PATH:bsu03030]
Organism
Bacillus subtilis subsp. subtilis 168 [GN:bsu]
Gene
BSU29230  dnaE; DNA polymerase III subunit alpha [KO:K02337] [EC:2.7.7.7]
BSU20340  yorL; DNA polymerase YorL [KO:K02337] [EC:2.7.7.7]
BSU16580  polC; DNA polymerase III PolC-type [KO:K03763] [EC:2.7.7.7]
BSU00190  dnaX; DNA polymerase III subunit gamma/tau [KO:K02343] [EC:2.7.7.7]
BSU00310  holB; DNA polymerase III subunit delta [KO:K02341] [EC:2.7.7.7]
BSU25560  holA; hypothetical protein [KO:K02340] [EC:2.7.7.7]
BSU00020  dnaN; DNA polymerase III subunit beta [KO:K02338] [EC:2.7.7.7]
BSU40440  dnaC; replicative DNA helicase [KO:K02314] [EC:3.6.4.12]
BSU25210  dnaG; DNA primase [KO:K02316] [EC:2.7.7.101]
BSU40900  ssbA; single-stranded DNA-binding protein [KO:K03111]
BSU21990  ypdQ; ribonuclease H-like protein [KO:K03469] [EC:3.1.26.4]
BSU21970  ypeP; hypothetical protein [KO:K03469] [EC:3.1.26.4]
BSU16060  rnhB; ribonuclease HII [KO:K03470] [EC:3.1.26.4]
BSU28620  rnhC; ribonuclease HIII [KO:K03471] [EC:3.1.26.4]
BSU29090  polA; DNA polymerase I [KO:K02335] [EC:2.7.7.7]
BSU06620  ligA; DNA ligase [KO:K01972] [EC:6.5.1.2]
BSU20500  ligB; DNA ligase-like protein LigB [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   

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