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cel03030                    Pathway                                

DNA replication - Caenorhabditis elegans (nematode)
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
09120 Genetic Information Processing; 09124 Replication and repair
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Pathway map
cel03030  DNA replication

Ortholog table
cel_M00261  DNA polymerase alpha / primase complex [PATH:cel03030]
cel_M00263  DNA polymerase epsilon complex [PATH:cel03030]
cel_M00285  MCM complex [PATH:cel03030]
cel_M00289  RF-C complex [PATH:cel03030]
Caenorhabditis elegans (nematode) [GN:cel]
CELE_C04F12.9  rnh-1.3; RNase H [KO:K03469] [EC:]
CELE_F59A6.6  rnh-1.0; RNase H [KO:K03469] [EC:]
CELE_F18A1.5  rpa-1; Probable replication factor A 73 kDa subunit [KO:K07466]
CELE_W03D2.4  pcn-1; Proliferating cell nuclear antigen [KO:K04802]
CELE_F43G6.1  dna-2; yeast DNA helicase/endonuclease family [KO:K10742] [EC:]
CELE_Y47G6A.8  crn-1; Flap endonuclease 1 [KO:K04799] [EC:3.-.-.-]
CELE_C29A12.3  lig-1; DNA ligase 1 [KO:K10747] [EC:]
CELE_Y47D3A.29  pola-1; DNA polymerase [KO:K02320] [EC:]
CELE_R01H10.1  div-1; DNA polymerase alpha subunit B [KO:K02321]
CELE_T24C4.5  T24C4.5; DNA primase [KO:K02684] [EC:2.7.7.-]
CELE_F58A4.4  pri-1; DNA primase small subunit [KO:K02684] [EC:2.7.7.-]
CELE_W02D9.1  pri-2; DNA primase large subunit [KO:K02685] [EC:2.7.7.-]
CELE_F10C2.4  F10C2.4; DNA polymerase delta catalytic subunit [KO:K02327] [EC:]
CELE_F12F6.7  F12F6.7; Probable DNA polymerase delta small subunit [KO:K02328]
CELE_R04F11.3  R04F11.3; Uncharacterized protein [KO:K03504]
CELE_F33H2.5  pole-1; DNA polymerase [KO:K02324] [EC:]
CELE_F08B4.5  pole-2; Probable DNA polymerase epsilon subunit 2 [KO:K02325] [EC:]
CELE_T26A5.8  T26A5.8; Uncharacterized protein [KO:K02326] [EC:]
CELE_Y53F4B.3  Y53F4B.3; Uncharacterized protein [KO:K03506] [EC:]
CELE_Y17G7B.5  mcm-2; DNA helicase [KO:K02540] [EC:]
CELE_C25D7.6  mcm-3; DNA helicase [KO:K02541] [EC:]
CELE_Y39G10AR.14  mcm-4; DNA replication licensing factor mcm-4 [KO:K02212] [EC:]
CELE_R10E4.4  mcm-5; DNA replication licensing factor mcm-5 [KO:K02209] [EC:]
CELE_ZK632.1  mcm-6; DNA replication licensing factor mcm-6 [KO:K02542] [EC:]
CELE_F32D1.10  mcm-7; DNA helicase [KO:K02210] [EC:]
CELE_M04F3.1  rpa-2; Replication Protein A homolog [KO:K10739]
CELE_F59A3.6  rpa-4; Replication Protein A homolog [KO:K10739]
CELE_C54G10.2  rfc-1; Replication factor C subunit 1 [KO:K10754]
CELE_F31E3.3  rfc-4; Replication factor C subunit 4 [KO:K10755]
CELE_F58F6.4  rfc-2; RFC (DNA replication factor) family [KO:K10755]
CELE_F44B9.8  F44B9.8; Probable replication factor C subunit 5 [KO:K10756]
CELE_C39E9.13  rfc-3; RFC (DNA replication factor) family [KO:K10756]
CELE_T13H5.7  rnh-2; Ribonuclease H2 subunit A [KO:K10743] [EC:]
CELE_F21D5.6  F21D5.6; Uncharacterized protein [KO:K10744]
CELE_ZK1127.13  ZK1127.13; Uncharacterized protein [KO:K10745]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
KO pathway

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