KEGG   PATHWAY: dme03030Help
Entry
dme03030                    Pathway                                

Name
DNA replication - Drosophila melanogaster (fruit fly)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
09120 Genetic Information Processing; 09124 Replication and repair
BRITE hierarchy
Pathway map
dme03030  DNA replication
dme03030

Ortholog table
Module
dme_M00261  DNA polymerase alpha / primase complex [PATH:dme03030]
dme_M00263  DNA polymerase epsilon complex [PATH:dme03030]
dme_M00285  MCM complex [PATH:dme03030]
dme_M00288  RPA complex [PATH:dme03030]
dme_M00289  RF-C complex [PATH:dme03030]
Organism
Drosophila melanogaster (fruit fly) [GN:dme]
Gene
Dmel_CG4337  mtSSB; mitochondrial single stranded DNA-binding protein [KO:K03111]
Dmel_CG8729  rnh1; ribonuclease H1, isoform A [KO:K03469] [EC:3.1.26.4]
Dmel_CG9633  RpA-70; replication protein A 70 [KO:K07466]
Dmel_CG9193  PCNA; proliferating cell nuclear antigen, isoform A [KO:K04802]
Dmel_CG10262  CG10262; uncharacterized protein [KO:K04802]
Dmel_CG2990  CG2990, isoform C [KO:K10742] [EC:3.6.4.12]
Dmel_CG8648  Fen1; flap endonuclease 1 [KO:K04799] [EC:3.-.-.-]
Dmel_CG5602  DNA-ligI; DNA ligase I [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
Dmel_CG6349  DNApol-alpha180; DNA polymerase alpha 180kD [KO:K02320] [EC:2.7.7.7]
Dmel_CG5923  DNApol-alpha73; DNA polymerase alpha 73kD [KO:K02321]
Dmel_CG7108  DNApol-alpha50; DNA polymerase alpha 50kD [KO:K02684] [EC:2.7.7.-]
Dmel_CG5553  DNApol-alpha60; DNA polymerase alpha 60kD [KO:K02685] [EC:2.7.7.-]
Dmel_CG5949  DNApol-delta; DNA-polymerase-delta [KO:K02327] [EC:2.7.7.7]
Dmel_CG12018  CG12018; uncharacterized protein [KO:K02328]
Dmel_CG3975  Pol32; Pol32 polymerase delta subunit, isoform B [KO:K03504]
Dmel_CG6768  DNApol-epsilon255; DNA polymerase epsilon 255kD subunit [KO:K02324] [EC:2.7.7.7]
Dmel_CG10489  DNApol-epsilon58; DNA polymerase epsilon 58kD subunit [KO:K02325] [EC:2.7.7.7]
Dmel_CG13399  Chrac-14; chromatin accessibility complex 14kD protein [KO:K02326] [EC:2.7.7.7]
Dmel_CG11301  Mes4; Mesoderm-expressed 4, isoform B [KO:K03506] [EC:2.7.7.7]
Dmel_CG7538  Mcm2; minichromosome maintenance 2 [KO:K02540] [EC:3.6.4.12]
Dmel_CG4206  Mcm3; minichromosome maintenance 3, isoform A [KO:K02541] [EC:3.6.4.12]
Dmel_CG1616  dpa; disc proliferation abnormal, isoform B [KO:K02212] [EC:3.6.4.12]
Dmel_CG4082  Mcm5; minichromosome maintenance 5 [KO:K02209] [EC:3.6.4.12]
Dmel_CG4039  Mcm6; minichromosome maintenance 6 [KO:K02542] [EC:3.6.4.12]
Dmel_CG4978  Mcm7; minichromosome maintenance 7 [KO:K02210] [EC:3.6.4.12]
Dmel_CG9273  RPA2; replication protein A2, isoform B [KO:K10739]
Dmel_CG15220  RPA3; replication protein A3, isoform B [KO:K10740]
Dmel_CG1119  Gnf1; germ line transcription factor 1, isoform A [KO:K10754]
Dmel_CG8142  CG8142 [KO:K10755]
Dmel_CG14999  RfC4; replication factor C subunit 4 [KO:K10755]
Dmel_CG5313  RfC3; replication factor C subunit 3 [KO:K10756]
Dmel_CG6258  RfC38; replication factor C 38kD subunit, isoform B [KO:K10756]
Dmel_CG13690  CG13690; uncharacterized protein [KO:K10743] [EC:3.1.26.4]
Dmel_CG11164  CG11164; uncharacterized protein, isoform B [KO:K10744]
Dmel_CG30105  CG30105; uncharacterized protein [KO:K10745]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   

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