KEGG   PATHWAY: dre00510Help
Entry
dre00510                    Pathway                                

Name
N-Glycan biosynthesis - Danio rerio (zebrafish)
Description
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
Class
Metabolism; Glycan biosynthesis and metabolism
BRITE hierarchy
Pathway map
dre00510  N-Glycan biosynthesis
dre00510

Ortholog table
Module
dre_M00055  N-glycan precursor biosynthesis [PATH:dre00510]
dre_M00072  N-glycosylation by oligosaccharyltransferase [PATH:dre00510]
dre_M00073  N-glycan precursor trimming [PATH:dre00510]
dre_M00075  N-glycan biosynthesis, complex type [PATH:dre00510]
Other DBs
BSID: 83559
GO: 0006487
Organism
Danio rerio (zebrafish) [GN:dre]
Gene
799960  dolk; dolichol kinase [KO:K00902] [EC:2.7.1.108]
566539  dpagt1; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
751635  alg5; dolichyl-phosphate beta-glucosyltransferase [KO:K00729] [EC:2.4.1.117]
436733  zgc:92907; uncharacterized protein LOC436733 [KO:K07432] [EC:2.4.1.141]
557181  alg13; UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]
450072  alg14; UDP-N-acetylglucosamine transferase subunit ALG14 homolog [KO:K07441] [EC:2.4.1.141]
445202  dpm1; dolichol-phosphate mannosyltransferase subunit 1 [KO:K00721] [EC:2.4.1.83]
796295  dpm2; dolichol phosphate-mannose biosynthesis regulatory protein [KO:K09658]
393782  dpm3; dolichol-phosphate mannosyltransferase subunit 3 [KO:K09659]
334161  alg1; chitobiosyldiphosphodolichol beta-mannosyltransferase [KO:K03842] [EC:2.4.1.142]
403068  alg2; alpha-1,3/1,6-mannosyltransferase ALG2 [KO:K03843] [EC:2.4.1.257 2.4.1.132]
407614  alg11; GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase isoform 1 [KO:K03844] [EC:2.4.1.131]
553725  alg3; dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [KO:K03845] [EC:2.4.1.258]
393598  alg9; alpha-1,2-mannosyltransferase ALG9 [KO:K03846] [EC:2.4.1.261 2.4.1.259]
569494  alg12; dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase precursor [KO:K03847] [EC:2.4.1.260]
445327  alg6; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase precursor [KO:K03848] [EC:2.4.1.267]
327601  alg8; probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03849] [EC:2.4.1.265]
100331575  alg10; asparagine-linked glycosylation 10 [KO:K03850] [EC:2.4.1.256]
266797  stt3a; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]
323918  stt3b; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
325561  rpn1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 precursor [KO:K12666]
335985  rpn2; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 precursor [KO:K12667]
566527  dad1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
553582  tusc3; tumor suppressor candidate 3 [KO:K12669]
406408  ddost; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor [KO:K12670]
474331  dolpp1; dolichyldiphosphatase 1 [KO:K07252] [EC:3.6.1.43]
567913  mogs; mannosyl-oligosaccharide glucosidase [KO:K01228] [EC:3.2.1.106]
100334730  ganab; neutral alpha-glucosidase AB [KO:K05546] [EC:3.2.1.207]
556237  man1b1b; endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase [KO:K01230] [EC:3.2.1.113]
562592  man1b1a; endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1 [KO:K01230] [EC:3.2.1.113]
566078  man1a1; mannosyl-oligosaccharide 1,2-alpha-mannosidase IA [KO:K01230] [EC:3.2.1.113]
335538  man1a2; mannosyl-oligosaccharide 1,2-alpha-mannosidase IB [KO:K01230] [EC:3.2.1.113]
101882461  mannosyl-oligosaccharide 1,2-alpha-mannosidase IA-like isoform X2 [KO:K01230] [EC:3.2.1.113]
393649  mgat1a; alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00726] [EC:2.4.1.101]
558524  mgat1b; mannoside acetylglucosaminyltransferase 1 [KO:K00726] [EC:2.4.1.101]
100333987  man2a2; alpha-mannosidase 2x isoform X1 [KO:K01231] [EC:3.2.1.114]
560219  man2a1; alpha-mannosidase 2 [KO:K01231] [EC:3.2.1.114]
100000478  mgat2; alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00736] [EC:2.4.1.143]
797512  fut8a; alpha-(1,6)-fucosyltransferase [KO:K00717] [EC:2.4.1.68]
768123  b4galt1l; beta-1,4-galactosyltransferase 1 [KO:K07966] [EC:2.4.1.- 2.4.1.38 2.4.1.90 2.4.1.22]
550425  b4galt1; beta-1,4-galactosyltransferase 1 [KO:K07966] [EC:2.4.1.- 2.4.1.38 2.4.1.90 2.4.1.22]
100148828  b4galt2; beta-1,4-galactosyltransferase 2 [KO:K07967] [EC:2.4.1.- 2.4.1.38 2.4.1.90 2.4.1.22]
556544  si:dkey-199f5.8; beta-1,4-galactosyltransferase 3 [KO:K07968] [EC:2.4.1.- 2.4.1.38 2.4.1.90]
561756  b4galt3; beta-1,4-galactosyltransferase 3 isoform X1 [KO:K07968] [EC:2.4.1.- 2.4.1.38 2.4.1.90]
445376  st6gal1; beta-galactoside alpha-2,6-sialyltransferase 1 [KO:K00778] [EC:2.4.99.1]
403116  st6gal2a; beta-galactoside alpha-2,6-sialyltransferase 2 [KO:K00779] [EC:2.4.99.1]
570828  mgat3b; beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
553676  mgat3a; beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
431727  mgat4b; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B [KO:K00738] [EC:2.4.1.145]
563038  mgat4a; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X1 [KO:K00738] [EC:2.4.1.145]
724006  mgat5; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A [KO:K00744] [EC:2.4.1.155]
101884072  mgat5b; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B [KO:K09661] [EC:2.4.1.-]
100333333  mgat4c; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C [KO:K13748] [EC:2.4.1.145 2.4.1.201]
492796  zgc:101663; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C [KO:K13748] [EC:2.4.1.145 2.4.1.201]
Compound
C00096  GDP-mannose
C00110  Dolichyl phosphate
C00381  Dolichol
C00621  Dolichyl diphosphate
C01246  Dolichyl beta-D-glucosyl phosphate
C03021  Protein asparagine
C03862  Dolichyl phosphate D-mannose
G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
G00002  N,N'-Chitobiosyldiphosphodolichol
G00003   
G00004   
G00005   
G00006   
G00007   
G00008   
G00009   
G00010   
G00011   
G00012   
G00013   
G00014   
G00015   
G00016   
G00017   
G00018  DS 3
G00019   
G00020   
G00021   
G00022   
G00171   
G10526   
G10595   
G10596   
G10597   
G10598   
G10599   
Reference
PMID:9111134
  Authors
Schutzbach JS.
  Title
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  Journal
Glycoconj J 14:175-82 (1997)
DOI:10.1023/A:1018533620002
Reference
  Authors
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  Title
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  Journal
Mol Biol Evol 19:1451-63 (2002)
Reference
  Authors
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  Title
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 278:22498-505 (2003)
DOI:10.1074/jbc.M302850200
Reference
  Authors
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  Title
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  Journal
J Biol Chem 276:21828-40 (2001)
DOI:10.1074/jbc.M010896200
Reference
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002
Reference
  Authors
O'Reilly MK, Zhang G, Imperiali B.
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry 45:9593-603 (2006)
DOI:10.1021/bi060878o
KO pathway
ko00510   

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