Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)

acetyl-CoA:hydrogencarbonate ligase (ADP-forming)

ATP + acetyl-CoA + hydrogencarbonate = ADP + phosphate + malonyl-CoA [RN:R00742]

R00742

ATP [CPD:C00002];
acetyl-CoA [CPD:C00024];
hydrogencarbonate [CPD:C00288]

This enzyme is a multi-domain polypeptide that catalyses three different activities - a biotin carboxyl-carrier protein (BCCP), a biotin carboxylase that catalyses the transfer of a carboxyl group from hydrogencarbonate to the biotin molecule carried by the carrier protein, and the transfer of the carboxyl group from biotin to acetyl-CoA, forming malonyl-CoA. In some organisms these activities are catalysed by separate enzymes (see EC 6.3.4.14, biotin carboxylase, and EC 2.1.3.15, acetyl-CoA carboxytransferase). The carboxylation of the carrier protein requires ATP, while the transfer of the carboxyl group to acetyl-CoA does not.

EC 6.4.1.2 created 1961, modified 2018