KEGG   ENZYME: 1.1.1.145
Entry
EC 1.1.1.145                Enzyme                                 
Name
3beta-hydroxy-Delta5-steroid dehydrogenase;
progesterone reductase;
Delta5-3beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene steroid dehydrogenase;
3beta-hydroxy steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid oxidoreductase;
3beta-hydroxy-5-ene-steroid oxidoreductase;
steroid-Delta5-3beta-ol dehydrogenase;
3beta-HSDH;
5-ene-3-beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene-steroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3beta-hydroxy-Delta5-steroid:NAD+ 3-oxidoreductase
Reaction(IUBMB)
a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH + H+ [RN:R03176]
Reaction(KEGG)
Substrate
3beta-hydroxy-Delta5-steroid [CPD:C03836];
NAD+ [CPD:C00003]
Product
3-oxo-Delta5-steroid [CPD:C01034];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced cosubstrate, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
History
EC 1.1.1.145 created 1972
Pathway
ec00140  Steroid hormone biosynthesis
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00070  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K16045  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K26407  Poxviridae/Iridoviridae 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Genes
HSA3283(HSD3B1) 3284(HSD3B2)
PTR457165(HSD3B2) 469446
PPS100982464(HSD3B2) 100983009 100990187
GGO101141070 101141801 101146563(HSD3B2)
PON100451655 100452394 100455460
PPYG129015890 129015909 129015918
NLE100596087 100605995(HSD3B2) 100606563 100607955
HMH116469522 116469530(HSD3B2)
SSYN134733865 134733875 134734675(HSD3B1)
MCC712686(HSD3B2) 713091 713272 715337
MCF102133592 102134747 102135110
MTHB126931035 126931104 126931154 126951788
MNI105479071 105479074 105479078 105479082 105479084(HSD3B2)
CSAB103224104 103224109(HSD3B2)
CATY105599590 105599597(HSD3B2)
PANU100999145 101018740 101019096(HSD3B2)
TGE112624233 112634262(HSD3B2)
MLEU105541296(HSD3B2) 105541330
RRO104654599 104654602 104654605
RBB108528160 108528161 108528164
TFN117074250 117074256
PTEH111549589 111549591
CANG105505264 105505267
CJC100387484 100389654 100400416 100405879
SBQ101042680 101051416
CIMI108297130 108301104
ANAN105721680(HSD3B2) 105721706 110567017
CSYR103258418(HSD3B2)
MMUR105869786(HSD3B2)
LCAT123635118(HSD3B2)
PCOQ105809629(HSD3B2)
OGA100966795
MMU100043456(Hsd3b8) 100043461(Hsd3b9) 15492(Hsd3b1) 15493(Hsd3b2) 15494(Hsd3b3) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
MCAL110288898 110290813 110290978 110291292 110291371 110291888
MPAH110315378 110319834 110319836 110320478 110320490
RNO24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
MCOC116093252 116094199 116094200 116094201
ANU117707219 117707246 117707247 117707273 117707523
MUN110542496 110543775 110544885 110544899 110563733 110563735 132656956 132656957 132656958 132656961 132656962 132656967 132657118
CGE100770922 113830678 113833179 113833198
MAUA101837099 101842851(Hao2) 121138738 121142382
PROB127232674 127232675
PLEU114688199 114688619 114688620 114688621 114688750 114688751 114688752 114698547
MORG121437803 121437911 121438015 121438017
MFOT126490105 126510847
AAMP119801286 119801287 119801288 119801289
NGI103728305 103732007 103738837
HGL101707488(Hsd3b1) 101707858
CPOC100379573(Hsd3b1) 100725531 100725801
CCAN109702728 109702729
DORD105991916 105991917
DSP122118370 122118374
PLOP125344738 125345248 125345249 125345254 125345257 125345259 125345261 125355478 125355479 125355483
NCAR124964015 124990189 124992819
MMMA107156205(Hao2) 107156207 107156209
OCU100346986 100347914
OPI101522373 101522782
TUP102470981
GVR103584548 103607308
CFA483146(HSD3B2)
CLUD112664108
VVP112916789
VLG121490669
NPO129501295
AML100464280(HSD3B1)
UMR103677123
UAH113244362
UAR123795008
ELK111143436
LLV125099255
MPUF101680243
MNP132000700
MLK131809998
NVS122898812
ORO101378904
EJU114208061(HSD3B2)
ZCA113909475(HSD3B2)
MLX118000525(HSD3B2)
NSU110580487(HSD3B2)
LWW102742748
FCA100616070(HSD3B2)
PYU121027796
PCOO112872261
PBG122481024
PVIV125171536
LRUF124514119
PTG102957262
PPAD109272160
PUC125912196
AJU106978496
HHV120247640
BTA281824(HSD3B1)
BOM102274088
BIU109555859
BBUB102413429
BBIS104993819
CHX100860869(3BHSD)
OAS100190978(HSD3B1)
BTAX128045498
ODA120855999
CCAD122435367
MBEZ129541051
SSC445539(HSD3B1)
CFR102512447
CBAI105079257
CDK105089475
VPC102528409
BACU103015346
BMUS118884274
LVE103083046
OOR101284283
DLE111179902
PCAD102987751
PSIU116758472
NASI112413050
ECB100034078(HSD3B2)
EPZ103548379
EAI106832920(HSD3B2)
MYB106723550(HSD3B2)
MMYO118672875(HSD3B2)
MLF106694328(HSD3B2)
PKL118727130
EFUS103291359(HSD3B2)
MNA107545288(HSD3B2)
DRO112314004(HSD3B2)
SHON118981952(HSD3B2)
AJM119041699(HSD3B2)
PDIC114489256
PHAS123804497(HSD3B2)
MMF118637661
PPAM129063973
HAI109389868(HSD3B2)
RFQ117014453(HAO2)
PALE102891879
PGIG120585109
PVP105296358
RAY107518830(HSD3B2)
MJV108390800
TOD119236234
SARA101555226 101555490
SETR125997493
LAV100659049(HSD3B2)
TMU101358325
ETF101663671(HSD3B1)
DNM101447815
MDO100012668
GAS123247232
SHR100913530(HSD3B2)
AFZ127559197
PCW110219672(HSD3B2)
TVP118856490(HSD3B2)
OAA100080135
GGA396015(HSD3B1)
PCOC116244326
MGP104915630
CJO107319373
TPAI128076148 128090414
LMUT125684639(HSD3B1)
NMEL110398845
APLA101803865(HSD3B1)
ACYG106045741(HSD3B1)
CATA118259816(HSD3B1)
AFUL116502173
TGU751770(HSD3B1)
LSR110480744
SCAN103827136
PMOA120506495
OTC121339296
PRUF121351666
GFR102039409
FAB101817697
OMA130265090
PHI102101526
PMAJ107211459
CCAE111938581
CCW104687106
CBRC103613933
ETL114058198
ZAB102062520
ZLE135452668
ACHL103801073
SVG106857875
MMEA130582277
HRT120749079
FPG101921564
FCH102052697
CLV102087460
EGZ104126518
NNI104008615
PCRI104035039
PLET104617853
EHS104512760
PCAO104053168
ACUN113485872
TALA104357901
PADL103921223
AFOR103905431
ACHC115344139
HALD104319887
HLE104831735
AGEN126049087
GCL127029573
CCRI104162430
CSTI104558363
CMAC104476511
MUI104539044
BREG104641481
FGA104079993
GSTE104255879
LDI104351289
MNB103779544
OHA104327359
NNT104410259
SHAB115601668
DPUB104298412
PGUU104468993
ACAR104520429
CPEA104384975
AVIT104277939
CVF104293163
RTD128904122
CUCA104055667
TEO104373662
BRHI104496619
AAM106497227
AROW112963963
NPD112960083
TGT104576502
DNE112985919
SCAM104150689
ASN102367786(HSD3B1)
AMJ109280168(HSD3B1)
CPOO109319128(HSD3B1)
GGN109304596(HSD3B1)
PSS102453952
CMY102934871
CCAY125622274
DCC119843575
CPIC101946936
TST117886232
CABI116817817
MRV120396043
ACS100561899(hsd3b1)
ASAO132766487(HSD3B1)
PVT110076926
SUND121922906
PBI103067418
PMUR107285816
CTIG120306125
TSR106542700
PGUT117661842
APRI131193284(HSD3B1)
PTEX113444507
NSS113417241
VKO123033249
PMUA114587929(HSD3B2)
PRAF128404595(HSD3B2)
ZVI118075222(HSD3B2)
HCG128345684
GJA107114856
STOW125425234(HSD3B2)
EMC129345698
XLA734818(hsd3b1.L)
XTR100490622
NPR108802532
RTEM120928726
BBUF120994332
MUO115471398(HSD3B1)
GSH117358938(HSD3B1)
DRE373131(hsd3b2) 553532(hsd3b1)
SRX107712267
SANH107658243
SGH107569293
CCAR109052439
CAUA113108554 113108799
CGIB127965123 128019700
PTET122351771(hsd3b1)
LROH127170525(hsd3b1)
OMC131546787(hsd3b1)
PPRM120466714(hsd3b1)
RKG130076948(hsd3b1)
MAMB125270537(hsd3b1)
CIDE127518655
MASI127448584
TROS130555852(hsd3b1)
TDW130427658(hsd3b1)
MANU129451507(hsd3b1)
IPU100304475(hsd3b1)
IFU128609137(hsd3b1)
PHYP113524942(hsd3b1)
SMEO124379336(hsd3b1)
TFD113654422(hsd3b1)
TVC132850628(hsd3b1)
TRN134323829(hsd3b1)
AMEX103027852(hsd3b1)
CMAO118800102(hsd3b1)
EEE113587753
CHAR105913236 105913280 105913281
TRU101069884(hsd3b1)
TFS130530194(hsd3b1)
TNGGSTEN00029150G001
LCO104921488(hsd3b1)
TBEN117495881(hsd3b1)
CGOB115026845(hsd3b1)
PGEO117466261(hsd3b1)
GACU117536557(hsd3b1)
EMAC134867270(hsd3b1)
ELY117251590(hsd3b1)
EFO125900207(hsd3b1)
PLEP121949401(hsd3b1) 121963657
SLUC116059361(hsd3b1)
ECRA117953315(hsd3b1)
ESP116697664
PFLV114564020
GAT120833929(hsd3b1)
PPUG119228951(hsd3b1)
AFB129104536(hsd3b1)
CLUM117750405(hsd3b1)
PSWI130204482(hsd3b1)
MSAM119884914 119904488(hsd3b1)
SCHU122885279(hsd3b1)
CUD121523133(hsd3b1)
ALAT119025245(hsd3b1)
MZE101485719
ONL100534568(hsd3b1)
OAU116332553(hsd3b1)
OLA100144358(3bhsd)
OML112138010(hsd3b1)
CSAI133446387(hsd3b1)
XMA102228303
XCO114147930
XHE116723048
PRET103477979
PFOR103152152
PLAI106939754
PMEI106917521
GAF122839771(hsd3b1)
PPRL129360909(hsd3b1)
CVG107087352
CTUL119774713(hsd3b1)
GMU124871021(hsd3b1)
NFU107389612(hsd3b1)
KMR108244192(hsd3b1)
ALIM106531920
NWH119419363(hsd3b1)
AOCE111570570(hsd3b1)
MCEP125017257(hsd3b1)
CSEM103391994(hsd3b1)
POV109637945
SSEN122785783(hsd3b1)
HHIP117755029 117755030
HSP118119640 118120310
PPLT128455946(hsd3b1)
SMAU118283187(hsd3b1)
LCF108902437
SDU111218347
SLAL111652102
XGL120801969(hsd3b1)
HCQ109522071
SSCV125973254
SBIA133511988(hsd3b1)
PEE133410274(hsd3b1)
PTAO133486409(hsd3b1)
BPEC110156192(hsd3b1)
MALB109951646
BSPL114847637(hsd3b1)
SJO128367682(hsd3b1)
SASA106586600
STRU115161098
OTW112240651(hsd3b1)
OMY100136743(hsd3b1)
OGO124044100(hsd3b1)
ONE115112196
OKI109869285
OKE118371546(hsd3b1)
SALP111978723
SNH120058421(hsd3b1)
CCLU121536290(hsd3b1)
ELS105016430
SFM108937872
PKI111860692
AANG118214002 118214003
LOC102685561(hsd3b1)
PSPA121320746(hsd3b1)
ARUT117406697 117406856
PSEX120522925(hsd3b1)
LCM102345588
CMK103180179(hsd3b1) 121852133
RTP109918392(hsd3b1)
HOC132820585(hsd3b1)
LERI129702520(hsd3b1)
PMRN116954501
LRJ133347820
BFO118415735 118426116
BBEL109470289 109481615
LPIC129254859
DMEDmel_CG7724(CG7724)
DER6543694
DSIDsimw501_GD14693(Dsim_GD14693)
DYADyak_GE19901
DSR110187899 110190103
DPO4813984
DPE6603899
DMN108165559
DWI6645028
DGR6558385
DMODmoj_GI12485
DAZ108612818
DNV108651018
DHE111602930
CCAT101455915
BOD106619208
BDR105225278
RZE108370312
AOQ129240938
TDA119666775
MDE101899660
SCAC106092781
LCQ111691019
LSQ119600382
GFS119645278
ECOE129948506
CLON129611542
HIS119650207
AGA1277239
ACOZ120951979
AARA120897593
AMER121593679
ASTE118514455
AFUN125767669
AMOU128302512
AALI118465094
AAG5566270
AALB109407756 109418935
ASUA134214407
CQUCpipJ_CPIJ003056
CPII120432614
CNS116347823
BCOO119081235
NVI100120136
CSOL105366624
TPRE106650065
FAS105264423
DAM107039335
VCAN122411680
CCIN107268819
NPT124218962
NFB124182898
NLO107222152
NVG124305414
AROA105683351
DCI103516518
CLEC106661338
NLU111044911
HVI124367113
MQU128995074
FOC113210108
TPAL117644725
ZNE110831195
CSEC111870363
IEL124167557
FCD110862702
DMK116926030
DPZ124315410
PVM113815316
PJA122252315
PCHN125025755
PMOO119576789
HAME121875688
PCLA123764960
CQD128692025
PTRU123519041
ESN126998836
MNZ135202439
ISC8053025
DSV119445622
RSAN119377584 119390488
RMP119169799
VDE111247588 111254724
VJA111264476 111268118
TUT107363285
DPTE113794090
DFR124490209
CSCU111622487
PTEP107437550
ABRU129960448
UDV129219275
SDM118201694
LPOL106475760
PMEO129587684
HROHELRODRAFT_89375
PVUL126824716
GAE121389292
HRF124140226 124141700
HRJ124260823 124278058
CRG105321517 105332656
CANU128186867 128188117 128188594
CVN111136775 111137023 111137166
OED125654276 125659193 125659727
MYI110457155
PMAX117333059 117333060 117333062 117333069
MMER123525098 123534278 123534660 123535125 123535257 123535266 123552007
RPHI132742228 132742229 132743368 132753356 132759419 132759420
DPOL127839711 127856352 127861538
MAEA128229618 128232487 128232575
OSN115219210
LJP135468058 135477165
LAK106151343
LLON135485103 135502377
ENWMJO57_19210
EMPEZMO1_1200(nsdHL)
PWUA8O14_06055
MTURv1106c
MTVRVBD_1106c
MTCMT1137
MRAMRA_1117
MTFTBFG_11128
MTBTBMG_02877
MTKTBSG_02893
MTZTBXG_002857
MTGMRGA327_06925
MTIMRGA423_06915
MTECCDC5079_1022
MTURCFBS_1174
MTLCCDC5180_1014
MTOMTCTRI2_1136
MTDUDA_1106c
MTNERDMAN_1239
MTJJ112_05975
MTUBMT7199_1133
MTUCJ113_07785
MTUEJ114_05980
MTXM943_05820
MTUHI917_07855
MTULTBHG_01091
MTUTHKBT1_1172
MTUUHKBT2_1178
MTQHKBS1_1174
MBOBQ2027_MB1136C
MBBBCG_1166c
MBTJTY_1139
MBMBCGMEX_1138c
MBKK60_011910
MBXBCGT_0934
MAFMAF_11210
MMICRN08_1242
MCEMCAN_11161
MCQBN44_11231
MCVBN43_30163
MCXBN42_20954
MCZBN45_30150
MORYMO_001188
MLEML1942
MLBMLBr01942
MPAMAP_2688
MAOMAP4_1128
MAVIRC58_05605
MAVURE97_05600
MAVMAV_1225
MITOCO_11340
MIAOCU_11320
MIDMIP_01824
MYOOEM_11450
MCHIAN480_06750
MIROCQ_11350
MMALCKJ54_05520
MLPMLM_1173
MMANMMAN_54580
MSAMycsm_05045
MULMUL_0172
MMIMMAR_4359
MMAEMMARE11_41790
MLIMULP_04570
MPSEMPSD_44990
MSHOMSHO_16920
MMCMmcs_4109
MKMMkms_4185
MJLMjls_4340
MMMW7S_05545
MKNMKAN_07985
MYVG155_22700
MYEAB431_06460
MHADB586_07090
MDXBTO20_08365
MSHGMSG_03923
MFJMFLOJ_47450
MSTOMSTO_51430
MSIMMSIM_37390
MSAKMSAS_22240
MKUI2456_20620
MLWMJO58_21440
MXEMYXE_14010
MNVMNVI_06140
MPAGC0J29_23965
MNMMNVM_07790
MGORH0P51_22240
MCOOMCOO_20360
MBAIMB901379_03818
MSEOMSEO_27010
MHEKJMUB5695_01344
MGAUMGALJ_31210
MLJMLAC_06830
MBRDMBRA_27460
MSHJMSHI_03080
MMAMK3U93_19010
MSPGF6B93_17425
MOTLTS72_03400
MLMMLPF_2463(hsd)
MPAAMKK62_05735
MKYIWGMT90018_50180
MADIA7U43_09340
MWUPT015_11765
MSMMSMEG_5228
MSGMSMEI_5091
MSBLJ00_25860
MSNLI99_25865
MSHLI98_25870
MVAMvan_4635
MGIMflv_2075
MSPMspyr1_14990
MCBMycch_4050
MNED174_21420
MYNMyAD_21005
MFTXA26_46570
MPHLMPHLCCUG_04229
MVQMYVA_4444
MLLB1R94_06430
MRHMycrhN_3550
MTHN4412656_03537
MHASMHAS_03213
MDUMDUV_00690
MCHTMCHIJ_25440
MAUUNCTC10437_04362
MMAGMMAD_42700
MMORMMOR_18320
MFXMFAL_37920
MAICMAIC_43720
MIJMINS_38420
MALVMALV_48580
MTYMTOK_04970
MPSCMPSYJ_22130
MARZMARA_54320
MGADMGAD_52480
MHEVMHEL_09990
MSARMSAR_27010
MANYMANY_26880
MAUBMAUB_43590
MPOFMPOR_47010
MPHUMPHO_25810
MMUCC1S78_005420
MMATMMAGJ_05590
MBOKMBOE_27320
MFGK6L26_09070
MSEIMSEDJ_54960
MFLVNCTC10271_01108
MCEEMCEL_44150
MMONEWR22_22255
MGOAFA91_14490
MHOLK3U96_06035
MSENK3U95_21790
MPAEK0O64_06045
MRFMJO55_20980
MDFK0O62_22395
MCROMI149_06755
MGROFZ046_26400
MKRMKOR_40410
MPAKMIU77_04215
MJDJDM601_1056
MTER4434518_01040
MMINMMIN_27080
MHIBMHIB_06150
MHERK3U94_05970
MVMMJO54_06060
SNFJYK04_07456(auaH_3)
LOBNEF87_000807
 » show all
Reference
1  [PMID:4226148]
  Authors
Cheatum SG, Watten JC.
  Title
Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.
  Journal
Biochim Biophys Acta 122:1-13 (1966)
DOI:10.1016/0926-6593(66)90086-5
Reference
2  [PMID:14220672]
  Authors
KORITZ SB.
  Title
THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY SMALL PARTICLES FROM RAT ADRENAL.
  Journal
Biochemistry 3:1098-102 (1964)
DOI:10.1021/bi00896a015
Reference
3
  Authors
Neville, A.M., Orr, J.C. and Engel, L.L.
  Title
Delta5-3beta-Hydroxy steroid dehydrogenase activities of bovine adrenal cortex.
  Journal
Biochem J 107:20 (1968)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.145
IUBMB Enzyme Nomenclature: 1.1.1.145
ExPASy - ENZYME nomenclature database: 1.1.1.145
BRENDA, the Enzyme Database: 1.1.1.145
CAS: 9044-85-3

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