KEGG ENZYME: 1.1.1.170

ENZYME: 1.1.1.170

Entry

EC 1.1.1.170 Enzyme

Name

3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating);
3beta-hydroxy-4beta-methylcholestenecarboxylate 3-dehydrogenase (decarboxylating);
3beta-hydroxy-4beta-methylcholestenoate dehydrogenase;
sterol 4alpha-carboxylic decarboxylase;
sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating) (ambiguous);
ERG26 (gene name);
NSDHL (gene name)

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

3beta-hydroxysteroid-4alpha-carboxylate:NAD(P)+ 3-oxidoreductase (decarboxylating)

Reaction(IUBMB)

a 3beta-hydroxysteroid-4alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H [RN:R04483 R07149]

Reaction(KEGG)

R04483 R07149;
(other) R04328 R07494

Substrate

3beta-hydroxysteroid-4alpha-carboxylate;
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

3-oxosteroid [CPD:C01876];
CO2 [CPD:C00011];
NADH [CPD:C00004];
NADPH [CPD:C00005]

Comment

The enzyme catalyses the decarboxylation of the C-4 carbon and the dehydrogenation of a 3beta hydroxyl at the C-3 carbon of 3beta-hydroxysteroid-4alpha-carboxylates. It is involved in zymosterol and cholesterol biosynthesis.

History

EC 1.1.1.170 created 1978, modified 2002, modified 2012

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

ec01130

Biosynthesis of antibiotics

Orthology

K07748

sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating)

Genes

HSA:	50814(NSDHL)

PTR:	465960(NSDHL)

PPS:	100975917(NSDHL)

GGO:	101144867(NSDHL)

PON:	100172372(NSDHL)

NLE:	100596441(NSDHL)

MCC:	693333(NSDHL)

MCF:	102137627(NSDHL)

CSAB:

103232767(NSDHL)

RRO:	104654022(NSDHL)

RBB:	108513868(NSDHL)

CJC:	100398582(NSDHL)

SBQ:	101027738(NSDHL)

MMU:	18194(Nsdhl)

MCAL:

110287212(Nsdhl)

MPAH:

110313833(Nsdhl)

RNO:	309262(Nsdhl)

MUN:	110540271(Nsdhl)

CGE:	100764429(Nsdhl) 100768776

NGI:	103731179(Nsdhl)

HGL:	101716507(Nsdhl)

CCAN:

109695438(Nsdhl)

OCU:	100351296(NSDHL)

TUP:	102500582(NSDHL)

CFA:	481079(NSDHL)

VVP:	112912365(NSDHL)

AML:	100466780(NSDHL)

UMR:	103674163(NSDHL)

UAH:	113246811(NSDHL)

ORO:	101372464(NSDHL)

FCA:	101096551(NSDHL)

PTG:	102959228(NSDHL)

AJU:	106971243(NSDHL)

BTA:	616694(NSDHL)

BOM:	102276234(NSDHL)

BIU:	109555394(NSDHL)

BBUB:

102398147(NSDHL)

PHD:	102328837(NSDHL)

CHX:	102173170(NSDHL)

OAS:	101112792(NSDHL)

SSC:	100157894(NSDHL)

CFR:	102516550(NSDHL)

CDK:	105104051(NSDHL)

BACU:

103020703(NSDHL)

LVE:	103087463(NSDHL)

OOR:	101278134(NSDHL)

DLE:	111170601(NSDHL)

ECB:	100063648(NSDHL)

EPZ:	103543383(NSDHL)

EAI:	106834275(NSDHL)

MYB:	102257708(NSDHL)

MYD:	102771848(NSDHL)

MNA:	107537970(NSDHL)

HAI:	109394344(NSDHL)

RSS:	109434385(NSDHL) 109441062

DRO:	112296338(NSDHL)

PALE:

102879960(NSDHL)

LAV:	100671133(NSDHL)

TMU:

101357526

MDO:	100026414(NSDHL)

SHR:	100914940(NSDHL)

PCW:	110199978(NSDHL)

OAA:	103171093(NSDHL)

GGA:	422302(NSDHL)

MGP:	100544971(NSDHL)

CJO:	107312870(NSDHL)

APLA:

101790127(NSDHL)

ACYG:

106047509(NSDHL)

TGU:	100190543(NSDHL)

LSR:	110477979(NSDHL)

SCAN:

103816322(NSDHL)

GFR:	102041531(NSDHL)

FAB:	101814664(NSDHL)

PHI:	102100867(NSDHL)

PMAJ:

107204042(NSDHL)

CCAE:

111929260(NSDHL)

CCW:	104691595(NSDHL)

ETL:	114062107(NSDHL)

FPG:	101921503(NSDHL)

FCH:	102048326(NSDHL)

CLV:	102098029(NSDHL)

EGZ:	104127975(NSDHL)

NNI:	104021094(NSDHL)

ACUN:

113480109(NSDHL)

AAM:	106489838(NSDHL)

ASN:	102383140(NSDHL)

AMJ:	102562140(NSDHL)

PSS:	102447619(NSDHL)

CMY:	102945577(NSDHL)

CPIC:

101937255(NSDHL)

ACS:	100562829(nsdhl)

PVT:	110088403(NSDHL)

PBI:	103061373(NSDHL)

PMUR:

107293162(NSDHL)

GJA:	107118212(NSDHL)

XLA:	108700163(nsdhl.S) 496236(nsdhl.L)

XTR:	550044(nsdhl)

NPR:	108795343(NSDHL)

DRE:	550369(nsdhl)

SRX:	107733445(nsdhl)

SANH:

107663101(nsdhl)

SGH:	107554660(nsdhl)

CCAR:

109059120 109102549

IPU:	108268902(nsdhl)

PHYP:

113542904(nsdhl)

AMEX:

103027604(nsdhl)

EEE:	113576547(nsdhl)

TRU:	101061359(nsdhl)

TNG:	GSTEN00017071G001

LCO:	104923201(nsdhl)

NCC:	104948435(nsdhl)

MZE:	101480912(nsdhl)

OLA:	101171010(nsdhl)

XMA:	102221009(nsdhl)

XCO:	114139190(nsdhl)

PRET:

103471168(nsdhl)

NFU:	107395696(nsdhl)

KMR:	108242032(nsdhl)

CSEM:

103391149(nsdhl)

LCF:	108882885(nsdhl)

SDU:	111226496(nsdhl)

HCQ:	109515829(nsdhl)

BPEC:

110163595(nsdhl)

MALB:

109968402(nsdhl)

SASA:

106604248 106612199

OTW:	112221226 112247746

SALP:

112069177 112073419

ELS:	105025751(nsdhl)

SFM:	108925361(nsdhl)

PKI:	111843704(nsdhl)

LCM:	102355835(NSDHL)

CMK:	103177331(nsdhl)

BFO:	BRAFLDRAFT_117175

SPU:

577670

APLC:

110983741

SKO:

100367277

HRO:	HELRODRAFT_185437

LGI:	LOTGIDRAFT_125131

PCAN:

112560719

LAK:

106150518

TAD:	TRIADDRAFT_56172

AQU:	100641976 105314342

ATH:	AT1G47290(3BETAHSD/D1) AT2G26260(3BETAHSD/D2) AT2G43420

ALY:	ARALYDRAFT_473882(AT3BETAHSD/D1) ARALYDRAFT_481483(AT3BETAHSD/D2) ARALYDRAFT_483452

CRB:	17888910 17889700 17898596

CSAT:

104703154 104714089 104752257 104758028 104777665 104782858 104785013 104793273 109124732

EUS:	EUTSA_v10001381mg EUTSA_v10001949mg EUTSA_v10011495mg

BRP:	103842157 103843901 103866715

BNA:	106368540 106371310 106390851 106400657 106402151 106414952 106421095

BOE:	106296378 106307642 106308495 106334255 106341839

THJ:	104802316 104803571 104804450 104819910 104819911

CPAP:

110812016 110821405

CIT:	102607843 102626005

CIC:	CICLE_v10007884mg CICLE_v10019433mg

TCC:	18588175 18602449

GRA:	105766177 105773475 105778202 105796377

GHI:	107896916 107901017 107903885 107914644 107927476 107938719 107957226 107961633

GAB:	108476304 108483574 108488634

DZI:	111279431 111285384 111303015 111307734

EGR:	104435786 104436360

GMX:	100794808 100797101 100799363 100800800 100809319 100814147 100815785 100816854 102666674 102667763

PVU:	PHAVU_009G084200g PHAVU_011G053200g

VRA:	106756699 106760856 106776089

VAR:	108330047 108331511 108340625

CCAJ:

109791368 109801541

MTR:	MTR_3g105440 MTR_4g068110

CAM:	101494810 101500996

LJA:	Lj0g3v0192789.1(Lj0g3v0192789.1) Lj2g3v0856600.1(Lj2g3v0856600.1) Lj3g3v3325980.1(Lj3g3v3325980.1)

ADU:	107470664 107470953 107476756

AIP:	107620898 107623291 107628972

LANG:

109325732 109328420 109341751 109343798

FVE:

PPER:

PMUM:

PAVI:

MDM:	103415386 103419184 103440612 103455762

PXB:	103933227 103949660 103953014

ZJU:	107405093 107420046 107422105

CSV:	101205275 101212494

CMO:	103483967 103488530

MCHA:

CMAX:

CMOS:

CPEP:

RCU:	8258610 8287636

JCU:	105628414 105638060

HBR:	110639226 110662259

MESC:

110604012 110613045

POP:	18100844 7492281

JRE:	108985075 108990435

QSU:	111984500 111989054 111989557 111999340 112002495 112007898 112015612 112021913 112023362 112026924 112029405

VVI:	100245662 100257631

SLY:	101249150 101254144 101256443

SPEN:

107005180 107011682 107011815

SOT:	102587792 102596460 102598251

CANN:

107857986 107859693 107863753

NTA:	107780439 107795088 107795373 107814161 107817215 107818175

NSY:	104216879 104239655 104245967

NTO:	104084496 104092502 104108740

NAU:	109210739 109213017 109227010

INI:	109161741 109184677

SIND:

105164848 105165847 105172010

OEU:	111371187 111377242 111377243 111380101 111393178 111393378

HAN:	110899855 110908422 110917497 110920605

LSV:	111875990 111883224 111899990 111919546

CCAV:

112503045 112508871 112511529 112522090

DCR:	108197059 108197988 108203699 108217750 108223476

BVG:	104888851 104904629

SOE:	110787809 110802274

CQI:	110682749 110696539 110717644 110738779

NNU:	104588912 104605277

PSOM:

113297813 113300464 113301315 113328380 113348744

OSA:	4333070 4333071 4347580

DOSA:

Os03t0404800-01(Os03g0404800) Os03t0405000-01(Os03g0405000) Os09t0516500-00(Os09g0516500)

OBR:	102699988 102711055 102711335

BDI:	100832384 100838723 100841751

ATS:	109751859(LOC109751859) 109759047(LOC109759047) 109759249(LOC109759249) 109776475(LOC109776475) 109780969(LOC109780969) 109782739(LOC109782739)

SBI:	110431777 110431804 8063889 8077068 8077069 8077070 8077071 8077107 8077112

ZMA:	100283692 100383338 103647586 103648872

SITA:

101762396 101772970 101776881 101778928 101779594 101779992

PDA:	103702195 103706039

EGU:	105035203 105056704

MUS:	103968507 103992669

DCT:	110109494 110113291

PEQ:	110038378 110039408

AOF:	109829223 109838803 109844846

ATR:	18431743 18433647 18448731

SMO:	SELMODRAFT_129445 SELMODRAFT_84229

PPP:

112282407

CRE:	CHLREDRAFT_58501(BHSD1)

VCN:	VOLCADRAFT_79975

MNG:

MNEG_5826

BPG:	Bathy08g03720

MIS:	MICPUN_62297

CSL:	COCSUDRAFT_54657

CVR:	CHLNCDRAFT_49861

APRO:

F751_0534

CME:	CYME_CMP217C

GSL:	Gasu_22080

CCP:	CHC_T00003646001

SCE:	YGL001C(ERG26)

AGO:	AGOS_AFR001W

ERC:

Ecym_6178

KLA:	KLLA0_C18513g

KMX:	KLMA_40026(ERG26)

LTH:	KLTH0E08338g

VPO:	Kpol_1025p36

ZRO:	ZYRO0G10582g

CGR:	CAGL0G00594g

NCS:	NCAS_0A05210(NCAS0A05210)

NDI:	NDAI_0K02600(NDAI0K02600)

TPF:	TPHA_0K01550(TPHA0K01550)

TBL:	TBLA_0E01630(TBLA0E01630)

TDL:	TDEL_0F02190(TDEL0F02190)

KAF:	KAFR_0F03320(KAFR0F03320)

PPA:	PAS_chr1-4_0604

DHA:	DEHA2C11550g

PIC:	PICST_71224(ERG26)

PGU:	PGUG_05873

SPAA:

SPAPADRAFT_58811

LEL:	LELG_04583

CAL:	CAALFM_C406270CA(ERG26)

CTP:	CTRG_04041

COT:	CORT_0E04240

CDU:	CD36_45790(ERG26)

CTEN:

CANTEDRAFT_114564

YLI:	YALI0C22165g

CLU:	CLUG_01042

CLUS:

A9F13_09g00891

CAUR:

CJI97_000938

SLB:	AWJ20_2805(ERG26)

NCR:

NCU02693

NTE:	NEUTE1DRAFT119017(NEUTE1DRAFT_119017)

SMP:	SMAC_04350

PAN:	PODANSg2055

TTT:	THITE_129164

MTM:	MYCTH_122939

CTHR:

CTHT_0009530

MGR:	MGG_04938 MGG_11399

TMN:	UCRPA7_2436 UCRPA7_5729

SSCK:

SPSK_09448 SPSK_09457

FGR:	FGSG_01203 FGSG_11714

FPU:	FPSE_01810

FVR:	FVEG_01330 FVEG_12076 FVEG_14610

FOX:	FOXG_00186 FOXG_03780 FOXG_18054

NHE:	NECHADRAFT_88124 NECHADRAFT_98704

TRE:	TRIREDRAFT_78929

MAW:	MAC_04166 MAC_06812

MAJ:	MAA_01307 MAA_07206

CMT:

CCM_07037

PLJ:	VFPFJ_03411

VAL:	VDBG_04735

VDA:	VDAG_04277

CFJ:	CFIO01_02970 CFIO01_11980

ELA:	UCREL1_10141 UCREL1_11513

PFY:	PFICI_00577 PFICI_10785

SSL:	SS1G_00993 SS1G_06585 SS1G_08084 SS1G_09117

BFU:	BCIN_15g00310

MBE:

MBM_00113

PSCO:

LY89DRAFT_460429 LY89DRAFT_644372 LY89DRAFT_687795 LY89DRAFT_690035 LY89DRAFT_697537 LY89DRAFT_786714

GLZ:	GLAREA_03076 GLAREA_04261 GLAREA_05189 GLAREA_06506 GLAREA_07025 GLAREA_07964 GLAREA_09332 GLAREA_09630 GLAREA_10161 GLAREA_11673 GLAREA_11772

ANI:	AN7575.2 AN8440.2

AFM:	AFUA_2G15030 AFUA_2G17400

AOR:	AO090120000187

ANG:	ANI_1_470134(An15g03090)

AFV:	AFLA_016870 AFLA_088000 AFLA_091760

ACT:	ACLA_072440 ACLA_081520

NFI:	NFIA_090280 NFIA_098940

PCS:	Pc12g00300 Pc18g05820

PDP:	PDIP_04860 PDIP_70700

CIM:	CIMG_02365

CPW:	CPC735_040060

PBL:	PAAG_11343(PAAG_01699)

PBN:	PADG_00078

URE:	UREG_01388

ABE:	ARB_03639 ARB_07982

TVE:	TRV_01960 TRV_04269

AJE:	HCAG_00257 HCAG_05272

PNO:	SNOG_03352 SNOG_13918 SNOG_15264

PTE:

PTT_14047

BZE:	COCCADRAFT_38332 COCCADRAFT_89445 COCCADRAFT_91862

BSC:	COCSADRAFT_145696 COCSADRAFT_162726 COCSADRAFT_38364

BOR:	COCMIDRAFT_103766 COCMIDRAFT_3390 COCMIDRAFT_9629

AALT:

CC77DRAFT_1044889 CC77DRAFT_590852 CC77DRAFT_933025

ZTR:	MYCGRDRAFT_32274(ERG26) MYCGRDRAFT_45223 MYCGRDRAFT_67122

PFJ:	MYCFIDRAFT_27681 MYCFIDRAFT_31190

BCOM:

BAUCODRAFT_323543 BAUCODRAFT_36849

NPA:	UCRNP2_2416

TML:	GSTUM_00005894001

SPO:	SPBC3F6.02c

CNE:

CNJ00460

CNB:

CNBJ3060

CGI:	CGB_D9350C

TMS:	TREMEDRAFT_67694

PPL:	POSPLDRAFT_90796 POSPLDRAFT_94333

TVS:	TRAVEDRAFT_122801

DSQ:	DICSQDRAFT_56128

PCO:	PHACADRAFT_265060

SHS:	STEHIDRAFT_125133 STEHIDRAFT_171904 STEHIDRAFT_66049

HIR:	HETIRDRAFT_475223

PSQ:	PUNSTDRAFT_143387 PUNSTDRAFT_82149

ADL:	AURDEDRAFT_184737

FME:	FOMMEDRAFT_113387

GTR:	GLOTRDRAFT_77094

LBC:	LACBIDRAFT_188460

MPR:	MPER_01026 MPER_10147

MRR:	Moror_13970 Moror_7758

CCI:	CC1G_03447

SCM:	SCHCODRAFT_66672

ABP:	AGABI1DRAFT113991(AGABI1DRAFT_113991)

ABV:	AGABI2DRAFT193828(AGABI2DRAFT_193828)

CPUT:

CONPUDRAFT_124515 CONPUDRAFT_126428 CONPUDRAFT_91182

SLA:	SERLADRAFT_363075 SERLADRAFT_469613

WSE:	WALSEDRAFT_38521

WIC:	J056_002950

UMA:	UMAG_04127

PFP:	PFL1_03818

MGL:

MGL_3391

PGR:	PGTG_02889

MLR:	MELLADRAFT_36679

DDI:	DDB_G0281487 DDB_G0286833

DPP:	DICPUDRAFT_79373 DICPUDRAFT_86658

DFA:	DFA_04893 DFA_06128

ACAN:

ACA1_115740 ACA1_169090 ACA1_203130

SPAR:

SPRG_01499

NGR:	NAEGRDRAFT_2444

» show all

Reference

1 [PMID:7430141]

Authors

Brady DR, Crowder RD, Hayes WJ.

Title

Mixed function oxidases in sterol metabolism. Source of reducing equivalents.

Journal

J Biol Chem 255:10624-9 (1980)

Reference

2 [PMID:4401584]

Authors

Rahimtula AD, Gaylor JL.

Title

Partial purification of a microsomal sterol 4 -carboxylic acid decarboxylase.

Journal

J Biol Chem 247:9-15 (1972)

Reference

3 [PMID:9811880]

Authors

Gachotte D, Barbuch R, Gaylor J, Nickel E, Bard M

Title

Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis.

Journal

Proc Natl Acad Sci U S A 95:13794-9 (1998)
DOI:10.1073/pnas.95.23.13794

Sequence

[sce:YGL001C]

Reference

4 [PMID:14506130]

Authors

Caldas H, Herman GE

Title

NSDHL, an enzyme involved in cholesterol biosynthesis, traffics through the Golgi and accumulates on ER membranes and on the surface of lipid droplets.

Journal

Hum Mol Genet 12:2981-91 (2003)
DOI:10.1093/hmg/ddg321

Sequence

[hsa:50814]

Other DBs

ExplorEnz - The Enzyme Database:

1.1.1.170

IUBMB Enzyme Nomenclature:

1.1.1.170

ExPASy - ENZYME nomenclature database:

1.1.1.170

BRENDA, the Enzyme Database:

1.1.1.170

CAS:	71822-23-6

DBGET integrated database retrieval system