KEGG   ENZYME: 1.1.1.203Help
Entry
EC 1.1.1.203                Enzyme                                 

Name
uronate dehydrogenase;
uronate:NAD-oxidoreductase;
uronic acid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
uronate:NAD+ 1-oxidoreductase
Reaction(IUBMB)
(1) beta-D-galacturonate + NAD+ = D-galactaro-1,5-lactone + NADH + H+ [RN:R10841];
(2) beta-D-glucuronate + NAD+ = D-glucaro-1,5-lactone + NADH + H+ [RN:R10842]
Reaction(KEGG)
Substrate
beta-D-galacturonate;
NAD+ [CPD:C00003];
beta-D-glucuronate
Product
D-galactaro-1,5-lactone [CPD:C20889];
NADH [CPD:C00004];
H+ [CPD:C00080];
D-glucaro-1,5-lactone [CPD:C20890]
Comment
Requires Mg2+. The enzyme, characterized from the bacterium Agrobacterium fabrum, participates in oxidative degradation pathways for galacturonate and glucuronate. The enzyme can only accept the beta anomeric form of the substrate [4]. The 1,5-lactone product is rather stable at cytosolic pH and does not hydrolyse spontaneously at a substantial rate.
History
EC 1.1.1.203 created 1972 as EC 1.2.1.35, transferred 1984 to EC 1.1.1.203, modified 2014
Pathway
ec00053  Ascorbate and aldarate metabolism
Orthology
K18981  uronate dehydrogenase
Genes
PMY: Pmen_3299
PMK: MDS_3626
PRE: PCA10_06290(udh)
PPU: PP_1171(udh)
PPF: Pput_1205 Pput_1875
PPG: PputGB1_4240
PPW: PputW619_1189
PPB: PPUBIRD1_1216 PPUBIRD1_1845
PPI: YSA_07420 YSA_08930
PPX: T1E_2063 T1E_2983 T1E_4864
PPUN: PP4_11170(udh) PP4_20650(udh)
PSB: Psyr_0902
PSYR: N018_21145
PFB: VO64_1030
PMAN: OU5_6132
PSTT: CH92_11110
PSOS: POS17_4654
PANR: A7J50_3606
PSIL: PMA3_15595
CSA: Csal_2474
HEL: HELO_3405(udh)
HAM: HALO3641
SALN: SALB1_0476
RSO: RSp0831
RSN: RSPO_m01149(wcaG)
RSE: F504_4388
RPI: Rpic_4447
BUT: X994_6509
BTE: BTH_I0191
BTQ: BTQ_216
BTJ: BTJ_2269
BTZ: BTL_184
BTD: BTI_3529
BTV: BTHA_241
BTHE: BTN_1208
BTHM: BTRA_385
BTHA: DR62_1383
BTHL: BG87_348
BOK: DM82_3415
BOC: BG90_1403
BVE: AK36_4725
BCJ: BCAM0486
BCEN: DM39_4655
BCEW: DM40_3591
BCEO: I35_4379
BAM: Bamb_5236
BMU: Bmul_5164
BMK: DM80_4342
BMUL: NP80_3554
BCT: GEM_5230
BCED: DM42_4664
BDL: AK34_4888
BCON: NL30_23445
BUB: BW23_4239
BLAT: WK25_20560
BTEI: WS51_01235
BSEM: WJ12_22430
BPSL: WS57_11350
BMEC: WJ16_21460
BSTG: WT74_21665
BGU: KS03_1928
BUK: MYA_3657
BUL: BW21_15
PPNO: DA70_18130
PPNM: LV28_12655
PPUL: RO07_06635
PSPU: NA29_02505
PAPI: SG18_07025
AXX: ERS451415_05379(rmlB)
ODI: ODI_R0826
POL: Bpro_3109
PNA: Pnap_1591
AAV: Aave_2590
AAA: Acav_2613
VEI: Veis_1238
JAG: GJA_1432
HSE: Hsero_4608(wcaG)
HRB: Hrubri_4545(wcaG)
CFU: CFU_0232
CARE: LT85_0823
LCH: Lcho_1115
PBH: AAW51_4399(udh)
SME: SMc04145
SMER: DU99_00575
SMD: Smed_3319
ARA: Arad_9474
AVI: Avi_5694
RLE: pRL120778
RLG: Rleg_5176
SHZ: shn_22915
OAN: Oant_3405
OAH: DR92_3082
OCH: CES85_2646(udh)
BJA: blr2818
BRA: BRADO2488
BBT: BBta_2833
BRS: S23_51790
AOL: S58_51270
BRAD: BF49_1864
XAU: Xaut_3373
SNO: Snov_3256
MOR: MOC_3922
MAQU: Maq22A_c26490(wcaG)
MMED: Mame_00555(gerKI) Mame_00565
JAN: Jann_3497
RDE: RD1_2062
KVU: EIO_2319
KVL: KVU_1858
OTM: OSB_27000
RHC: RGUI_3939
TXI: TH3_13580
BWW: bwei_1507
BMYO: BG05_2577
PPM: PPSC2_17220(agmE)
PPO: PPM_3475(agmE)
PPOY: RE92_19875
PMW: B2K_01220
PRI: PRIO_1815
JEO: JMA_33520
SCO: SCO1896(SCI7.14)
SGR: SGR_5616
SGB: WQO_07265
SCT: SCAT_1075
SFA: Sfla_4918
SHY: SHJG_3347
SVE: SVEN_1530
STRP: F750_1764
SFI: SFUL_2918
SALU: DC74_2381
SALL: SAZ_13260
STRE: GZL_06626
SLD: T261_6270
SPRI: SPRI_5628
SLE: sle_52380(sle_52380)
STRD: NI25_29720
SMAL: SMALA_1848
SLAU: SLA_0609
SALJ: SMD11_5126
SFK: KY5_1806
MMAR: MODMU_4786
ACTI: UA75_23085
ACAD: UA74_22565
ASE: ACPL_3607
ACTS: ACWT_3479
CAI: Caci_7512
DRA: DR_A0222
DGE: Dgeo_2417
DFC: DFI_17465
 » show all
Taxonomy
Reference
1  [PMID:13657147]
  Authors
KILGORE WW, STARR MP.
  Title
Uronate oxidation by phytopathogenic pseudomonads.
  Journal
Nature 183:1412-3 (1959)
Reference
2  [PMID:19921179]
  Authors
Boer H, Maaheimo H, Koivula A, Penttila M, Richard P
  Title
Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene.
  Journal
Appl Microbiol Biotechnol 86:901-9 (2010)
DOI:10.1007/s00253-009-2333-9
  Sequence
[atu:Atu3143]
Reference
3  [PMID:22493433]
  Authors
Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P
  Title
Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate.
  Journal
J Biol Chem 287:17662-71 (2012)
DOI:10.1074/jbc.M111.335240
Reference
4  [PMID:21676870]
  Authors
Parkkinen T, Boer H, Janis J, Andberg M, Penttila M, Koivula A, Rouvinen J
  Title
Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens.
  Journal
J Biol Chem 286:27294-300 (2011)
DOI:10.1074/jbc.M111.254854
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.203
IUBMB Enzyme Nomenclature: 1.1.1.203
ExPASy - ENZYME nomenclature database: 1.1.1.203
BRENDA, the Enzyme Database: 1.1.1.203
CAS: 37250-98-9

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