Entry |
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Name |
uronate dehydrogenase;
uronate:NAD-oxidoreductase;
uronic acid dehydrogenase
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Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
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Sysname |
uronate:NAD+ 1-oxidoreductase
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Reaction(IUBMB) |
(1) beta-D-galacturonate + NAD+ = D-galactaro-1,5-lactone + NADH + H+ [RN: R10841];
(2) beta-D-glucuronate + NAD+ = D-glucaro-1,5-lactone + NADH + H+ [RN: R10842]
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Reaction(KEGG) |
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Substrate |
beta-D-galacturonate;
NAD+ [CPD: C00003];
beta-D-glucuronate
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Product |
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Comment |
Requires Mg2+. The enzyme, characterized from the bacterium Agrobacterium fabrum, participates in oxidative degradation pathways for galacturonate and glucuronate. The enzyme can only accept the beta anomeric form of the substrate [4]. The 1,5-lactone product is rather stable at cytosolic pH and does not hydrolyse spontaneously at a substantial rate.
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History |
EC 1.1.1.203 created 1972 as EC 1.2.1.35, transferred 1984 to EC 1.1.1.203, modified 2014
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Pathway |
ec00053 | Ascorbate and aldarate metabolism |
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Orthology |
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Genes |
» show all
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Reference |
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Authors |
KILGORE WW, STARR MP. |
Title |
Uronate oxidation by phytopathogenic pseudomonads. |
Journal |
Nature 183:1412-3 (1959) |
Reference |
|
Authors |
Boer H, Maaheimo H, Koivula A, Penttila M, Richard P |
Title |
Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene. |
Journal |
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Sequence |
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Reference |
|
Authors |
Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P |
Title |
Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate. |
Journal |
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Reference |
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Authors |
Parkkinen T, Boer H, Janis J, Andberg M, Penttila M, Koivula A, Rouvinen J |
Title |
Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens. |
Journal |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 1.1.1.203 |
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