KEGG   ENZYME: 1.1.1.272
Entry
EC 1.1.1.272                Enzyme                                 
Name
D-2-hydroxyacid dehydrogenase (NADP+);
ddh (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(R)-2-hydroxycarboxylate:NADP+ oxidoreductase
Reaction(IUBMB)
an (R)-2-hydroxycarboxylate + NADP+ = a 2-oxocarboxylate + NADPH + H+ [RN:R10528]
Reaction(KEGG)
R10528
Substrate
(R)-2-hydroxycarboxylate [CPD:C02489];
NADP+ [CPD:C00006]
Product
2-oxocarboxylate [CPD:C00161];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
This enzyme, characterized from the halophilic archaeon Haloferax mediterranei and the mold Aspergillus oryzae, catalyses a stereospecific reduction of 2-oxocarboxylic acids into the corresponding D-2-hydroxycarboxylic acids. The enzyme prefers substrates with a main chain of 5 carbons (such as 4-methyl-2-oxopentanoate) to those with a shorter chain, and can use NADH with much lower efficiency. cf. EC 1.1.1.345, (D)-2-hydroxyacid dehydrogenase (NAD+).
History
EC 1.1.1.272 created 2002, modified 2013
Orthology
K17064  D-2-hydroxyacid dehydrogenase (NADP+)
Genes
HDLHALDL1_11305
HHBHhub_1992(ddh1) Hhub_4287(ddh2)
HNOLT974_04880
HJEHacjB3_09750 HacjB3_13315 HacjB3_18943
SALRFQU85_03585
HAADMW046_10035 MW046_18195
HALXM0R89_08670 M0R89_21255
HAXZM0R88_08880
SALIL593_08725
HMArrnAC2451(serA5)
HHIHAH_2884(serA4)
HHNHISP_14660
HABSG26_06795
HTABVU17_14025
HALJG9465_05500
HSINKDQ40_15040
NMONmlp_2102(ddh)
NSALHWV07_07755
HRRHZS55_21155
HPELHZS54_24735
HDOMUK72_06440
HVOHVO_1932(ddh)
HMEHFX_2024(serA5)
HGIABY42_09365
HALEG3A49_12650
HLSKU306_02870
HBOHbor_08320
HAQDU484_03700 DU484_04865
HAJDU500_04185 DU500_05305
HAERDU502_01655 DU502_02695
HRAEI982_10885 EI982_11715
HLMDV707_07845 DV707_14950
HALMFCF25_00735
HALQHrr1229_010840 Hrr1229_013535
SRUBC2R22_17135
HALNB4589_002660
HALGHUG10_07745 HUG10_15100
HALUHUG12_08505 HUG12_18220 HUG12_19825
HREK6T36_14320 K6T36_17835
HDFAArcSl_1274(ddh)
HAHHalar_1750
HTUHtur_3563
HDABB347_11980
HSALJMJ58_10540
HLOJ0X27_12185
HAKZJ0X25_05510
NMGNmag_1575(ddh)
HXAHalxa_3414
NATNJ7G_2895
NPENatpe_0262
NVRFEJ81_01465
NPLFGF80_09520
NAYHYG81_09300
HALYHYG82_12060
HJTDVR14_10685
NOUNatoc_2479 Natoc_4312
HLRHALLA_18410
HLCCHINAEXTREME06950
NAJB1756_05180
NAGAArcMg_0286
NANAArc1_0296
NBGDV706_06100
NASGCU68_07775
SAWLNGM29_00740
 » show all
Reference
1  [PMID:17049749]
  Authors
Domenech J, Ferrer J
  Title
A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression.
  Journal
Biochim Biophys Acta 1760:1667-74 (2006)
DOI:10.1016/j.bbagen.2006.08.024
  Sequence
[hme:HFX_2024]
Reference
2  [PMID:26615399]
  Authors
Shimizu M, Yamamoto T, Okabe N, Sakai K, Koide E, Miyachi Y, Kurimoto M, Mochizuki M, Yoshino-Yasuda S, Mitsui S, Ito A, Murano H, Takaya N, Kato M
  Title
Novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the Japanese sake flavor, ethyl leucate.
  Journal
Appl Microbiol Biotechnol 100:3137-45 (2016)
DOI:10.1007/s00253-015-7182-0
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.272
IUBMB Enzyme Nomenclature: 1.1.1.272
ExPASy - ENZYME nomenclature database: 1.1.1.272
BRENDA, the Enzyme Database: 1.1.1.272
CAS: 81210-65-3

DBGET integrated database retrieval system