KEGG ENZYME: 1.1.1.272

ENZYME: 1.1.1.272

Entry

EC 1.1.1.272                Enzyme

Name

D-2-hydroxyacid dehydrogenase (NADP+);
ddh (gene name)

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

(R)-2-hydroxycarboxylate:NADP+ oxidoreductase

Reaction(IUBMB)

an (R)-2-hydroxycarboxylate + NADP+ = a 2-oxocarboxylate + NADPH + H+ [RN:R10528]

Reaction(KEGG)

R10528

Substrate

(R)-2-hydroxycarboxylate [CPD:C02489];
NADP+ [CPD:C00006]

Product

2-oxocarboxylate [CPD:C00161];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

This enzyme, characterized from the halophilic archaeon Haloferax mediterranei and the mold Aspergillus oryzae, catalyses a stereospecific reduction of 2-oxocarboxylic acids into the corresponding D-2-hydroxycarboxylic acids. The enzyme prefers substrates with a main chain of 5 carbons (such as 4-methyl-2-oxopentanoate) to those with a shorter chain, and can use NADH with much lower efficiency. cf. EC 1.1.1.345, (D)-2-hydroxyacid dehydrogenase (NAD+).

History

EC 1.1.1.272 created 2002, modified 2013

Orthology

K17064

D-2-hydroxyacid dehydrogenase (NADP+)

Genes

HDL:	HALDL1_11305

HHB:	Hhub_1992(ddh1) Hhub_4287(ddh2)

HNO:	LT974_04880

HAHS:

HSRCO_0596(serA3)

SALR:

FQU85_03585

HJE:	HacjB3_09750 HacjB3_13315 HacjB3_18943

HDO:	MUK72_06440

HALX:

M0R89_08670 M0R89_21255

HAXZ:

M0R88_08880

HAYC:

NGM10_09975

HAXY:

NGM07_14510

SALI:

L593_08725

HMA:	rrnAC2451(serA5)

HHI:	HAH_2884(serA4)

HHN:	HISP_14660

HAB:	SG26_06795

HTA:	BVU17_14025

HALJ:

G9465_05500

HSIN:

KDQ40_15040

NMO:	Nmlp_2102(ddh)

NSAL:

HWV07_07755

HRR:	HZS55_21155

HPEL:

HZS54_24735

HAAD:

MW046_10035 MW046_18195

HVO:	HVO_1932(ddh)

HALE:

G3A49_12650

HLN:	SVXHx_2687(ddh2)

HME:	HFX_2024(serA5)

HGI:	ABY42_09365

HLS:	KU306_02870

HBO:	Hbor_08320

HAQ:	DU484_03700 DU484_04865

HAJ:	DU500_04185 DU500_05305

HAER:

DU502_01655 DU502_02695

HRA:	EI982_10885 EI982_11715

HLM:	DV707_07845 DV707_14950

HALM:

FCF25_00735

HALQ:

Hrr1229_010840 Hrr1229_013535

SRUB:

C2R22_17135

HALN:

B4589_002660

HALG:

HUG10_07745 HUG10_15100

HALU:

HUG12_08505 HUG12_18220 HUG12_19825

HRE:	K6T36_14320 K6T36_17835

HDF:	AArcSl_1274(ddh)

HAH:	Halar_1750

HTU:

Htur_3563

HSAL:

JMJ58_10540

HAKZ:

J0X25_05510

NMG:	Nmag_1575(ddh)

HXA:	Halxa_3414

NAT:

NJ7G_2895

NPE:	Natpe_0262

NVR:	FEJ81_01465

NPL:	FGF80_09520

NAY:	HYG81_09300

HALY:

HYG82_12060

HJT:	DVR14_10685

HLO:	J0X27_12185

SAWL:

NGM29_00740

HALV:

NGM15_10270

NOU:	Natoc_2479 Natoc_4312

HLR:	HALLA_18410

HLC:	CHINAEXTREME06950

NAJ:	B1756_05180

NAG:	AArcMg_0286

NAN:	AArc1_0296

NARA:

QQ977_11315(ddh)

NBG:	DV706_06100

NAS:	GCU68_07775

HDA:	BB347_11980

» show all

Reference

1 [PMID:17049749]

Authors

Domenech J, Ferrer J

Title

A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression.

Journal

Biochim Biophys Acta 1760:1667-74 (2006)
DOI:10.1016/j.bbagen.2006.08.024

Sequence

[hme:HFX_2024]

Reference

2 [PMID:26615399]

Authors

Shimizu M, Yamamoto T, Okabe N, Sakai K, Koide E, Miyachi Y, Kurimoto M, Mochizuki M, Yoshino-Yasuda S, Mitsui S, Ito A, Murano H, Takaya N, Kato M

Title

Novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the Japanese sake flavor, ethyl leucate.

Journal

Appl Microbiol Biotechnol 100:3137-45 (2016)
DOI:10.1007/s00253-015-7182-0

Other DBs

ExplorEnz - The Enzyme Database:

1.1.1.272

IUBMB Enzyme Nomenclature:

1.1.1.272

ExPASy - ENZYME nomenclature database:

1.1.1.272

BRENDA, the Enzyme Database:

1.1.1.272

CAS:	81210-65-3

DBGET integrated database retrieval system