KEGG ENZYME: 1.1.1.299

ENZYME: 1.1.1.299

Entry

EC 1.1.1.299                Enzyme

Name

malate dehydrogenase [NAD(P)+];
MdH II, NAD(P)+-dependent malate dehyrogenase

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

(S)-malate:NAD(P)+ oxidoreductase

Reaction(IUBMB)

(S)-malate + NAD(P)+ = oxaloacetate + NAD(P)H + H+ [RN:R00342 R00343]

Reaction(KEGG)

R00342 R00343

Substrate

(S)-malate [CPD:C00149];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

oxaloacetate [CPD:C00036];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

This enzyme, which was characterized from the methanogenic archaeon Methanobacterium thermoautotrophicum, catalyses only the reduction of oxaloacetate, and can use NAD+ and NADP+ with similar specific activity [1].
Different from EC 1.1.1.37 (malate dehydrogenase (NAD+)), EC 1.1.1.82 (malate dehydrogenase (NADP+)) and EC 1.1.5.4 (malate dehydrogenase (quinone)).

History

EC 1.1.1.299 created 2009

Reference

1 [PMID:9639601]

Authors

Thompson H, Tersteegen A, Thauer RK, Hedderich R

Title

Two malate dehydrogenases in Methanobacterium thermoautotrophicum.

Journal

Arch Microbiol 170:38-42 (1998)
DOI:10.1007/s002030050612

Sequence

[mmg:MTBMA_c06400]