KEGG   ENZYME: 1.1.1.328Help
Entry
EC 1.1.1.328                Enzyme                                 

Name
nicotine blue oxidoreductase;
nboR (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3,3'-bipyridine-2,2',5,5',6,6'-hexol:NADP+ 11-oxidoreductase
Reaction(IUBMB)
3,3'-bipyridine-2,2',5,5',6,6'-hexol + NAD(P)+ = (E)-2,2',5,5'-tetrahydroxy-6H,6'H-[3,3'-bipyridinylidene]-6,6'-dione + NAD(P)H + H+ [RN:R10131 R10132]
Reaction(KEGG)
Substrate
3,3'-bipyridine-2,2',5,5',6,6'-hexol [CPD:C20381];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
(E)-2,2',5,5'-tetrahydroxy-6H,6'H-[3,3'-bipyridinylidene]-6,6'-dione [CPD:C16152];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme, characterized from the nicotine degrading bacterium Arthrobacter nicotinovorans, catalyses the reduction of "nicotine blue" to its hydroquinone form (the opposite direction from that shown). Nicotine blue is the name given to the compound formed by the autocatalytic condensation of two molecules of 2,3,6-trihydroxypyridine, an intermediate in the nicotine degradation pathway. The main role of the enzyme may be to prevent the intracellular formation of nicotine blue semiquinone radicals, which by redox cycling would lead to the formation of toxic reactive oxygen species. The enzyme possesses a slight preference for NADH over NADPH.
History
EC 1.1.1.328 created 2012
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K19190  nicotine blue oxidoreductase
Genes
VFF: VITFI_CDS2845
ADT: APT56_16420
MELM: C7H73_07205
BPRC: D521_0899
RHP: LPB142_08720
PYE: A6J80_04430
MSA: Mycsm_01324
MMC: Mmcs_1283
MKM: Mkms_1300
MJL: Mjls_1312
MPHL: MPHLCCUG_01580(nboR)
MVQ: MYVA_1419
MABB: MASS_2872
MABO: NF82_14695
MCHE: BB28_14795
MSTE: MSTE_02906
NSR: NS506_00221(nboR)
REQ: REQ_32150
RHB: NY08_1722
RFA: A3L23_00084(nboR)
RHS: A3Q41_03330(nboR)
GOR: KTR9_1746
SCO: SCO6244(SCAH10.09c)
SGR: SGR_1296
SGB: WQO_28770
SCT: SCAT_4783
SFA: Sfla_1035
SBH: SBI_02940
SHY: SHJG_7292
SVE: SVEN_6103
SALB: XNR_0568
SALS: SLNWT_0575
STRP: F750_5812
SFI: SFUL_6187
SALU: DC74_6410
SALL: SAZ_32200
STRE: GZL_02602
SLD: T261_1838
STRM: M444_27230
SAMB: SAM23877_5968(nboR)
SPRI: SPRI_1441
SRW: TUE45_06858(nboR)
SLE: sle_14380(sle_14380)
SRN: A4G23_04834(nboR)
STRD: NI25_08170
SMAL: SMALA_6028
SLAU: SLA_6175
SALF: SMD44_06875(mocA)
SALJ: SMD11_1426(mocA)
SLX: SLAV_08700(nboR)
SFK: KY5_6604c
KSK: KSE_19540
ART: Arth_3415
ARM: ART_0495
ARX: ARZXY2_335(mocA) ARZXY2_4571(mocA)
AAU: AAur_3392
ACH: Achl_3196
LMOI: VV02_15020
SERJ: SGUI_0302
NDK: I601_0524(nboR)
PSIM: KR76_21425
NDA: Ndas_0865
NAL: B005_3768
TCU: Tcur_2977
SRO: Sros_2846
ACE: Acel_1630
MMAR: MODMU_5453
SEN: SACE_1257
AMD: AMED_1865
AMN: RAM_09465
AMM: AMES_1851
AMZ: B737_1852
AOI: AORI_6015
PDX: Psed_5731
PSEA: WY02_13890
PSEE: FRP1_28645
PSEH: XF36_27900
AMI: Amir_5126
SESP: BN6_62010
KAL: KALB_3764
ALL: CRK56388
SAQ: Sare_0199
MIL: ML5_4099
ACTN: L083_6390
AFS: AFR_32855
CAI: Caci_1881
SNA: Snas_2534
TBI: Tbis_1386
CTHE: Chro_2160
GBA: J421_2158
 » show all
Taxonomy
Reference
1  [PMID:17293530]
  Authors
Mihasan M, Chiribau CB, Friedrich T, Artenie V, Brandsch R
  Title
An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism.
  Journal
Appl Environ Microbiol 73:2479-85 (2007)
DOI:10.1128/AEM.02668-06
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.328
IUBMB Enzyme Nomenclature: 1.1.1.328
ExPASy - ENZYME nomenclature database: 1.1.1.328
BRENDA, the Enzyme Database: 1.1.1.328

DBGET integrated database retrieval system