KEGG   ENZYME: 1.1.1.328
Entry
EC 1.1.1.328                Enzyme                                 
Name
nicotine blue oxidoreductase;
nboR (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3,3'-bipyridine-2,2',5,5',6,6'-hexol:NADP+ 11-oxidoreductase
Reaction(IUBMB)
3,3'-bipyridine-2,2',5,5',6,6'-hexol + NAD(P)+ = (E)-2,2',5,5'-tetrahydroxy-6H,6'H-[3,3'-bipyridinylidene]-6,6'-dione + NAD(P)H + H+ [RN:R10131 R10132]
Reaction(KEGG)
R10131 R10132
Substrate
3,3'-bipyridine-2,2',5,5',6,6'-hexol [CPD:C20381];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
(E)-2,2',5,5'-tetrahydroxy-6H,6'H-[3,3'-bipyridinylidene]-6,6'-dione [CPD:C16152];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme, characterized from the nicotine degrading bacterium Arthrobacter nicotinovorans, catalyses the reduction of "nicotine blue" to its hydroquinone form (the opposite direction from that shown). Nicotine blue is the name given to the compound formed by the autocatalytic condensation of two molecules of 2,3,6-trihydroxypyridine, an intermediate in the nicotine degradation pathway. The main role of the enzyme may be to prevent the intracellular formation of nicotine blue semiquinone radicals, which by redox cycling would lead to the formation of toxic reactive oxygen species. The enzyme possesses a slight preference for NADH over NADPH.
History
EC 1.1.1.328 created 2012
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K19190  nicotine blue oxidoreductase
Genes
COKCOCCU_07140(nboR)
ARTArth_3415
ARRARUE_c35280
ARZAUT26_16235
ARUASPU41_03860
ARQBWQ92_13935
ARNCGK93_19020
ARXARZXY2_335(mocA) ARZXY2_4571(mocA)
ARTHC3B78_16675
AJGKKR91_14055
ASUNKG104_14905
ASUFMNQ99_08130
ARTYAHiyo4_43900
AAUAAur_3392
PUEFV140_17985
PNVJMY29_16225 JMY29_20595
ACHAchl_3196
APNAsphe3_33350
PSULAU252_13775
PSNINIBR502771_08360
PSEYGU243_12945
FTJFTUN_0605
 » show all
Reference
1  [PMID:17293530]
  Authors
Mihasan M, Chiribau CB, Friedrich T, Artenie V, Brandsch R
  Title
An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism.
  Journal
Appl Environ Microbiol 73:2479-85 (2007)
DOI:10.1128/AEM.02668-06
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.328
IUBMB Enzyme Nomenclature: 1.1.1.328
ExPASy - ENZYME nomenclature database: 1.1.1.328
BRENDA, the Enzyme Database: 1.1.1.328

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