KEGG   ENZYME: 1.1.1.335
Entry
EC 1.1.1.335                Enzyme                                 

Name
UDP-N-acetyl-2-amino-2-deoxyglucuronate dehydrogenase;
WlbA;
WbpB
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
UDP-N-acetyl-2-amino-2-deoxy-alpha-D-glucuronate:NAD+ 3-oxidoreductase
Reaction(IUBMB)
UDP-N-acetyl-2-amino-2-deoxy-alpha-D-glucuronate + NAD+ = UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ [RN:R10140]
Reaction(KEGG)
R10140
Substrate
UDP-N-acetyl-2-amino-2-deoxy-alpha-D-glucuronate [CPD:C04573];
NAD+ [CPD:C00003]
Product
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate [CPD:C20395];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This enzyme participates in the biosynthetic pathway for UDP-alpha-D-ManNAc3NAcA (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronic acid), an important precursor of B-band lipopolysaccharide. The enzymes from Pseudomonas aeruginosa serotype O5 and Thermus thermophilus form a complex with the the enzyme catalysing the next step the pathway (EC 2.6.1.98, UDP-2-acetamido-2-deoxy-ribo-hexuluronate aminotransferase). The enzyme also possesses an EC 1.1.99.2 (L-2-hydroxyglutarate dehydrogenase) activity, and utilizes the 2-oxoglutarate produced by EC 2.6.1.98 to regenerate the tightly bound NAD+. The enzymes from Bordetella pertussis and Chromobacterium violaceum do not bind NAD+ as tightly and do not require 2-oxoglutarate to function.
History
EC 1.1.1.335 created 2012
Pathway
ec00520  Amino sugar and nucleotide sugar metabolism
ec00541  O-Antigen nucleotide sugar biosynthesis
ec01100  Metabolic pathways
Orthology
K13016  UDP-N-acetyl-2-amino-2-deoxyglucuronate dehydrogenase
K13020  UDP-N-acetyl-2-amino-2-deoxyglucuronate dehydrogenase
Genes
CBRA: A6J81_14380
CWE: CO701_19350
CAMA: F384_24285
CFAR: CI104_22315
BUF: D8682_19095
BAGE: BADSM9389_06600
SMAF: D781_0082
RAH: Rahaq_1175
RAQ: Rahaq2_1252
RAA: Q7S_05670
SOD: Sant_2703
PLU: plu4797(wblB)
PAY: PAU_04312(wblB)
XBO: XBJ1_4314
XNE: XNC1_0112
XNM: XNC2_0114
LRI: NCTC12151_03551(gfo)
MSU: MS1500(mviM)
ASU: Asuc_0110
AVT: NCTC3438_01791(gfo_2)
PAE: PA3158(wbpB)
PAEV: N297_3269(wbpB)
PAEI: N296_3269(wbpB)
PAEM: U769_09132
PAEC: M802_3267(wbpB)
PAEO: M801_3134(wbpB)
PMY: Pmen_1858
PPUH: B479_06900
PSES: PSCI_2866
PSOS: POS17_4987
PSET: THL1_1977
ACB: A1S_0053
SAZ: Sama_2259
SSE: Ssed_2972
PSEO: OM33_04950
MARJ: MARI_04640(wbpB)
AAUS: EP12_05245
ASP: AOR13_496
SDE: Sde_0872
LFA: LFA_0788
LOK: Loa_02107
LSS: NCTC12082_00067(gfo)
TNI: TVNIR_0687(pht4_[H])
HCO: LOKO_02148(wbpB)
ADI: B5T_01708(wbpB)
NZO: SAMEA4504057_0083(iolG)
CVI: CV_4127
CHAE: CH06BL_04730(wlbA) CH06BL_39690(wlbA)
LHK: LHK_02805(wblB)
PSE: NH8B_0413
AMAH: DLM_4446
REH: H16_B0033(wbpB)
PPNO: DA70_17270
LMIR: NCTC12852_00294(afr_1)
BPE: BP0093(bplA)
BPC: BPTD_0089(bplA)
BPER: BN118_0162(wlbA)
BPET: B1917_0096(bplA)
BPEU: Q425_2060(bplA)
BPAR: BN117_0153(wlbA)
BPA: BPP0155(bplA)
BBH: BN112_3256(wlbA)
BBR: BB0155(bplA)
BBM: BN115_0144(wlbA)
BBX: BBS798_0154(bplA)
BPT: Bpet4814(wlbA)
BAV: BAV0098(wlbA)
BHO: D560_1328
BHM: D558_1317
BHZ: ACR54_00110(gfo)
BTRM: SAMEA390648701342(bplA)
AXX: ERS451415_06023(gfo)
TEA: KUI_0407
TEG: KUK_0147
ODI: ODI_R2826
PNA: Pnap_3129
AAV: Aave_0950
AJS: Ajs_3015
CTES: O987_08010
HYB: Q5W_21470
LCH: Lcho_0289
RGE: RGE_41570(bplA)
HPJ: jhp_0620
HPG: HPG27_637
HPP: HPP12_0690(wbpB)
HPL: HPB8_881(wbpB)
HPI: hp908_0688(wbpB)
HPQ: hp2017_0665(wbpB)
HPW: hp2018_0666(wbpB)
HEF: HPF16_0688(wbpB)
HPF: HPF30_0654(wbpB)
HEQ: HPF32_0643(wbpB)
HEX: HPF57_0700(wbpB)
HPZ: HPKB_0669
HPD: KHP_0646(wbpB)
HEY: MWE_0830(wbpB)
HPYO: HPOK113_0689(wbpB)
HPYL: HPOK310_0668(wbpB)
HPYR: K747_07485
HPYI: K750_05685
HPYU: K751_03995
HPYM: K749_02110
HEB: U063_0983
HEZ: U064_0987
HHE: HH_1662(wbpB)
HAC: Hac_0861(wbpB)
HMS: HMU06630
HCE: HCW_05405
HCM: HCD_05220
HCP: HCN_0856(wbpB)
HCB: HCBAA847_1124(wbpB)
HCL: NCTC13205_00267(ycjS)
WSU: WS2188(WBPB)
CBLA: CBLAS_0711
ABU: Abu_0662
AAQI: AAQM_2034
ASUI: ASUIS_2001
HBV: ABIV_0864
PACO: AACT_0950
SUN: SUN_0093
GLO: Glov_1510
GEB: GM18_1433
LIP: LIC097
LIR: LAW_30095
DBA: Dbac_3255
DOL: Dole_1819
DAL: Dalk_1674
MES: Meso_4484
SMER: DU99_28765
SMD: Smed_4750
RHT: NT26_2973
BRS: S23_22680
RUT: FIU92_10720(pht2)
KVU: EIO_3032
PSF: PSE_p0069
OTM: OSB_21250(gfo_3)
MAG: amb0131
MGRY: MSR1_31310(ycjS_1)
MAGX: XM1_0970
MAGQ: MGMAQ_3670
MGM: Mmc1_2424
BBAT: Bdt_1672
BBAC: EP01_04555
BLI: BL03076
BLD: BLi00809(yrbE)
BLH: BaLi_c09130(yrbE)
BSON: S101395_01005(bvdR)
BACW: QR42_02065
BEO: BEH_14640
BGY: BGLY_0828
BFD: NCTC4823_03739(gfo)
BCL: ABC0605
GKA: GK3166
AAMY: GFC30_481
AXL: AXY_03830
PSYO: PB01_04620
SSP: SSP1334
ECAS: ECBG_00089
CTC: CTC_02264
CBA: CLB_3136
CBH: CLC_3009
CBY: CLM_3513
CBL: CLK_2501
CBK: CLL_A3174
CBB: CLD_1433
CBI: CLJ_B3373
CBN: CbC4_0993
CBT: CLH_2923
CBF: CLI_3166
CBM: CBF_3155
CBE: Cbei_4619
CBZ: Cbs_4619
CBEI: LF65_05162
CKL: CKL_3168
CKR: CKR_2806
CPAS: Clopa_4712
CSB: CLSA_c41240(yrbE2)
CSQ: CSCA_4789
CTYK: CTK_C08660
CCOH: SAMEA4530647_1933(ycjS)
AMT: Amet_3444
HHW: NCTC503_02000(afr)
CCEL: CCDG5_0711
FPR: FP2_24960
BPB: bpr_I0406
SWO: Swol_0706
SGY: Sgly_3009
ELM: ELI_2560
SAY: TPY_3674
TIT: Thit_0178
TKI: TKV_c06640(mviM)
TSH: Tsac_0498
TOC: Toce_1590
FMA: FMG_0585
APR: Apre_1597
PUF: UFO1_0304
LPIL: LIP_1016
CGB: cg2918
CGU: WA5_2546
CGM: cgp_2918
CGJ: AR0_12795
CAMG: CAMM_03405
RER: RER_46960
REY: O5Y_22175
ROP: ROP_15350
SALU: DC74_7991
SALL: SAZ_41245
STRE: GZL_p00135
LXX: Lxx17360(bplA)
CMI: CMM_2271
CMS: CMS2465
CMC: CMN_02246
MOY: CVS54_03687(iolX)
ARM: ART_1531
AAU: AAur_0525
ACH: Achl_0779
MPH: MLP_05720
AFS: AFR_16830
CWO: Cwoe_4459
ELE: Elen_0638
RCA: Rcas_2202
TRA: Trad_1846
TTH: TT_C0287(wbpB)
MRB: Mrub_0358
OTE: Oter_0898
OBG: Verru16b_02254(afr_3)
CAA: Caka_2925
RBA: RB6199
PIR: VN12_21635(afr_4)
RUL: UC8_06010(wbpB_1) UC8_10100(gfo_3) UC8_50390(wbpB_2)
MFF: MFFC18_27070(gfo_4)
TTF: THTE_2673
PLM: Plim_2198
PEH: Spb1_29720(afr_3)
PLS: VT03_19395(afr_3) VT03_23415(ycjS_2)
PLH: VT85_17850(gfo_3)
FMR: Fuma_02244(iolG_9) Fuma_06237(afr_5)
GMR: GmarT_05450(afr_1)
BVO: Pan97_25430(gfo_5)
GES: VT84_01060(gfo_1) VT84_03575(ycjS)
IPA: Isop_0786
PBOR: BSF38_01063(gfo_2) BSF38_03488(gfo_6)
AGV: OJF2_28930(gfo_7)
KST: KSMBR1_2318(cbaC_1)
PBAS: SMSP2_00900(ycjS_4) SMSP2_01883(wbpB)
ALUS: STSP2_02795(wbpB)
TPED: TPE_0183
SUS: Acid_1518
ABAC: LuPra_02895(afr_4)
TLI: Tlie_1823
GAU: GAU_1966
GBA: J421_2473
PGI: PG_2119
PGN: PGN_0168(wbpB) PGN_1068
PCRE: NCTC12858_00136(iolG)
PDI: BDI_2789
TFO: BFO_1077
BACC: BRDCF_p620(ycjS) BRDCF_p621
BLQ: L21SP5_01622(wbpB)
RMR: Rmar_1674
SGN: SGRA_4064
SLI: Slin_4507
FAE: FAES_5182
HSW: Hsw_1635
TJE: TJEJU_1135(wbpB)
TMAR: MARIT_2536(wbpB)
SPON: HME9304_01540(wbpB)
RAI: RA0C_1443
RAR: RIA_1052
RAG: B739_0706
RAE: G148_0432
RAT: M949_1164
CTAK: 4412677_01091(yvaA)
IAL: IALB_2460(wbpB)
MRO: MROS_2688
CPRV: CYPRO_3324
CACI: CLOAM1565(mviM)
TMW: THMA_0600
TMQ: THMB_0600
TMX: THMC_0600
TPT: Tpet_0333
TRQ: TRQ2_0351
TNA: CTN_0079
TNP: Tnap_0367
TLE: Tlet_0884
TME: Tmel_1052
TAF: THA_1334
THER: Y592_06070
FNO: Fnod_1779
PMO: Pmob_0962
MARN: LN42_07805
DTN: DTL3_0340
KOL: Kole_1641
ASAC: ATHSA_0559
CABY: Cabys_972
CTHI: THC_1547
HUT: Huta_2849
HTI: HTIA_2755
HTU: Htur_4428
SSOA: SULA_0826
SSOL: SULB_0828
SSOF: SULC_0826
SID: M164_2311
SII: LD85_2596
SIH: SiH_2250
SIR: SiRe_2194
SIC: SiL_2159
CMA: Cmaq_0434
 » show all
Reference
1  [PMID:19282284]
  Authors
Westman EL, McNally DJ, Charchoglyan A, Brewer D, Field RA, Lam JS
  Title
Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa.
  Journal
J Biol Chem 284:11854-62 (2009)
DOI:10.1074/jbc.M808583200
  Sequence
[pae:PA3158]
Reference
2  [PMID:19348502]
  Authors
Larkin A, Imperiali B
  Title
Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1.
  Journal
Biochemistry 48:5446-55 (2009)
DOI:10.1021/bi900186u
  Sequence
[pae:PA3158]
Reference
3  [PMID:20690587]
  Authors
Thoden JB, Holden HM
  Title
Structural and functional studies of WlbA: A dehydrogenase involved in the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid .
  Journal
Biochemistry 49:7939-48 (2010)
DOI:10.1021/bi101103s
  Sequence
[pae:PA3158]
Reference
4  [PMID:21241053]
  Authors
Thoden JB, Holden HM
  Title
Biochemical and structural characterization of WlbA from Bordetella pertussis and Chromobacterium violaceum: enzymes required for the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid.
  Journal
Biochemistry 50:1483-91 (2011)
DOI:10.1021/bi101871f
  Sequence
[bpe:BP0093] [cvi:CV_4127]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.335
IUBMB Enzyme Nomenclature: 1.1.1.335
ExPASy - ENZYME nomenclature database: 1.1.1.335
BRENDA, the Enzyme Database: 1.1.1.335

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