| Entry |
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| Name |
2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming);
2,5-diketo-D-gluconate-reductase (ambiguous);
YqhE reductase;
dkgA (gene name);
dkgB (gene name)
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| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
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| Sysname |
2-dehydro-D-gluconate:NADP+ 2-oxidoreductase (2-dehydro-L-gulonate-forming)
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| Reaction(IUBMB) |
2-dehydro-L-gulonate + NADP+ = 2,5-didehydro-D-gluconate + NADPH + H+ [RN: R08878]
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| Reaction(KEGG) |
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| Substrate |
2-dehydro-L-gulonate;
NADP+ [CPD: C00006]
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| Product |
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| Comment |
The enzyme is involved in ketogluconate metabolism, and catalyses the reaction in vivo in the reverse direction to that shown [1]. It is used in the commercial microbial production of ascorbate. cf. EC 1.1.1.274, 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming).
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| History |
EC 1.1.1.346 created 2013
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| Orthology |
| K06221 | 2,5-diketo-D-gluconate reductase A |
| K06222 | 2,5-diketo-D-gluconate reductase B |
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| Genes |
» show all
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| Reference |
1 |
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| Authors |
Sonoyama, T. and Kobayashi, K. |
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| Title |
Purification and properties of two 2,5-diketo-D-gluconate reductases from a mutant strain derived from Corynebacterium sp. |
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| Journal |
J Ferment Technol 65:311-317 (1987) |
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| Reference |
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| Authors |
Miller JV, Estell DA, Lazarus RA |
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| Title |
Purification and characterization of 2,5-diketo-D-gluconate reductase from Corynebacterium sp. |
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| Journal |
J Biol Chem 262:9016-20 (1987) |
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| Sequence |
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| Reference |
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| Authors |
Yum DY, Lee BY, Pan JG. |
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| Title |
Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli. |
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| Journal |
Appl Environ Microbiol 65:3341-6 (1999) |
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| Reference |
4 |
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| Authors |
Maremonti, M., Greco, G. and Wichmann, R. |
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| Title |
Characterisation of 2,5-diketo-D-gluconic acid reductase from Corynebacterium sp. |
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| Journal |
Biotechnology Letters 18:845-850 (1996) |
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| Reference |
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| Authors |
Khurana S, Powers DB, Anderson S, Blaber M |
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| Title |
Crystal structure of 2,5-diketo-D-gluconic acid reductase A complexed with NADPH at 2.1-A resolution. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 1.1.1.346 |
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