KEGG   ENZYME: 1.1.1.348Help
Entry
EC 1.1.1.348                Enzyme                                 

Name
(3R)-2'-hydroxyisoflavanone reductase;
vestitone reductase;
pterocarpin synthase (incorrect);
pterocarpan synthase (incorrect)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(3R)-2'-hydroxyisoflavanone:NADP+ 4-oxidoreductase
Reaction(IUBMB)
a (4R)-4,2'-dihydroxyisoflavan + NADP+ = a (3R)-2'-hydroxyisoflavanone + NADPH + H+ [RN:R11877]
Reaction(KEGG)
Substrate
(4R)-4,2'-dihydroxyisoflavan [CPD:C21738];
NADP+ [CPD:C00006]
Product
(3R)-2'-hydroxyisoflavanone [CPD:C21739];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
This plant enzyme participates in the biosynthesis of the pterocarpan phytoalexins medicarpin, maackiain, and several forms of glyceollin. The enzyme has a strict stereo specificity for the 3R-isoflavanones.
History
EC 1.1.1.348 created 1992 as EC 1.1.1.246, part transferred 2013 to EC 1.1.1.348
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13265  vestitone reductase
Genes
GMX: 100194416 100793687 100794209 547660
GSJ: 114394525
PVU: PHAVU_008G076600g PHAVU_008G076700g
VRA: 106779595
VAR: 108328041
VUN: 114183399
CCAJ: 109802944
MTR: MTR_7g074710
CAM: 101514431
LJA: Lj6g3v0294050.1(Lj6g3v0294050.1)
ADU: 107472037
AIP: 107624299
 » show all
Taxonomy
Reference
1
  Authors
Bless, W. and Barz, W.
  Title
Isolation of pterocarpan synthase, the terminal enzyme of pterocarpan phytoalexin biosynthesis in cell-suspension cultures of Cicer arietinum.
  Journal
FEBS Lett 235:47-50 (1988)
Reference
2  [PMID:8071365]
  Authors
Guo L, Dixon RA, Paiva NL.
  Title
Conversion of vestitone to medicarpin in alfalfa (Medicago sativa L.) is catalyzed by two independent enzymes. Identification, purification, and characterization of vestitone reductase and 7,2'-dihydroxy-4'-methoxyisoflavanol  dehydratase.
  Journal
J Biol Chem 269:22372-8 (1994)
  Sequence
Reference
3  [PMID:7805842]
  Authors
Guo L, Dixon RA, Paiva NL
  Title
The 'pterocarpan synthase' of alfalfa: association and co-induction of vestitone  reductase and 7,2'-dihydroxy-4'-methoxy-isoflavanol (DMI) dehydratase, the two final enzymes in medicarpin biosynthesis.
  Journal
FEBS Lett 356:221-5 (1994)
DOI:10.1016/0014-5793(94)01267-9
  Sequence
Reference
4  [PMID:7625843]
  Authors
Guo L, Paiva NL
  Title
Molecular cloning and expression of alfalfa (Medicago sativa L.) vestitone reductase, the penultimate enzyme in medicarpin biosynthesis.
  Journal
Arch Biochem Biophys 320:353-60 (1995)
DOI:10.1016/0003-9861(95)90019-5
  Sequence
Reference
5  [PMID:17433362]
  Authors
Shao H, Dixon RA, Wang X
  Title
Crystal structure of vestitone reductase from alfalfa (Medicago sativa L.).
  Journal
J Mol Biol 369:265-76 (2007)
DOI:10.1016/j.jmb.2007.03.040
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.348
IUBMB Enzyme Nomenclature: 1.1.1.348
ExPASy - ENZYME nomenclature database: 1.1.1.348
BRENDA, the Enzyme Database: 1.1.1.348
CAS: 118477-70-6

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