KEGG ENZYME: 1.1.1.357

ENZYME: 1.1.1.357

Entry

EC 1.1.1.357                Enzyme

Name

3alpha-hydroxysteroid 3-dehydrogenase;
3alpha-hydroxysteroid dehydrogenase;
AKR1C4 (gene name);
AKR1C2 (gene name);
hsdA (gene name)

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

3alpha-hydroxysteroid:NAD(P)+ 3-oxidoreductase

Reaction(IUBMB)

a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ [RN:R10422 R10423]

Reaction(KEGG)

R10422 > R02476 R04330 R13216;
R10423 > R02477 R08963 R13217

Substrate

3alpha-hydroxysteroid [CPD:C03072];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

3-oxosteroid [CPD:C01876];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The enzyme acts on multiple 3alpha-hydroxysteroids, such as androsterone and 5 alpha-dihydrotestosterone. The mammalian enzymes are involved in inactivation of steroid hormones, while the bacterial enzymes are involved in steroid degradation. This entry stands for enzymes whose stereo-specificity with respect to NAD+ or NADP+ is not known. [cf. EC 1.1.1.50, 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) and EC 1.1.1.213, 3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)].

History

EC 1.1.1.357 created 2013

Pathway

ec00140

Steroid hormone biosynthesis

ec01100

Metabolic pathways

Orthology

K00037

3alpha-hydroxysteroid 3-dehydrogenase / chlordecone reductase

K00089

3alpha-hydroxysteroid 3-dehydrogenase

K00212

20alpha/3alpha-hydroxysteroid dehydrogenase / dihydrodiol dehydrogenase

K04119

aldo-keto reductase family 1 member C3

K19811

3alpha-hydroxysteroid 3-dehydrogenase

Genes

HSA:	1109(AKR1C4) 1645(AKR1C1) 1646(AKR1C2) 8644(AKR1C3)

PTR:	100607996 100612191(AKR1C2) 100612718(AKR1C3) 100612887 450274(AKR1C4) 746545

PPS:	100971300 100986388(AKR1C3) 100986729 100987632 100987989(AKR1C4) 100988315 103783188

GGO:	101144878(AKR1C3) 101146324 101147265(AKR1C8) 101148343 101152195 115936027

PON:	100171658(AKR1C1) 100174617(AKR1C3) 100189776 100432652 100434557(AKR1C4) 100461633 100462259

PPYG:

129006137 129006867 129006869 129006872 129006873 129006874(AKR1C8) 129006876(AKR1C4)

NLE:	100593325 100603603 100604844 100605385 100605934 100606512

HMH:	116457046 116457717 116460197(AKR1C8) 116461800(AKR1C4)

SSYN:

129491830 129491831 129491832 129491834 129491837 129491838

MCC:	100430336 711198(AKR1C1) 711360(AKR1C2) 711440 722483 722485 722490(AKR1C4) 722496

MCF:	101865536(AKR1C1) 102116465 102116844 102117205 102141103 102142023(HSD17B5) 102143072 102144231

MTHB:

126963005 126963006 126963007 126963008 126963009 126963422

MNI:	105490778 105490779 105490780 105490781 105490782 105490783 105494391 112428206

CSAB:

103237807 103237808 103237812 103237813 103237814 103237816

CATY:

105587685 105587688 105587690 105597444 105597446 105597447 105597481

PANU:

100997485 101022585 101022938 101023297 101024707

TGE:	112631307 112631308 112631309 112631314 112631750

MLEU:

105532487 105532488 105532490 105532496 105535734 105535762 105535763

RRO:	104674747 104674748 104678924 104678925

RBB:	108521035 108521036 108532990 108532991 108532992

TFN:	117082052 117082118 117082119 117082359 117083437 117083548 117083560 117087703 117087714

PTEH:

111528130 111528985 113224919

CANG:

105521699 105521700 105521701 105521702 105521704 105521705

CJC:	100404656(AKR1C1) 100405255 100405615(AKR1C4) 100406962 100409025 100409384

SBQ:	101041594 101041915 101042225 101042532 101049957

CIMI:

108285104 108285106 108292323 108292324(AKR1C4) 108292325 108292357

ANAN:

105710839 105710841 105710885 105710886 105710887 105710891

CSYR:

103261879 103261880 103261882 103261883 103263298 103263300

MMUR:

105858219 105858220 105858221 105858224

LCAT:

123623042 123623043 123623044 123623047

PCOQ:

105826891 105826910 105826913

OGA:	100940488 100942491 100967071

MMU:	77337(Akr1c21)

MCAL:

110308148 110308710

MPAH:

110315750 110333790 110333839 110333848

RNO:	291283(Akr1c2) 307092(Akr1c1) 361267(Akr1c15)

MCOC:

116081731 116081947

ANU:	117713832 117713854

MUN:	110539888 110540933 110542801 110560700 132649305

CGE:	100768090 100768376 100770100 100770388

MAUA:

101839651 101841267 101843587

PROB:

127229800 127229804

PLEU:

114707814 114708002 114708030 114708096

MORG:

121432600 121432606 121432658 121432668

MFOT:

126501679 126501680 126501681 126501693 126506800

AAMP:

119816160 119816243 119817583 119826549

NGI:	103725884 103725890 103725892 103725895 103746652

HGL:	101702106 101716553

CPOC:

100722094 100724342 100724620 100727454

CCAN:

109678869 109680334 109680485 109680990

DSP:

122113031

PLOP:

125366785

NCAR:

124961142 124961655

MMMA:

107134068 107150202 107150203

OCU:	100348607(PGER1) 100352716 108176284

OPI:	101524567 101525116 101525279 101525842 101526245 101526664 101526901 101527313 101527569 101527977 118759111

TUP:	102478063 102478495 102491222 102491650

GVR:	103581518 103602898 103610229 103610426

CFA:	497070(AKR1C3)

CLUD:

112658979

VVP:

112933725

VLG:

121496638

NPO:

129514265

AML:

100483758

UMR:

103676234

UAH:

113241124

UAR:	123802294(AKR1C8)

ELK:

111158636

LLV:

125107753

MPUF:

101678776

MNP:

132020017

MLK:

131838054

NVS:	122891768(AKR1C4)

ORO:	101377633 101379669

EJU:

114217720

ZCA:

113921662

MLX:	118021623 118021905

LWW:	102748666 102748964

FCA:	100510801(AKR1C3)

PYU:

121014878

PCOO:

112867557

PBG:

122473163

PVIV:

125169528

LRUF:

124502362

PTG:

102963188

PPAD:

109273789

PUC:

125918575

AJU:

106988139

HHV:

120237198

BTA:	100139386(20ALPHA-HSD) 112441538 112449293 282138(AKR1C4) 506594 527068 538060 617917(AKR1C3) 782884

BOM:	102267155 102269337 102271453 102271733 102272308 102272594 102272882 102273443 102283454 102284205

BIU:	109567627 109568074 109568076 109568077 109568079 109568080 109568087

BBUB:

102389138 102395293 102395635 102396460 102396772 102400645 102404780 102415234 102416534

BBIS:

104985980 104985985 104985990 104985991 104985992 104985994 104985995 104985998 104985999 104986133

CHX:	102176340 102176622 102177638 102186942 102187204 102187504 102187932 102188418 102188684 102189109 102189495 102190498

OAS:	101107119 101107541 101107809 101109111 101109377 101110434 101110693 105614315 106990122 114108656

BTAX:

128058197 128058202 128058203 128058208 128058211 128058212 128058213 128058216

ODA:	120860627 120868601 120869467 120869469 120869471

CCAD:

122447956 122447958 122447959 122448269 122448454 122448455 122448456 122448511 122448512

MBEZ:

129547517 129555459 129559457 129559483 129559564 129559569 129559578 129559602 129559618 129559630 129559646

SSC:	654406(AKR1CL1) 733634(AKR1C1) 733635 733694(AKR1C1)

CFR:	102511678 116656624 116661570

CBAI:

105074137 105074417 105074427 105074728

CDK:	105104488 105104489 105105880

VPC:	102531609 102541232 102541486

BACU:

103002145 103003050

BMUS:

118891073 118891271

LVE:

103069218

OOR:	101273575 101281536

DLE:	111170128 111170129

PCAD:

102978533

PSIU:

116748968 116749098

NASI:

112407550 112407551

ECB:	100057212 100057251 100070491 100070501 100070509 100070570 100070616 111771771 111771772

EPZ:	103540777 103540778 103541472 103541473 103542048

EAI:	106824650 106827241 106827243 106827244 106827468 106846832 106846833 106846834 123281999

MYB:	102254990 102255491

MYD:	102751586 102763642

MMYO:

118672630 118672665

MLF:	102421358 102428982

MDT:	132215226 132215243

PKL:	118702292 118702295

EFUS:

103300835 103300840

MNA:	107542430 107542432

DRO:	112321159 112323007

SHON:

118977133

AJM:

119057712

PDIC:

114492418

PHAS:

123824859 123831227(AKR1C8)

MMF:	118632067 118632135

PPAM:

129084610 129084627

HAI:

109378613

PALE:

102889546

PGIG:

120608988

PVP:

105299187

MJV:	108384939 108393830 108407235 108407237 108407238

TOD:	119244650 119245260

SARA:

101543981 101545704 101551007 101551999 101555320 101555749

SETR:

126013285

LAV:	100659630 100659914 100660861

TMU:	101342771 101360332

ETF:	101653259 101653576

DNM:	101426998 101428157 101429786 101433437 101433871 101435315

GAS:	123247018 123248678

AFZ:

127564339

TVP:	118850247 118850334

OAA:	100085065 100085983

PSS:	102446010 102446253 102446483

CMY:	102946053 119563672

CCAY:

125625831

DCC:	119843778 119849865

CPIC:

101940001 101940260 101940524

TST:

117882842

CABI:

116815619

MRV:	120395343 120395491 120395494 120395542

PVT:	110070400 110071420 110088827

PMUA:

114590236 114604979 114604980 114604981 114605230 114605592

PRAF:

128421905 128421906 128421907 128421908 128421909 128422029

ZVI:	118090693 118090694 118090915

GJA:

107108026

EMC:

129335705

XLA:	100049750(XB5895996.L) 108712557(akr1c4.S) 443571(akr1c3.L)

XTR:	100127714(akr1c1) 100490956 100494522 101733893 116406435 116409681 496490(akr1c4) 734139 779627(akr1c3)

NPR:	108789947 108789949 108799287 108799288 108799289 108799290 108799291 108799292

RTEM:

120921832 120932840 120932842

BBUF:

121003666

BGAR:

122926607

MUO:

115477556

GSH:

117364122

CPLA:

122559456

HOC:

132825023

BBEL:

109473367

PPYR:

116168970

DMK:

116918341

PMEO:

129593135

PVUL:

126823863 130010387

PMAX:

117329343 117329344 117331065

MCAF:

127727738

MMER:

123536211

CTT:	CtCNB1_1322

CTES:

O987_07590

CRJ:	QMY55_06895

SMIO:

CATMQ487_31860

NTC:	KHQ06_28825

NHU:	H0264_27905

NYA:	LTV02_06370

NYU:	D7D52_11570

AACD:

LWP59_16945

ATHM:

L1857_34415

AMOG:

QRX60_45535

ACYN:

ORV05_17285

» show all

Reference

1 [PMID:8172617]

Authors

Deyashiki Y, Ogasawara A, Nakayama T, Nakanishi M, Miyabe Y, Sato K, Hara A

Title

Molecular cloning of two human liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with chlordecone reductase and bile-acid binder.

Journal

Biochem J 299 ( Pt 2):545-52 (1994)
DOI:10.1042/bj2990545

Sequence

[hsa:1645 1109]

Reference

2 [PMID:7650035]

Authors

Khanna M, Qin KN, Wang RW, Cheng KC

Title

Substrate specificity, gene structure, and tissue-specific distribution of multiple human 3 alpha-hydroxysteroid dehydrogenases.

Journal

J Biol Chem 270:20162-8 (1995)
DOI:10.1074/jbc.270.34.20162

Sequence

[hsa:8644]

Reference

3 [PMID:8944761]

Authors

Oppermann UC, Maser E

Title

Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni.

Journal

Eur J Biochem 241:744-9 (1996)
DOI:10.1111/j.1432-1033.1996.00744.x

Sequence

[ctt:CtCNB1_1322]

Reference

4 [PMID:9812981]

Authors

Mobus E, Maser E

Title

Molecular cloning, overexpression, and characterization of steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. A novel member of the short-chain dehydrogenase/reductase superfamily.

Journal

J Biol Chem 273:30888-96 (1998)
DOI:10.1074/jbc.273.47.30888

Sequence

[ctt:CtCNB1_1322]

Reference

5 [PMID:11514561]

Authors

Nahoum V, Gangloff A, Legrand P, Zhu DW, Cantin L, Zhorov BS, Luu-The V, Labrie F, Breton R, Lin SX

Title

Structure of the human 3alpha-hydroxysteroid dehydrogenase type 3 in complex with testosterone and NADP at 1.25-A resolution.

Journal

J Biol Chem 276:42091-8 (2001)
DOI:10.1074/jbc.M105610200